{"id":208,"date":"2025-02-27T10:11:08","date_gmt":"2025-02-27T02:11:08","guid":{"rendered":"https:\/\/www.biowing.com.cn\/?post_type=product&#038;p=208"},"modified":"2025-04-24T05:07:24","modified_gmt":"2025-04-23T21:07:24","slug":"xbjysjhchoxbdnacl","status":"publish","type":"product","link":"https:\/\/www.biowing.com.cn\/index.php\/product\/xbjysjhchoxbdnacl\/","title":{"rendered":"CHO\u7ec6\u80deDNA\u6b8b\u7559\u68c0\u6d4b\u8bd5\u5242\u76d2"},"content":{"rendered":"<pre><strong>CHO \u6b8b\u7559 DNA \u68c0\u6d4b\u8bd5\u5242\u76d2<\/strong><strong>(\u63a2\u9488\u6cd5\uff09<\/strong>\r\n\r\n<strong>\u00a0<\/strong>\r\n\r\n<strong>\u3010\u4ea7\u54c1\u89c4\u683c\u3011<\/strong>\r\n\r\nCHO 100\u00a0100 Reactions\/\u76d2\r\n\r\n<strong>\u3010\u4ea7\u54c1\u8bf4\u660e\u3011<\/strong>\r\n\r\nBiowing\u00ae\u00a0CHO\u00a0\u6b8b\u7559\u00a0DNA\u00a0\u68c0\u6d4b\u8bd5\u5242\u76d2\u7528\u4e8e\u5b9a\u91cf\u68c0\u6d4b\u5404\u79cd\u751f\u7269\u5236\u54c1\u7684\u4e2d\u95f4\u54c1\u3001\u534a\u6210\u54c1\u548c\u6210\u54c1\u4e2d\u00a0CHO\u00a0\u5bbf\u4e3b\u7ec6\u80de\u00a0DNA\u3002\r\n\r\n\u672c\u8bd5\u5242\u76d2\u91c7\u7528\u4e00\u5957\u5f15\u7269\u63a2\u9488\u5728FAM\u901a\u9053\u5b9a\u91cf\u68c0\u6d4b\u6837\u54c1\u4e2d\u00a0CHO\u00a0\u6b8b\u7559\u00a0DNA\uff0c\u53e6\u5916\u4e00\u5957\u5f15\u7269\u63a2\u9488\u5728HEX\u901a\u9053\u68c0\u6d4b\u5185\u53c2DNA\u3002\r\n\r\n\u8be5\u8bd5\u5242\u76d2\u5177\u6709\u4ee5\u4e0b\u7279\u70b9\uff1a\r\n\r\n<strong>\u7075\u654f<\/strong>\uff1a\u6700\u4f4e\u68c0\u6d4b\u9650\u53ef\u4f4e\u81f31\u00a0fg\/\u03bcL\u3002\r\n\r\n<strong>\u7b80\u5355<\/strong>\uff1a\u6807\u66f2\u5b9a\u91cf\u53c2\u8003\u54c1\u9884\u5206\u88c5\u5e76\u51bb\u5e72\uff0c\u7ed8\u5236\u6807\u66f2\u7b80\u5355\u3002\r\n\r\n<strong>\u53ef\u9760<\/strong>\uff1a\u8bd5\u5242\u76d2\u914d\u5957\u6709CHO DNA\u00a0\u5b9a\u91cf\u53c2\u8003\u54c1\uff0c\u5df2\u6eaf\u6e90\u81f3\u56fd\u5bb6\u6807\u51c6\u54c1\uff1b\u62bd\u63d0\u52a0\u5165\u5185\u53c2\u6838\u9178\uff0c\u5168\u8fc7\u7a0b\u8d28\u91cf\u63a7\u5236\u3002\r\n\r\n<strong>\u51c6\u786e\uff1a<\/strong>\u6837\u54c1\u52a0\u6807\u56de\u6536\uff0c\u8bc4\u4f30\u00a0DNA\u00a0\u63d0\u53d6\u7684\u6548\u7387\u3001\u56de\u6536\u7387\u548c\u51c6\u786e\u5ea6\uff0c\u8bc4\u4f30