{"id":418,"date":"2025-03-27T15:42:07","date_gmt":"2025-03-27T07:42:07","guid":{"rendered":"https:\/\/www.biowing.com.cn\/?post_type=product&p=418"},"modified":"2025-06-09T11:55:49","modified_gmt":"2025-06-09T03:55:49","slug":"sydwsjhdnarnagtq","status":"publish","type":"product","link":"https:\/\/www.biowing.com.cn\/index.php\/product\/sydwsjhdnarnagtq\/","title":{"rendered":"DNA\/RNA\u5171\u63d0\u53d6\u8bd5\u5242\u76d2"},"content":{"rendered":"

1\/<\/strong>\u4ea7\u54c1\u6982\u8ff0<\/strong><\/p>\n

\u672c\u8bd5\u5242\u76d2\u9002\u7528\u4e8e\u4ece\u8840\u6db2\u3001\u7ec4\u7ec7\u3001\u7caa\u4fbf\u7b49\u591a\u79cd\u6837\u672c\u4e2d\u5feb\u901f\u63d0\u53d6\u9ad8\u7eaf\u5ea6\u7684\u75c5\u6bd2\u3001\u7ec6\u83cc\u3001\u771f\u83cc DNA\/ RNA\u3002\u8bd5\u5242\u76d2\u91c7\u7528\u72ec\u7279\u6837\u672c\u5904\u7406\u65b9\u6848 \uff0c\u642d\u914d\u6838\u9178\u4fdd\u62a4\u5242\u53ef\u663e\u8457\u63d0\u9ad8\u5fae\u91cf\u6838\u9178\u7684\u56de\u6536\u7387\u53ca\u4e25\u683c\u8d28\u63a7\u62bd\u63d0\u8fc7\u7a0b\u3002\u7ecf\u8fc7\u7845\u80f6\u819c\u7684\u7279\u5f02\u6027\u5438\u9644 \uff0c\u53ef\u5feb\u901f\u9ad8\u6548\u7eaf\u5316\u75c5\u6bd2\u3001\u7ec6\u83cc \u3001\u771f\u83cc DNA\/RNA\u3002 \u6837\u672c\u517c\u5bb9\u6027\u5e7f \uff0c\u83b7\u5f97\u7684\u6838\u9178\u5f97\u7387\u9ad8 \uff0c\u7eaf\u5ea6\u9ad8 \uff0c\u53ef\u76f4\u63a5\u7528\u4e8e\u9006\u8f6c\u5f55\u3001PCR\u3001\u8367\u5149\u5b9a\u91cfPCR\u7b49\u4e0b\u6e38\u76f8\u5173\u5b9e\u9a8c\u3002<\/p>\n

02\/<\/strong>\u4ea7\u54c1\u7ec4\u5206<\/p>\n

 <\/p>\n\n\n\n\n\n\n
 <\/td>\n\u7ec4\u5206<\/strong><\/td>\nPRE.M\/R -001<\/strong><\/p>\n

(50<\/strong> rxns<\/strong>)<\/td>\n<\/tr>\n

BOX 1<\/td>\n\u901a\u7528\u88c2\u89e3\u6db2<\/p>\n

\u7caa\u4fbf\u4e13\u7528\u88c2\u89e3\u6db2<\/td>\n

35 ml<\/p>\n

35 ml<\/td>\n<\/tr>\n

BOX 2<\/td>\n\u6838\u9178\u4fdd\u62a4\u5242<\/p>\n

\u6eb6\u89e3\u6db2<\/p>\n

Proteinase\u00a0K<\/td>\n

1.5 ml<\/p>\n

1.2 ml<\/td>\n<\/tr>\n

 <\/p>\n

 <\/p>\n

BOX 3<\/td>\n

Buffer \u88c2\u89e3\u6db2<\/p>\n

Buffer \u6e05\u6d17\u6db21<\/p>\n

Buffer \u6e05\u6d17\u6db22<\/p>\n

RNase-free ddH2O<\/p>\n

DNA\/RNA Columns (each in a 2 ml Collection Tube) RNase-free Collection Tubes 1.5 ml<\/td>\n

