{"id":215,"date":"2025-02-27T10:16:38","date_gmt":"2025-02-27T02:16:38","guid":{"rendered":"https:\/\/www.biowing.com.cn\/?post_type=product&p=215"},"modified":"2025-04-24T05:15:00","modified_gmt":"2025-04-23T21:15:00","slug":"xbjysjhxbxjzjlj","status":"publish","type":"product","link":"https:\/\/www.biowing.com.cn\/index.php\/product\/xbjysjhxbxjzjlj\/","title":{"rendered":"\u7ec6\u80de\u7ec6\u83cc\/\u771f\u83cc\u8054\u68c0\u8bd5\u5242\u76d2"},"content":{"rendered":"
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Reactions\/\u76d2\r\n\r\n\u3010\u4ea7\u54c1\u8bf4\u660e\u3011<\/strong>\r\n\r\n\u57fa\u4e8eTaqMan\u63a2\u9488\u8367\u5149\u5b9a\u91cfPCR\u539f\u7406\u7684\u68c0\u6d4b\u65b9\u6848\uff1a\u9009\u62e9\u7ec6\u83cc\uff08\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u3001\u751f\u5b62\u68ad\u83cc\u3001\u67af\u8349\u7259\u5b62\u6746\u83cc\u3001\u94dc\u7eff\u5047\u80de\u6746\u83cc\uff09\u548c\u771f\u83cc\uff08\u767d\u8272\u5ff5\u73e0\u83cc\u548c\u9ed1\u66f2\u9709\uff09\u57fa\u56e0\u4e2d\u7279\u5f02\u57fa\u56e0\u7247\u6bb5\uff0c\u5206\u522b\u8bbe\u8ba1\u5f15\u7269\u548c\u8367\u5149\u63a2\u9488\uff0c\u5feb\u901f\u68c0\u6d4b4\u79cd\u7ec6\u83cc\u548c2\u79cd\u771f\u83cc\u7684\u57fa\u56e0\u7ec4DNA\u3002\r\n\r\n\u7ed3\u5408\u76f8\u5173\u7684\u6837\u672c\u524d\u5904\u7406\u548cDNA\u62bd\u63d0\u65b9\u6848\uff0c\u672c\u8bd5\u5242\u76d2\u53ef\u4ee5\u68c0\u6d4b\u5404\u7c7b\u751f\u7269\u5236\u54c1\u548c\u7ec6\u80de\u57f9\u517b\u7269\u4e2d\u6f5c\u5728\u7684\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u3001\u751f\u5b62\u68ad\u83cc\u3001\u67af\u8349\u7259\u5b62\u6746\u83cc\u3001\u94dc\u7eff\u5047\u80de\u6746\u83cc\u3001\u767d\u8272\u5ff5\u73e0\u83cc\u548c\u9ed1\u66f2\u9709\u6c61\u67d3\u3002\u00a0 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\u53ef\u9760<\/strong>\uff1a\u62bd\u63d0\u52a0\u5165\u5185\u6807\u6838\u9178\uff0c\u5168\u8fc7\u7a0b\u8d28\u91cf\u63a7\u5236\u3002\r\n\r\n\u00a0\u7a33\u5b9a<\/strong>\uff1a\u5168\u7a0b\u95ed\u7ba1\u64cd\u4f5c\uff0c\u65e0\u4ea4\u53c9\u6c61\u67d3\u3002\r\n\r\n\u5feb\u6377<\/strong>\uff1a\u5355\u7ba1\u53ef\u540c\u65f6\u68c0\u6d4b6\u79cd\u83cc\u79cd\uff0c\u68c0\u6d4b\u6548\u7387\u9ad8\u3002\r\n\r\n\u51c6\u786e<\/strong>\uff1a\u6837\u672c\u8bbe\u7f6e3\u91cd\u590d\uff0c\u907f