\u9a8c\u8bc1\u5206\u6790\u65b9\u6cd5\u548c\u7cfb\u7edf\u6027\u80fd\uff0c\u8fdb\u4e00\u6b65\u4fdd\u8bc1\u7ed3\u679c\u7684\u51c6\u786e\u6027\u3002\r\n\r\n<strong>\u5feb\u6377<\/strong>\uff1a\u64cd\u4f5c\u65f6\u95f4\u77ed\uff0c3\u00a0h\u5185\u51fa\u7ed3\u679c\u3002\r\n\r\n<strong>\u3010\u8fd0\u8f93\u53ca\u4fdd\u5b58\u6761\u4ef6\u3011<\/strong>\r\n\r\n\u4f4e\u6e29\u51b7\u51bb\u8fd0\u8f93\uff0c-20\u00a0\u2103\u4fdd\u5b58\uff0c\u6709\u6548\u671f1\u5e74\uff1b\u4f7f\u7528\u540e\u4ecd\u5b58\u653e\u5728\r\n\r\n-20\u00a0\u2103\uff0c\u4e0d\u53ef\u53cd\u590d\u51bb\u878d\u8d85\u8fc7\u4e09\u6b21\u3002\r\n\r\n<strong>\u3010\u4ea7\u54c1\u7ec4\u5206\u3011<\/strong>\r\n\r\n<strong>A:<\/strong>\r\n\r\n<strong><img decoding=\"async\" src=\"https:\/\/img03.71360.com\/w3\/m5wzg3\/20240708\/b7b8b8540f2d58496e8e11760ded8290.png\" \/><\/strong>\r\n\r\n<strong>B:<\/strong>\r\n\r\n<strong><img decoding=\"async\" src=\"https:\/\/img03.71360.com\/w3\/m5wzg3\/20240708\/55a21ba151c91da48bc995ef84b9d1df.png\" \/>\u00a0<\/strong>\r\n\r\n<strong>\u6ce8\uff1a<\/strong>\u672c\u8bd5\u5242\u76d2\u63d0\u4f9b\u7684\u5176\u4ed6\u8bd5\u5242\uff0c\u4f7f\u7528\u524d\u5148\u4f4e\u901f\u79bb\u5fc3\u540e\u5c0f\u5fc3\u5f00\u542f\u3002\u4f7f\u7528\u65f6\u8bf7\u4e0a\u4e0b\u8f7b\u67d4\u98a0\u5012\u5341\u6b21\u6df7\u5300\uff0c\u907f\u514d\u8d77\u6ce1\uff0c\u5e76\u4f4e\u901f\u77ed\u6682\u79bb\u5fc3\u540e\u4f7f\u7528\u3002\r\n\r\n<strong>\u3010\u64cd\u4f5c\u6b65\u9aa4\u3011<\/strong>\r\n\r\n1.\u00a0<strong>\u4ea7\u54c1\u7ec4\u5206\u7684\u590d\u6eb6<\/strong>\r\n\r\nBiowing\u00aeCHO Primer&amp;Probe Mix\u3001\u5185\u90e8\u8d28\u63a7\uff08IC\uff09\u3001CHO DNA \u5b9a\u91cf\u53c2\u8003\u54c1\uff081\u00a0pg\/\u03bcL\uff09\u752812000\u00a0rpm \u79bb\u5fc32 min\u540e\uff0c\u5206\u522b\u7528242\u00a0\u03bcL\/\u7ba1\u548c220\u00a0\u03bcL\/\u7ba1\u3001275\u00a0\u03bcL\/\u7ba1RNase Free Water\u590d\u6eb6\uff0c\u6df7\u53001min\u540e\u77ac\u79bb\u3002\r\n\r\n2.