18 ml<\/p>\n

27 ml<\/p>\n

9 ml<\/p>\n

6 ml\u00a0 50\u4e2a 50\u4e2a<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u901a\u7528\u88c2\u89e3\u6db2\uff1a\u88c2\u89e3\u8840\u6db2\u3001\u7ec4\u7ec7\u7b49\u6837\u672c \uff1b \u7caa\u4fbf\u4e13\u7528\u88c2\u89e3\u6db2 \uff1a\u88c2\u89e3\u7caa\u4fbf\u6837\u672c \uff1b<\/p>\n

\u6838\u9178\u4fdd\u62a4\u5242 \uff1a\u63d0\u9ad8\u5fae\u91cf\u6838\u9178\u7684\u56de\u6536\u7387\u53ca\u4e25\u683c\u8d28\u63a7\u62bd\u63d0\u8fc7\u7a0b \uff1b \u6eb6\u89e3\u6db2 \uff1a\u590d\u6eb6\u6838\u9178\u4fdd\u62a4\u5242 \uff1b<\/p>\n

Proteinase K \uff1a\u6d88\u5316\u86cb\u767d\u8d28 \uff1b<\/p>\n

Buffer \u88c2\u89e3\u6db2 \uff1a\u88c2\u89e3\u6837\u672c\u5e76\u63d0\u4f9b\u6838\u9178\u7ed3\u5408\u6761\u4ef6 \uff1b Buffer\u6e05\u6d17\u6db21 \uff1a\u53bb\u9664\u6b8b\u7559\u86cb\u767d\u548c\u5176\u4ed6\u6742\u8d28 \uff1b<\/p>\n

Buffer\u6e05\u6d17\u6db22 \uff1a\u53bb\u9664\u6b8b\u7559\u76d0\u79bb\u5b50 \uff1b<\/p>\n

RNase-free ddH2O \uff1a\u6d17\u8131\u5438\u9644\u819c\u4e0a\u7684DNA\/RNA \uff1b DNA\/RNA Columns \uff1a\u7279\u5f02\u6027\u5438\u9644DNA\/RNA \uff1b<\/p>\n

Collection Tubes 2 ml \uff1a\u6536\u96c6\u6ee4\u6db2 \uff1b<\/p>\n

RNase-free Collection Tubes 1.5 ml \uff1a\u6536\u96c6DNA\/RNA\u3002<\/p>\n

 <\/p>\n

03\/<\/strong>\u4fdd\u5b58\u6761\u4ef6<\/p>\n

BOX 1 \uff1a15 ~ 25\u2103\u4fdd\u5b58 \uff0c\u5ba4\u6e29\u8fd0\u8f93 \uff1b<\/p>\n

BOX 2 \uff1a-30 ~ -15\u2103\u4fdd\u5b58 \uff0c \u22640\u2103\u8fd0\u8f93 \uff1b<\/p>\n

BOX 3 \uff1a15 ~ 25\u2103\u4fdd\u5b58 \uff0c\u5ba4\u6e29\u8fd0\u8f93\u3002<\/p>\n

 <\/p>\n

04\/<\/strong>\u9002\u7528\u8303\u56f4<\/p>\n

\u53ef\u7528\u4e8e\u4ee5\u4e0b\u6837\u672c\u75c5\u6bd2\u3001\u7ec6\u83cc\u3001\u771f\u83ccDNA\/RNA\u63d0\u53d6<\/p>\n

 <\/p>\n

 <\/p>\n\n\n\n\n\n\n\n
\u6837\u672c\u7c7b\u522b<\/strong><\/td>\n\u6837\u672c\u540d\u79f0<\/strong><\/td>\n<\/tr>\n
\u8840\u6db2<\/td>\n\u5168\u8840\u3001\u8840\u6e05\u3001\u8840\u6d46<\/td>\n<\/tr>\n
\u7ec4\u7ec7<\/td>\n\u809d\u810f\u3001\u80ba\u3001\u6c14\u7ba1\u7b49<\/td>\n<\/tr>\n
\u7caa\u4fbf<\/td>\n\u7caa\u4fbf\u3001\u76f2\u80a0\u5185\u5bb9\u7269<\/td>\n<\/tr>\n
\u5176\u4ed6<\/td>\n\u7c89\u5c18\u53ca\u57ab\u6599\u3001 \u53e3\u8154\u62ed\u5b50\u7b49<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