\u514d\u5047\u9634\u3001\u9633\u6027\u7684\u51fa\u73b0\u3002\r\n\r\n\u3010\u6709\u6548\u671f\u3011<\/strong>\r\n\r\n\u89c4\u5b9a\u50a8\u5b58\u6761\u4ef6\u4e0b6\u4e2a\u6708\u3002\r\n\r\n-20\u00a0\u2103\u53d6\u51fa\u540e\uff0c\u53ef\u907f\u5149\u5b58\u653e\u4e8e4\u00a0\u2103\u7ee7\u7eed\u4f7f\u75281\u5468\u3002\r\n\r\n\u3010\u4ea7\u54c1\u7ec4\u5206\u3011<\/strong>\r\n\r\n\u88681 \u00a0\u00a0\u4ea7\u54c1\u7ec4\u5206\r\n\r\n<\/strong>\r\n\r\n\u6ce8\uff1a<\/strong>\r\n\r\n1\u3001Bacterial&Fungus\u00a0Positive Control\u542b4\u79cd\u7ec6\u83ccDNA\u30012\u79cd\u771f\u83ccDNA\u548c\u5185\u6807DNA\u3002\r\n\r\n2\u3001Internal Standard\u542b\u6709\u5185\u6807DNA\u3002\r\n\r\n\u5728\u5b9e\u9a8c\u524d\u8bf7\u5b8c\u6574\u9605\u8bfb\u672c\u8bf4\u660e\u4e66\uff0c\u52a1\u5fc5\u91cd\u89c6\u6ce8\u610f\u4e8b\u9879\u548c\u65e0\u83cc\u64cd\u4f5c\u89c4\u8303\uff01<\/strong>\r\n\r\n\u00a0<\/strong>\r\n\r\n\u3010\u64cd\u4f5c\u6b65\u9aa4\u3011<\/strong>\r\n\r\n1.<\/strong>\u6837\u672c\u5236\u5907<\/strong>\r\n\r\n\uff081\uff09\u00a0\u6837\u672c\u7684\u6807\u51c6\u5236\u5907\u65b9\u6848\uff1a<\/strong>\u8bf7\u914d\u5408\u4f7f\u7528\u9ad8\u6548\u65e0\u5fae\u751f\u7269\u6c61\u67d3\u7684DNA\u62bd\u63d0\u8bd5\u5242\u76d2\u63d0\u53d6\u6837\u672cDNA\u3002\u6574\u4e2a\u8fc7\u7a0b\u9700\u8981\u9075\u5b88\u65e0\u83cc\u64cd\u4f5c\u89c4\u8303\u3002\u672c\u516c\u53f8\u76ee\u524d\u63a8\u8350\u62bd\u63d0\u8bd5\u5242\u76d2\u89c1\u9644\u5f552\u3002\u76f4\u63a5\u53d620 \u00b5L\u5f85\u6d4b\u6837\u672cDNA\uff0c\u4f5c\u4e3a\u6a21\u677f\u8fdb\u884c\u00a0qPCR\u00a0\u53cd\u5e94\u3002\r\n\r\n\uff082\uff09\u00a0\u524d\u5904\u7406\u9634\u6027\u6837\u672c\u51c6\u5907\u65b9\u6848\uff1a<\/strong>\u5c06RNase Free Water\u5f53\u6210\u6837\u672c\uff0c\u52a0\u5165\u5185\u90e8\u8d28\u63a7\uff08Internal standard\uff09\uff0c\u8fdb\u884c\u6838\u9178\u62bd\u63d0\u3002\r\n\r\n1.\u00a0qPCR\u00a0\u53cd\u5e94\u6db2\u7684\u51c6\u5907<\/strong>\r\n\r\n\r\n\r\n\r\n\uff081\uff09\u00a0\u6839\u636e\u6240\u8981\u68c0\u6d4b\u6837\u54c1\u7684\u6570\u91cf\uff0c\u8ba1\u7b97\u6240\u9700\u53cd\u5e94\u5b54\u6570\uff0c\u6bcf\u4e2a\u6837\u672c\u505a3\u4e2a\u91cd\u590d\u5b54\u3002\r\n\r\n\u53cd\u5e94\u5b54\u6570=1\u4e2a\u9633\u6027PCR\u8d28\u63a7\u00a0PC 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