\u00a0<strong>qPCR \u53cd\u5e94\u6db2\u7684\u51c6\u5907<\/strong>\r\n\r\n\uff081\uff09\u6839\u636e\u6240\u8981\u68c0\u6d4b\u6837\u54c1\u7684\u6570\u91cf\uff0c\u8ba1\u7b97\u6240\u9700\u53cd\u5e94\u5b54\u6570\uff0c\u6bcf\u4e2a\u6837\u672c\u505a3\u590d\u5b54\u3002\r\n\r\n\u53cd\u5e94\u5b54\u6570=1\u4e2a\u65e0\u6a21\u677f\u5bf9\u7167NTC+1\u4e2a\u9634\u6027\u8d28\u63a7\u00a0NCS+\uff085\u4e2a\u6d53\u5ea6\u68af\u5ea6\u7684\u6807\u51c6\u66f2\u7ebf+\u6837\u672c\u6570+\u52a0\u6807\u56de\u6536\u8d28\u63a7\u00a0ERC\uff09\u00d73\r\n\r\n\uff082\uff09\u6839\u636e\u53cd\u5e94\u5b54\u6570\u8ba1\u7b97\u6240\u9700\u7684qPCR Mix\u00a0\u603b\u91cf\uff08\u9700\u67091\u5b54\u7684\u52a0\u6837\u635f\u5931\u91cf\uff09\uff1a\r\n\r\nMix =\uff08\u53cd\u5e94\u5b54\u6570+1\uff09\u00d720\u00a0\u03bcL\r\n\r\n\uff083\uff09\u5404\u8bd5\u5242\u653e\u5ba4\u6e29\u878d\u5316\uff0c\u5e76\u6839\u636e\u4e0b\u8868\u6240\u793a\u51c6\u5907\u00a0qPCR Mix\uff1a\r\n\r\n\u88682 qPCR Mix\u7684\u914d\u5236\r\n\r\n<img decoding=\"async\" src=\"https:\/\/img03.71360.com\/w3\/m5wzg3\/20240708\/7b4b5febc6d9ce712992ca3d4018bc7b.png\" \/>\r\n\r\n3.\u00a0<strong>\u6837\u672c\u5236\u5907\uff08\u81ea\u5907\uff09<\/strong>\r\n\r\nv\u00a0<strong>\u914d\u5236\u00a0CHO DNA \u6807\u51c6\u66f2\u7ebf<\/strong>\r\n\r\n\uff081\uff09\u53d6\u4e09\u6761\u542bCHO DNA \u6807\u51c6\u66f2\u7ebf\u5b9a\u91cf\u53c2\u8003\u54c1\u76848\u8fde\u7ba1\uff1b\r\n\r\n\uff082\uff095000 rpm\u79bb\u5fc310min\uff1b\r\n\r\n\uff083\uff098\u8fde\u7ba1\u7684\u524d\u4e94\u4e2a\u5b54\u4e2d\u6bcf\u4e2a\u5b54\u52a0\u516510\u03bcL\u00a0RNase Free Water\uff1b\r\n\r\n\uff084\uff09\u6da1\u65cb\u632f\u8361\u6df7\u5300\uff0c\u77ed\u6682\u79bb\u5fc3\u00a05 \u79d2\uff1b\r\n\r\n\uff085\uff09\u6d53\u5ea6\u4f9d\u6b21\u4e3a10\u00a0pg\/\u03bcL\u30011\u00a0pg\/\u03bcL\u3001100 fg\/\u03bcL\u300110 fg\/\u03bcL\u30011\u00a0fg\/\u03bcL\u3002\r\n\r\nv\u00a0<strong>\u5f85\u6d4b\u6837\u54c1\u7684\u5236\u5907<\/strong>\r\n\r\n\uff081\uff09\u53d6\u00a0200 \u03bcL\u00a0\u5f85\u6d4b\u6837\u672c\u52a0\u5165\u00a01.5 mL\u00a0\u5e72\u51c0\u7684\u79bb\u5fc3\u7ba1\u4e2d\uff0c\u518d\u52a0\u5165\u00a010 \u03bcL\u00a0IC\uff0c\u6807\u8bb0\u4e3a\u6837\u54c1\u3002\r\n\r\n\uff082\uff09\u5f85\u6d4b\u6837\u54c1\u8fdb\u884c\u6837\u54c1\u62bd\u63d0\u524d\u5904\u7406\uff0c\u5236\u5907\u6210\u6837\u54c1\u7eaf\u5316\u6db2\u3002\r\n\r\nv\u00a0<strong>\u52a0\u6807\u56de\u6536\u8d28\u63a7\u00a0<\/strong><strong>ERC\u00a0<\/strong><strong>\u7684\u5236\u5907<\/strong>\r\n\r\n\u6839\u636e\u9700\u8981\u8bbe\u7f6e\u00a0ERC\u00a0\u4e2d\u7684\u00a0CHO