05\/<\/strong>\u81ea\u5907\u6750\u6599<\/p>\n

\u65e0\u6c34\u4e59\u9187\u3001\u03b2-\u5def\u57fa\u4e59\u9187\u3001Lysozyme\uff0cRNase-free \u5438\u5934\u30011.5 ml RNase-free\u79bb\u5fc3\u7ba1\u3001\u79bb\u5fc3\u673a\u3001 \u7814\u78e8\u7834\u788e\u4eea\u3001\u6da1\u65cb\u632f\u8361\u4eea\u3001\u79fb\u6db2\u5668\u7b49\u3002<\/p>\n

 <\/p>\n

06\/<\/strong>\u6ce8\u610f\u4e8b\u9879<\/p>\n

    \n
  1. \u9996\u6b21\u4f7f\u7528\u524d \uff0c\u8bf7\u53c2\u7167\u74f6\u4e0a\u6807\u7b7e\u5728\u901a\u7528\u88c2\u89e3\u6db2\u4e2d\u52a0\u5165\u6307\u5b9a\u91cf\u7684\u03b2-\u5def\u57fa\u4e59\u9187 \uff0c\u6838\u9178\u4fdd\u62a4\u5242\u4e2d\u52a0\u5165\u6307 \u5b9a\u91cf\u7684\u6eb6\u89e3\u6db2 \uff0cBuffer \u6e05\u6d17\u6db2 1 \u4e0e Buffer \u6e05\u6d17\u6db2 2 \u4e2d\u52a0\u5165\u6307\u5b9a\u91cf\u7684\u65e0\u6c34\u4e59\u9187 \uff0c\u5e76\u8fdb\u884c\u6807\u6ce8\u3002<\/li>\n<\/ol>\n\n\n\n\n\n\n\n
    \u7ec4\u5206<\/strong><\/td>\n\u52a0\u5165\u91cf<\/strong><\/td>\n<\/tr>\n
    \u901a\u7528\u88c2\u89e3\u6db2<\/td>\n1 %\u7ec8\u6d53\u5ea6<\/td>\n<\/tr>\n
    \u6838\u9178\u4fdd\u62a4\u5242<\/td>\n1.5ml<\/td>\n<\/tr>\n
    Buffer \u6e05\u6d17\u6db21<\/td>\n18 ml<\/td>\n<\/tr>\n
    Buffer \u6e05\u6d17\u6db22<\/td>\n36 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u25b2\u901a\u7528\u88c2\u89e3\u6db2\u52a0\u5165\u03b2-\u5def\u57fa\u4e59\u9187\u540e\u53ef\u57282 ~ 8\u2103\u4fdd\u5b581\u4e2a\u6708 \uff0c\u5efa\u8bae\u6309\u7167\u7528\u91cf\u73b0\u914d\u73b0\u7528\u3002\u901a\u7528\u88c2\u89e3\u6db2\u5728\u50a8\u5b58 \u65f6\u53ef\u80fd\u4f1a\u5f62\u6210\u6c89\u6dc0 \uff0c\u5982\u679c\u6709\u6c89\u6dc0\u51fa\u73b0 \uff0c\u8bf7\u4e8e60\u2103\u52a0\u70ed\u6eb6\u89e3 \uff0c\u7136\u540e\u5f85\u6062\u590d\u81f3\u5ba4\u6e29\u540e\u4f7f\u7528\u3002<\/p>\n

