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\"\u56fe1.jpg\"<\/p>\n

\n

\u5c31\u76ee\u524d\u800c\u8a00\uff0c\u6700\u5e38\u7528\u7684\u7aef\u7c92\u957f\u5ea6\u68c0\u6d4b\u65b9\u6cd5\u5305\u62ec\uff1a\u7aef\u7c92\u672b\u7aef\u9650\u5236\u6027\u7247\u6bb5\u5206\u6790\uff08Terminalrestriction fragment\uff0cTRF\uff09\uff0c\u5b9a\u91cfPCR\uff08qPCR\uff09\uff0c\u8367\u5149\u539f\u4f4d\u6742\u4ea4\uff08Fluorescencein situ hybridization\uff0cFISH\uff09\uff0c\u5355\u94fe\u7aef\u7c92\u957f\u5ea6\u5206\u6790\uff08Singletelomere length analysis\uff0cSTELA\uff09\u548c\u57fa\u4e8e\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\uff08WGS\uff09\u7684\u7aef\u7c92\u957f\u5ea6\u5206\u6790\u3002<\/p>\n

\u57fa\u7840\u7814\u7a76\u901a\u5e38\u68c0\u6d4b\u57f9\u517b\u7684\u7ec6\u80de\u6216\u7ec4\u7ec7\u7aef\u7c92\u957f\u5ea6\uff0c\u4e34\u5e8a\u7814\u7a76\u548c\u6d41\u884c\u75c5\u5b66\u7814\u7a76\u901a\u5e38\u68c0\u6d4b\u5916\u5468\u8840\u6dcb\u5df4\u7ec6\u80de\u7684\u7aef\u7c92\u957f\u5ea6\u3002\u5982\u679c\u6709\u5927\u89c4\u6a21\u7684\u6837\u672c\u9700\u8981\u8fdb\u884c\u9ad8\u901a\u91cf\u68c0\u6d4b\uff0c\u4f8b\u5982\u6d41\u884c\u75c5\u7814\u7a76\u53ca\u4e34\u5e8a\u5927\u6837\u672c\u7684\u7aef\u7c92\u957f\u5ea6\u7b5b\u67e5\uff0cqPCR\u00a0\u662f\u6bd4\u8f83\u597d\u7684\u9009\u62e9\uff0cqPCR\u00a0\u7528\u4e8e\u6d41\u884c\u75c5\u5b66\u7814\u7a76\u6709\u7b80\u4fbf\u3001\u7ecf\u6d4e\u3001\u9ad8\u901a\u91cf\uff0cDNA\u00a0\u7528\u91cf\u5c11\u7b49\u4f18\u52bf\u3002\u4e16\u754c\u4e0a\u6700\u5927\u89c4\u6a21\u7684\uff0810\u4e07\u4eba\uff09\u7aef\u7c92\u957f\u5ea6\u68c0\u6d4b\u9879\u76ee(Lapham et al., 2015)\uff0c\u91c7\u7528\u7684\u5373\u662fqPCR\u65b9\u6cd5\u3002<\/p>\n

\u68c0\u6d4b\u539f\u7406<\/strong><\/p>\n

\u8be5\u9879\u670d\u52a1\u57fa\u4e8e\u7ffc\u548c\u81ea\u4e3b\u7814\u53d1\u7684\u53cc\u8272\u8367\u5149\u5b9a\u91cf\u6cd5\u7aef\u7c92\u957f\u5ea6\u68c0\u6d4b\u8bd5\u5242\u76d2\uff0c\u5e94\u7528\u53cc\u8272\u8367\u5149QPCR\uff0c\u9002\u7528\u4e8e\u8840\u6db2\u3001\u53e3\u8154\u62ed\u5b50\u6216\u7ec6\u80deDNA\u3002\u91c7\u7528\u65b0\u578b\u591a\u62f7\u8d1d\u57fa\u56e0\u4f5c\u4e3a\u5185\u53c2\u57fa\u56e0\uff0c\u5f00\u521b\u4e86\u6269\u589e\u6548\u7387\u9ad8\u3001\u7a33\u5b9a\u6027\u5f3a\u3001\u8bef\u5dee\u66f4\u5c0f\u7684\u53cc\u8272\u8367\u5149qPCR\u6cd5\uff0c\u5e76\u521d\u6b65\u5efa\u7acb\u4e86\u9762\u5411\u4e2d\u56fd\u4eba\u7fa4\u3001\u5305\u542b1500\u4f8b\u9636\u68af\u5f0f\u5e74\u9f84\u7ec4\u8840\u6837DNA\u7aef\u7c92\u957f\u5ea6\u6570\u636e\u5e93\u3002<\/p>\n

\"\u56fe2.jpg\"\"\u56fe3.jpg\"<\/p>\n

\u670d\u52a1\u6d41\u7a0b<\/strong><\/p>\n

1.\u6837\u672cDNA\u63d0\u53d6\uff1b<\/p>\n

2.DNA\u8d28\u63a7\uff1b<\/p>\n

3.qPCR\u6269\u589e\u3002<\/p>\n

\u63d0\u4f9b\u7ed3\u679c<\/strong><\/p>\n

\u6839\u636e\u5b9a\u91cfPCR\u7ed3\u679c\uff0c\u8ba1\u7b97\u51fa\u7684T\/S\u6bd4\u503c\u3002<\/p>\n

\u6837\u672c\u8981\u6c42<\/strong><\/p>\n

1\u3001\u82e5\u6837\u54c1\u4e3a\u8840\u6db2\u57fa\u56e0\u7ec4DNA\uff0c\u8981\u6c42\uff1aDNA\u6d53\u5ea6\u226520ng\/ul\uff0c\u4f53\u79ef>20ul\u3002\u7eaf\u5ea6OD260\/OD280\u00a0\u57281.8\uff5e2.0\u4e4b\u95f4\u3002<\/p>\n

2\u3001\u82e5\u5206\u578b\u6837\u54c1\u4e3a\u8840\u6837\uff0c\u6837\u672c\u8981\u6c42\uff1a\u8840\u6db2\u6837\u54c1\uff0c\u4f53\u79ef\u5927\u4e8e500ul\uff0c\u53ea\u80fd\u91c7\u7528EDTA\u6297\u51dd\u5242\u6297\u51dd\uff0c\u9001\u6837\u8fc7\u7a0b\u4e2d\u8bf7\u6ce8\u610f\u4fdd\u6301\u4f4e\u6e29\uff0c\u9632\u6b62DNA\u964d\u89e3\u3002\u63d0\u53d6DNA\u5c06\u6536\u53d6DNA\u63d0\u53d6\u8d39\u3002<\/p>\n

3\u3001\u82e5\u4e3a\u553e\u6db2\u6837\u672c\uff0c\u9700\u4fdd\u5b58\u4e8e\u4e13\u7528\u7ba1\u4e2d\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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