DNA\u00a0\u52a0\u6837\u6d53\u5ea6\uff08\u4ee5\u5236\u5907\u52a0\u00a010 pg CHO DNA\u00a0\u91cf\u7684\u6837\u54c1\u00a0ERC\u00a0\u4e3a\u4f8b\uff09\uff0c\u5177\u4f53\u64cd\u4f5c\u5982\u4e0b\uff1a\r\n\r\n\uff081\uff09\u53d6\u00a0200 \u03bcL\u00a0\u5f85\u6d4b\u6837\u54c1\u52a0\u5165\u00a01.5 mL\u00a0\u5e72\u51c0\u7684\u79bb\u5fc3\u7ba1\u4e2d\u3002\r\n\r\n\uff082\uff09\u52a0\u5165\u00a010 \u03bcL CHO DNA \u5b9a\u91cf\u53c2\u8003\u54c1\uff081\u00a0pg\/\u03bcL\uff09\uff0c\u518d\u52a0\u5165\u00a010 \u03bcL\u00a0IC\uff0c\u6df7\u5300\uff0c\u6807\u8bb0\u4e3a\u6837\u54c1\u00a0ERC\u3002\u6837\u54c1\u00a0ERC\u00a0\u548c\u540c\u6279\u5f85\u6d4b\u6837\u54c1\u4e00\u8d77\u8fdb\u884c\u6837\u54c1\u62bd\u63d0\u524d\u5904\u7406\uff0c\u5236\u5907\u6210\u6837\u54c1\u00a0ERC\u00a0\u7eaf\u5316\u6db2\u3002\r\n\r\nv\u00a0<strong>\u9634\u6027\u8d28\u63a7\u00a0<\/strong><strong>NCS\u00a0<\/strong><strong>\u7684\u5236\u5907<\/strong>\r\n\r\n\uff081\uff09\u53d6\u00a0200 \u03bcL RNase Free Water\u52a0\u5165\u00a01.5 mL\u00a0\u5e72\u51c0\u7684\u79bb\u5fc3\u7ba1\u4e2d\uff0c\u518d\u52a0\u5165\u00a010 \u03bcL\u00a0IC\uff0c\u6807\u8bb0\u4e3a\u9634\u6027\u8d28\u63a7\u00a0NCS\u3002\r\n\r\n\uff082\uff09\u540c\u5f85\u6d4b\u6837\u54c1\u4e00\u8d77\u8fdb\u884c\u6837\u54c1\u62bd\u63d0\u524d\u5904\u7406\uff0c\u5236\u5907\u6210\u9634\u6027\u8d28\u63a7\u00a0NCS\u00a0\u7eaf\u5316\u6db2\u3002\r\n\r\n<strong>\u6ce8\uff1a<\/strong><strong>\u6837\u672c\u62bd\u63d0\u524d\u5904\u7406\u5177\u4f53\u89c1\u9644\u5f551<\/strong>\r\n\r\n4.\u00a0<strong>\u52a0\u6837<\/strong>\r\n\r\n<strong>qPCR \u53cd\u5e94\u4f53\u7cfb\u53ca\u6761\u4ef6\u53cd\u5e94\u4f53\u7cfb\u4ee530\u00b5L\u4e3a\u4f8b\uff1a<\/strong>\r\n\r\n\uff081\uff09\u9707\u8361\u6df7\u5300\u00a0qPCR Mix\uff0c\u4f4e\u901f\u79bb\u5fc3\uff0c\u5c06\u7ba1\u76d6\u6b8b\u7559\u6db2\u4f53\u6536\u96c6\u81f3\u7ba1\u5e95\u3002\r\n\r\n\uff082\uff09\u5411\u6bcf\u5b54\u53cd\u5e94\u7ba1\/CHO DNA\u00a0\u6807\u51c6\u66f2\u7ebf\u5b9a\u91cf\u53c2\u8003\u54c1\u53cd\u5e94\u7ba1\u4e2d\u5206\u88c5\u00a020\u00a0\u03bcL qPCR Mix\u3002\r\n\r\n\uff083\uff09\u5411\u5df2\u5206\u88c5\u8fc7\u00a0qPCR