      \n
    1. \u8bd5\u5242\u76d2\u4e2d\u542b\u6709\u4f4e\u6e29\u7ec4\u5206 Proteinase K \uff0c\u6536\u5230\u540e\u5e94\u7f6e\u4e8e-30 ~ -15\u2103\u4fdd\u5b58\u3002<\/li>\n
    2. \u8bd5\u5242\u76d2\u4e2d\u542b\u6709\u4f4e\u6e29\u7ec4\u5206\u6838\u9178\u4fdd\u62a4\u5242 \uff0c\u4f7f\u7528\u524d\u8bf7\u7528\u8bd5\u5242\u76d2\u4e2d\u6eb6\u89e3\u6db2\u8fdb\u884c\u590d\u6eb6 \uff0c\u4f7f\u7528\u524d\u5145\u5206 \u6df7\u5300 \uff0c\u6309\u7167\u5b9e\u9645\u4f7f\u7528\u60c5\u51b5\u5206\u88c5\u5230RNase-free\u7684\u79bb\u5fc3\u7ba1\u4e2d \uff0c\u5e76\u7f6e\u4e8e-30 ~ -15\u2103\u4fdd\u5b58 \uff0c\u907f\u514d\u53cd\u590d \u51bb\u878d\u3002<\/li>\n
    3. \u6837\u672c\u4f7f\u7528\u524d\u9700\u5e73\u8861\u81f3\u5ba4\u6e29\u3002<\/li>\n
    4. \u6240\u6709\u64cd\u4f5c\u6b65\u9aa4 \uff0c\u5747\u5728\u5ba4\u6e29(15 ~ 25\u2103)\u4e0b\u8fdb\u884c\u3002<\/li>\n
    5. \u75c5\u6bd2\u5177\u6709\u8f83\u5f3a\u7684\u611f\u67d3\u80fd\u529b \uff0c\u64cd\u4f5c\u524d\u5fc5\u987b\u505a\u597d\u5404\u79cd\u9632\u5fa1\u63aa\u65bd\u3002<\/li>\n
    6. \u6837\u672c\u7981\u6b62\u53cd\u590d\u51bb\u878d \uff0c\u907f\u514d\u63d0\u53d6\u7684\u75c5\u6bd2DNA\/RNA\u964d\u89e3\u6216\u4ea7\u91cf\u4e0b\u964d\u3002<\/li>\n
    7. \u4f7f\u7528\u672c\u8bd5\u5242\u76d2\u65f6 \uff0c\u8bf7\u7a7f\u6234\u5b9e\u9a8c\u670d\u3001\u4e00\u6b21\u6027\u4e73\u80f6\u624b\u5957\u3001\u4e00\u6b21\u6027\u53e3\u7f69 \uff0c\u4f7f\u7528RNase-free\u8017\u6750\u7b49 \uff0c \u6700\u5927\u7a0b\u5ea6\u907f\u514dRNase\u6c61\u67d3\u3002<\/li>\n<\/ol>\n

       <\/p>\n

      07\/<\/strong>\u5b9e\u9a8c\u539f\u7406\u4e0e\u6d41\u7a0b\u6982\u8981<\/p>\n

       <\/p>\n

      \u88c2\u89e3\u6837\u672c \uff1a<\/strong>\u53c2\u800308-1\u8fdb\u884c\u6837\u672c\u524d\u5904\u7406 \uff0c\u540e\u7eed\u4f9d\u6b21\u52a0\u5165 300 \u03bcl Buffer \u88c2\u89e3\u6db2\u3001\u6838\u9178\u4fdd\u62a4\u5242\u3001300 \u03bcl \u524d\u5904\u7406\u6db2\u6da1\u65cb\u6df7\u5300 \uff0c\u5ba4\u6e29\u9759\u7f6e5 min<\/p>\n

      \u8c03\u8282\u7ed3\u5408\u6761\u4ef6 \uff1a\u52a0\u5165200 \u03bcl\u65e0\u6c34\u4e59\u9187 \uff0c\u6da1\u65cb\u6df7\u530015 – 30 sec \uff0c\u77ed\u6682\u79bb\u5fc3\u6536\u96c6\u7ba1\u76d6 \u53ca\u7ba1\u58c1\u4e0a\u7684\u6db2\u4f53<\/p>\n

      \u5438\u9644\u6838\u9178<\/strong> \uff1a<\/strong>\u8f6c\u79fb\u6df7\u5408\u6db2\u81f3DNA\/RNA Columns \uff0c12,000 rpm (13,800 \u00d7 g)\u79bb\u5fc3<\/p>\n