Mix\u00a0\u7684\u53cd\u5e94\u7ba1\u4e2d\u52a0\u516515\u00a0\u03bcL\u77f3\u8721\u6cb9\u3002\r\n\r\n\uff084\uff09\u5411\u53cd\u5e94\u7ba1\u4e2d\u7a7f\u8fc7\u77f3\u8721\u6cb9\u5c42\u52a0\u6837\uff0c3000 rpm\u00a0\u77ac\u79bb5 s\uff0c\u52a0\u6837\u793a\u4f8b\u5982\u4e0b\uff1a\r\n\r\n\u8868\u00a03\u00a0\u52a0\u6837\u793a\u4f8b\r\n\r\n<img decoding=\"async\" src=\"https:\/\/img03.71360.com\/w3\/m5wzg3\/20240708\/a7cbbe82e59771414b1ffed13257f168.png\" \/>\r\n\r\n\u6ce8\uff1a\u5efa\u8bae\u5176\u4ed6\u4e0d\u540c\u7c7b\u578b\u6837\u672c\uff0c\u5148\u505a\u9002\u5e94\u6027\u9a8c\u8bc1\uff0c\u56e0\u4e3a\u4e0d\u540c\u6837\u672c\u6027\u8d28\u4e0d\u540c\u3002\r\n\r\n<strong>PCR\u00a0<\/strong><strong>\u4eea\u8bbe\u7f6e\u4ee5<\/strong><strong>ABI 7500\u00a0<\/strong><strong>\u4e3a\u4f8b\uff0c\u76f8\u5bf9\u5b9a\u91cf\u6a21\u5f0f\uff0c\u9009\u62e9<\/strong><strong>TaqMan\u00a0<\/strong><strong>\u63a2\u9488\u6cd5\uff1a<\/strong>\r\n\r\n<strong><img decoding=\"async\" src=\"https:\/\/img03.71360.com\/w3\/m5wzg3\/20240708\/0e82f19cee1eea523f0e566e8f238e6f.png\" \/><\/strong>\r\n\r\n<strong>\u3010\u9608\u503c\u8bbe\u7f6e\u3011<\/strong>\r\n\r\n\u4ee5ABI 7500\u00a0\u4e3a\u4f8b\uff0c\u6269\u589e\u66f2\u7ebf\u9009\u62e9\u00a0log\u00a0\u6cd5\uff0c\u57fa\u7ebf\u8bbe\u7f6e3-15\u4e2a\u5faa\u73af\u3002\u5efa\u8bae\u7528\u00a0log\u00a0\u6cd5\u6269\u589e\u66f2\u7ebf\u4fdd\u8bc1\u7ed3\u679c\u7a33\u5b9a\u53ef\u9760\u3002\r\n\r\n\uff081\uff09\u00a0\u5185\u53c2(HEX\/VIC)\u9608\u503c\u8bbe\u5b9a\uff1a\u4ee5\u9634\u6027\u8d28\u63a7\u00a0NCS\u5b9a\u5185\u53c2\u6269\u589e\u66f2\u7ebf\u9608\u503c\uff0c\u5c06\u5185\u53c2\u7684Ct\u00a0\u503c\u5b9a\u4e3a\u00a028\u00b12\u3002\r\n\r\n\uff082\uff09\u00a0CHO(FAM)\u9608\u503c\u8bbe\u5b9a\uff1a\u4ee5\u6807\u66f2\u6700\u9ad8\u6d53\u5ea6\u5b9aCHO\u6269\u589e\u66f2\u7ebf\u9608\u503c\uff0c\u5c06CHO(FAM)\u7684Ct\u503c\u5b9a\u4e3a19\u00b12\u3002\r\n\r\n<strong>\u3010\u7ed3\u679c\u5224\u8bfb\u3011<\/strong>\r\n\r\n\u6839\u636eCHO(FAM) Ct\u00a0\u503c\u5224\u5b9a\u662f\u5426\u53d1\u751fCHO\u6b8b\u7559\uff0c\u5177\u4f53\u6807\u51c6\u5982\u4e0b\uff1a\r\n\r\n<img decoding=\"async\" src=\"https:\/\/img03.71360.com\/w3\/m5wzg3\/20240708\/9d01a5f760c70c3eab9f59be91b274c5.png\" \/>\r\n\r\n1.\u00a0\u68c0\u67e5\u5185\u53c2\u901a\u9053\u6269\u589e\u662f\u5426\u6b63\u5e38\uff0c\u6269\u589e\u66f2\u7ebf\u662f\u5426\u9009\u62e9log\u00a0\u6cd5\u3002\r\n\r\n2.