      1 min<\/p>\n

      \u53bb\u9664\u6742\u8d28<\/strong> \uff1a<\/strong> \u52a0\u5165700 \u03bcl Buffer\u6e05\u6d17\u6db21(\u5df2\u52a0\u5165\u65e0\u6c34\u4e59\u9187) \uff0c12,000 rpm (13,800 \u00d7 g)\u79bb\u5fc330 sec\uff1b\u52a0\u5165700 \u03bcl Buffer\u6e05\u6d17\u6db22(\u5df2\u52a0\u5165\u65e0\u6c34\u4e59\u9187) \uff0c12,000 rpm (13,800 \u00d7 g )\u79bb\u5fc330sec\uff0c 12,000rpm\uff0813\uff0c800\u00d7 g\uff09\u7a7a\u67f1\u79bb\u5fc32min<\/p>\n

       <\/p>\n

      \u6d17\u8131<\/strong>DNA\/RNA<\/strong>\uff1a<\/strong>\u52a0\u516530 – 50 \u03bcl RNase-free ddH2O\u00a0 \uff0c12,000 rpm (13,800 \u00d7 g) \u79bb\u5fc31 min<\/p>\n

       <\/p>\n

       <\/p>\n

      08\/<\/strong>\u5b9e\u9a8c\u6d41\u7a0b\u00a0 08-1\/<\/strong>\u6837\u672c\u524d\u5904\u7406<\/p>\n

      \u25c6\u8840\u6db2\uff1a\u5c06\u7814\u78e8\u7ba1\u52a0\u597d\u7814\u78e8\u73e0\u540e\uff0c\u53d6 100 \u03bcl\u8840\u6db2(\u5982\u6837\u672c\u91cf\u4e0d\u8db3\uff0c\u4f7f\u7528PBS\u6216\u751f\u7406\u76d0\u6c34\u8865\u8db3\u81f3\u00a0 100 \u03bcl)\u53ca700 \u03bcl \u901a\u7528\u88c2\u89e3\u6db2\u52a0\u5165\u5176\u4e2d\uff0c\u9707\u8361\u6df7\u5300\u540e\u4e0a\u673a\u7814\u78e8\uff1b\u5c06\u7814\u78e8\u597d\u7684\u6837\u672c12,000 rpm 5 min\u79bb\u53bb\u6d6e\u672b\uff0c\u63a5\u7740\u52a0\u5165 300 \u03bcl 20 \u03bcg\/\u03bcl\u7684Lysozyme\u6eb6\u6db2 \uff0c\u9707\u8361\u6df7\u5300\u540e\u7f6e\u4e8e\u91d1\u5c5e\u6d7437 \u2103\u00a0\u00a0\u00a0 \u9707\u8361\u5b75\u80b2 1h-1h 30min\uff1b\u63a5\u7740\u52a0\u516520 \u03bcl Proteinase K\uff0c56\u2103\u5b75\u80b210 min\u3002<\/p>\n

      \u25c6\u7ec4\u7ec7\uff1a\u5c06\u7814\u78e8\u7ba1\u52a0\u597d\u7814\u78e8\u73e0\u540e\uff0c\u79f0\u53d620-50 mg\u7ec4\u7ec7\u6837\u672c\u52a0\u5165\uff0c\u7ee7\u800c\u52a0\u5165700 \u03bcl \u901a\u7528\u88c2\u89e3\u6db2\uff0c \u9707\u8361\u6df7\u5300\u540e\u4e0a\u673a\u7814\u78e8\uff1b\u5c06\u7814\u78e8\u597d\u7684\u6837\u672c12,000 rpm 5 min\u79bb\u53bb\u6d6e\u672b\uff0c\u63a5\u7740\u52a0\u5165 300 \u03bcl 20<\/p>\n

      \u03bcg\/\u03bcl\u7684Lysozyme\u6eb6\u6db2\uff0c\u9707\u8361\u6df7\u5300\u540e\u7f6e\u4e8e\u91d1\u5c5e\u6d7437 \u2103\u9707\u8361\u5b75\u80b2 1h-1h 30min\uff1b\u63a5\u7740\u52a0\u516520 \u03bcl Proteinase K\uff0c56\u2103\u5b75\u80b210 min\u3002<\/p>\n

      \u25c6\u7caa\u4fbf\uff1a\u53d62-3\u7c92\u7caa\u4fbf\u621620-30 mg\u76f2\u80a0\u5185\u5bb9\u7269\u52a0\u5165\u52301.5 ml\u79bb\u5fc3\u7ba1\u4e2d\uff0c\u52a0\u5165700 \u03bcl \u7caa\u4fbf\u4e13\u7528\u00a0\u00a0\u00a0\u00a0 \u88c2\u89e3\u6db2\uff0c\u9707\u8361\u6df7\u530015 s\u540e\u9759\u7f6e10 min\uff0c\u77ac\u65f6\u79bb\u5fc3\uff0c\u53d6\u4e0a\u6e05\u6db2\u52a0\u5165\u5230\u5df2\u52a0\u5165\u7814\u78e8\u73e0\u7684\u7814\u78e8\u7ba1\u4e2d\uff0c \u4e0a\u673a\u7814\u78e8\uff1b\u5c06\u7814\u78e8\u597d\u7684\u6837\u672c12,000 rpm 5 min\u79bb\u53bb\u6d6e\u672b\uff0c\u63a5\u7740\u52a0\u5165 300 \u03bcl 20 \u03bcg\/\u03bcl\u7684<\/p>\n

      Lysozyme\u6eb6\u6db2\uff0c\u9707\u8361\u6df7\u5300\u540e\u7f6e\u4e8e\u91d1\u5c5e\u6d7437 \u2103\u9707\u8361\u5b75\u80b2 1h-1h 30min\uff1b\u63a5\u7740\u52a0\u516520 \u03bcl Proteinase K\uff0c56\u2103\u5b75\u80b210 min\u3002<\/p>\n

      \u25c6\u7c89\u5c18\u53ca\u57ab\u6599\u7b49\u5176\u4ed6\u6837\u672c\uff1a\u75282-3\u4e2a\u6d01\u51c0\u68c9\u7b7e\u5728\u52a8\u7269\u623f\u901a\u98ce\u6a71\u53e3\u6216\u96c6\u5c18\u677f\u4e0a\u522e\u53bb\u7070\u5c18\u6216\u53d63-5\u9897\u7389\u7c73\u82af\u57ab\u6599\uff0c\u5c06\u53d6\u597d\u7070\u5c18\u7684\u68c9\u7b7e\u5934\u6216\u7389\u7c73\u82af\u57ab\u6599\u7f6e\u4e8e700 \u03bcl \u901a\u7528\u88c2\u89e3\u6db2\u4e2d\uff0c\u9707\u8361\u6df7\u5300\u540e\u77ac\u65f6\u79bb\u5fc3\uff1b\u5438\u53d6\u7c89\u5c18\u4e0a\u6e05\u6db2\u6216\u7389\u7c73\u82af\u57ab\u6599\u4e0a\u6e05\uff0c\u52a0\u5165\u5230\u52a0\u597d\u7814\u78e8\u73e0\u7684\u7814\u78e8\u7ba1\u4e2d\uff0c\u9707\u8361\u6df7\u5300\u540e\u4e0a\u673a\u7814\u78e8\uff1b\u5c06\u7814\u78e8\u597d\u7684\u6837\u672c12,000 rpm 5 min\u79bb\u53bb\u6d6e\u672b\uff0c\u63a5\u7740\u52a0\u5165300 \u03bcl 20 \u03bcg\/\u03bcl \u7684Lysozyme\u6eb6\u6db2\uff0c\u9707\u8361\u6df7\u5300\u540e\u7f6e\u4e8e37\u2103\u9707\u8361\u5b75\u80b2 1h-1h 30min\uff1b\u63a5\u7740\u52a0\u516520 \u03bcl Proteinase K\uff0c56\u2103\u5b75\u80b210 min\u3002<\/p>\n

       <\/p>\n

       <\/p>\n

      08-2\/DNA\/RNA<\/strong>\u63d0\u53d6<\/p>\n

      \u4ee5\u4e0b\u8fc7\u7a0b\u5747\u5728\u751f\u7269\u5b89\u5168\u67dc\u4e2d\u8fdb\u884c<\/p>\n