\u00a0\u5185\u53c2\u6269\u589e\u4e0d\u6b63\u5e38\uff0c\u8868\u660e\u00a0PCR\u53cd\u5e94\u672a\u6b63\u5e38\u8fdb\u884c\uff0cPCR\u00a0\u4f53\u7cfb\u53d7\u5230\u5e72\u6270\uff0c\u9700\u91cd\u590d\u68c0\u6d4b\u3002\r\n\r\n3.\u00a0\u5185\u53c2\u6269\u589e\u6b63\u5e38\uff0c\u7ed3\u5408\u00a0Ct\u00a0\u503c\u548c\u6269\u589e\u66f2\u7ebf\u5f62\u6001\u5224\u65ad\u3002\r\n\r\n4.\u00a0\u65e0\u5178\u578b\u6269\u589e\u66f2\u7ebf\uff0c\u53ef\u8ba4\u4e3a\u8be5\u6837\u672c\u4e3aCHO\u65e0\u6b8b\u7559\u3002\u6709\u5178\u578b\u6269\u589e\u66f2\u7ebf\uff0c\u9700\u91cd\u590d\u68c0\u6d4b\uff0c\u91cd\u590d\u7ed3\u679c\u4e0d\u4e00\u81f4\u5224\u4e3a\u65e0\u6b8b\u7559\uff1b\u7ed3\u679c\u4e00\u81f4\uff0c\u5224\u4e3a\u6709\u6b8b\u7559\u3002\r\n\r\n5.\u00a0\u6839\u636e\u5f85\u6d4b\u6837\u54c1\u548c\u6837\u54c1ERC\u7684\u68c0\u6d4b\u7ed3\u679c\u8ba1\u7b97\u52a0\u6837\u56de\u6536\u7387\uff0c\u52a0\u6837\u56de\u6536\u7387\u8981\u6c42\u572850%\uff5e150%\u4e4b\u95f4\uff0c\u5177\u4f53\u7b97\u6cd5\u89c1\u9644\u5f554\u3002\r\n\r\n6.\u00a0\u4e0a\u673a\u7ed3\u675f\u540e\uff0c\u5728\u7a0b\u5e8f\u7684\u53f3\u4e0a\u89d2\u70b9\u51fb\u201cplate setup\u201d\u00a0\u680f\u4e0b\u7684\u201cAssign Targets and Samples\/Assign target(s) to the selected wells\u201d\uff0c\u9009\u4e2d\u6807\u51c6\u54c1\u7684\u53cd\u5e94\u683c\uff0c\u5728\u53f3\u4fa7\u7684\u201cTask\u201d\u680f\u00a0\u4e0b\u9009\u62e9\u201c<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/siteadmin.marketingforce.com\/ueditor\/themes\/default\/images\/spacer.gif\" width=\"14\" height=\"15\" \/>\u00a0\u201d\uff0c\u8bbe\u7f6e\u597d\u6bcf\u4e2a\u68af\u5ea6\u7684\u91cd\u590d\u5b54\uff0c\u5728\u201cQuantity\u201d\u4e0b\u8f93\u5165\u6240\u505a\u7684\u6d53\u5ea6\uff0c\u4f9d\u6b21\u5c06\u6807\u51c6\u54c1\u7684\u68af\u5ea6\u8bbe\u7f6e\u5b8c\u6210\u3002\r\n\r\n7.\u00a0\u70b9\u51fb\u201cAnalysis\u201d\u680f\u4e0b\u7684\u201cStandard Curve\u201d\u5373\u53ef\u8bfb\u53d6\u6807\u51c6\u66f2\u7ebf\u7684R\u00b2\u3001\u6269\u589e\u6548\u7387\uff08Eff%\uff09\u548c\u659c\u7387\uff08Slope\uff09\uff1b\u6b63\u5e38\u7684\u6807\u66f2\uff1aR\u00b2\uff1e0.99\uff1b\u6269\u589e\u6548\u7387\u5728\u00a090%\u2264Eff%\u2264110%\u8303\u56f4\u5185\uff1bSlope\u00a0\u5728-3.4\u00a0\u5de6\u53f3\u3002\r\n\r\n<strong>\u3010\u6ce8\u610f\u4e8b\u9879\u3011<\/strong>\r\n\r\n\u7531\u4e8e\u00a0PCR\u00a0\u53cd\u5e94\u975e\u5e38\u7075\u654f\uff0c\u5b9e\u9a8c\u64cd\u4f5c\u4e2d\u5e94\u6ce8\u610f\u4ee5\u4e0b\u4e8b\u9879\uff0c\u4ee5\u514d\u53d1\u751fDNA\u00a0\u6c61\u67d3\uff1a\r\n\r\n1.\u00a0\u5efa\u8bae\u5206\u533a\u64cd\u4f5c\u548c\u5206\u533a\u5b58\u653e\u3002\r\n\r\nA\u533a\uff1a\u6837\u672cDNA\u63d0\u53d6\uff0c\u5efa\u8bae\u5728\u8d85\u51c0\u5de5\u4f5c\u53f0\u5b8c\u6210\uff1b\r\n\r\nB\u533a\uff1a\u914d\u5236mix\u548cqPCR\u9634\u6027\u52a0\u6837\uff0c\u5efa\u8bae\u5728\u8d85\u51c0\u5de5\u4f5c\u53f0\u5b8c\u6210\uff1b\r\n\r\nC\u533a\uff1a\u6309\u7167\u5148\u52a0\u5f85\u6d4b\u6837\u672c\uff0c\u540e\u914d\u5236\u6807\u66f2\u7684\u987a\u5e8f\u52a0\u6837\uff1b\r\n\r\nD\u533a\uff1aqPCR\u6269\u589e\u68c0\u6d4b\u3002\r\n\r\n2.\u00a0\u5efa\u8bae\u5728\u00a0qPCR\u00a0\u53cd\u5e94\u677f\u4e0a\u9633\u6027\u5bf9\u7167\u7684\u6392\u5e03\u5e94\u8fdc\u79bb\u5f85\u6d4b\u6837\u672c\u548c\u9634\u6027\u5bf9\u7167\uff1b\u4f7f\u7528\u4e0d\u540c\u7684\u79fb\u6db2\u5668\u6dfb\u52a0\u9633\u6027\u5bf9\u7167\u4e0e\u5f85\u6d4b\u6837\u672c\u3001\u9634\u6027\u5bf9\u7167\uff0c\u5e76\u4f7f\u7528\u5e26\u6ee4\u82af\u7684\u67aa\u5934\u8fdb\u884c\u52a0\u6837\u3002\r\n\r\n3.\u00a0\u8bd5\u5242\u76d2\u5f00\u542f\u540e\uff0c\u8bd5\u5242\u00a0Mix\u3001\u9634\u6027\u5bf9\u7167\u5b58\u653e\u5728B\u533a\uff0c\u9633\u6027\u5bf9\u7167\u5e94\u5b58\u653e\u5728C\u533a\u3002\r\n\r\n4. qPCR\u00a0\u5c01\u819c\u65f6\u987b\u53cd\u590d\u538b\u7d27\u3002\r\n\r\n5.\u00a0\u6bcf\u5468\u7528\u6838\u9178\u6e05\u9664\u5242\u6e05\u6d01\u65e0\u83cc\u64cd\u4f5c\u53f0\u3001\u4f20\u9012\u7a97\u548c\u684c\u9762\u3002\r\n\r\n6.\u00a0\u5783\u573e\u5efa\u8bae\u5f53\u573a\u5c01\u95ed\u5e76\u4e22\u5f03\u3002\r\n\r\n7.\u00a0\u5bf9\u5177\u4f53\u6837\u54c1\u7684\u00a0DNA\u00a0\u52a0\u6807\u91cf\u8bbe\u5b9a\u5728\u5176\u65e0\u52a0\u6807\u6d4b\u8bd5\u503c\u7684\u00a02-10\u00a0\u500d\u4e3a\u5b9c\u3002\r\n\r\n8. 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Free Water\r\n\r\n\u00d8\u00a0\u65e0\u6c34\u4e59\u9187\uff08\u5206\u6790\u7eaf\uff09\r\n\r\n\u00d8\u00a0PCR 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