        \n
      1. \u5411RNase-free\u7ba1\u4e2d\u4f9d\u6b21\u52a0\u5165300 \u03bc l Buffer \u88c2\u89e3\u6db2\u300125 \u03bcl \u6838\u9178\u4fdd\u62a4\u5242\u3001300 \u03bcl Buffer \u524d\u5904\u7406\u6db2 \uff0c \u6da1\u65cb\u6df7\u530015 – 30 sec \uff0c\u77ed\u6682\u79bb\u5fc3\u6536\u96c6\u7ba1\u76d6\u53ca\u7ba1\u58c1\u4e0a\u7684\u6db2\u4f53 \uff0c\u5ba4\u6e29\u9759\u7f6e5 min\u3002<\/li>\n
      2. \u52a0\u5165200 \u03bcl \u65e0\u6c34\u4e59\u9187 \uff0c\u6da1\u65cb\u6df7\u530015 – 30 sec \uff0c\u77ed\u6682\u79bb\u5fc3\u6536\u96c6\u7ba1\u76d6\u53ca\u7ba1\u58c1\u4e0a\u7684\u6db2\u4f53\u3002<\/li>\n<\/ol>\n

        \u25b2\u82e5\u6b64\u65f6\u6709\u6742\u8d28\u6c89\u6dc0 \uff0c\u5c5e\u6b63\u5e38\u73b0\u8c61 \uff0c\u53ef\u76f4\u63a5\u8fdb\u884c\u540e\u7eed\u5b9e\u9a8c\u3002<\/p>\n

          \n
        1. \u5c06\u4e0a\u8ff0\u6df7\u5408\u6db2\u5168\u90e8\u8f6c\u79fb\u81f3DNA\/RNA Columns (DNA\/RNA Columns \u5df2\u653e\u5165\u6536\u96c6\u7ba1) \uff0c 12,000 rpm (13,800 \u00d7 g)\u79bb\u5fc31 min \uff0c\u5f03\u6ee4\u6db2\u3002<\/li>\n
        2. \u5411DNA\/RNA Columns\u4e2d\u52a0\u5165700 \u03bcl Buffer \u6e05\u6d17\u6db21(\u8bf7\u5148\u68c0\u67e5\u662f\u5426\u5df2\u52a0\u5165\u65e0\u6c34\u4e59\u9187) \uff0c 12,000 rpm (13,800 \u00d7 g)\u79bb\u5fc330 sec \uff0c\u5f03\u6ee4\u6db2\u3002<\/li>\n
        3. \u5411DNA\/RNA Columns\u4e2d\u52a0\u5165700 \u03bcl Buffer \u6e05\u6d17\u6db22 (\u8bf7\u5148\u68c0\u67e5\u662f\u5426\u5df2\u52a0\u5165\u65e0\u6c34\u4e59 \u9187) \uff0c 12,000 rpm ( 13,800 \u00d7 g)\u79bb\u5fc330 sec \uff0c\u5f03\u6ee4\u6db2\u3002<\/li>\n
        4. 12,000rpm\u00a0(\u00a013,800\u00a0\u00d7\u00a0g)\u7a7a\u67f1\u79bb\u5fc32\u00a0min\u3002<\/li>\n
        5. \u5c0f\u5fc3\u5c06DNA\/RNA Columns\u8f6c\u79fb\u81f3\u65b0\u7684RNase-free Collection Tubes 1.5 ml (\u8bd5\u5242\u76d2\u63d0\u4f9b) \u4e2d\uff0c\u5411\u819c\u4e2d\u592e\u60ac\u7a7a\u52a0\u516530 – 50 \u03bcl\u7684RNase-free ddH2O \uff0c\u5ba4\u6e29\u9759\u7f6e1 min \uff0c12,000 rpm(13,800\u00d7\u00a0g)\u79bb\u5fc31\u00a0min\u3002<\/li>\n
        6. \u5f03\u53bbDNA\/RNA Columns \uff0c\u63d0\u53d6\u7684DNA\/RNA\u53ef\u76f4\u63a5\u7528\u4e8e\u540e\u7eed\u68c0\u6d4b \uff0c\u77ed\u671f\u4fdd\u5b58\u8bf7\u7f6e\u4e8e-30 ~<\/li>\n<\/ol>\n

          -15\u2103 , \u957f\u671f\u4fdd\u5b58\u8bf7\u7f6e\u4e8e-85 ~ -65\u2103\u3002<\/p>\n

           <\/p>\n

           <\/p>\n

          9\/<\/strong>\u5e38\u89c1\u95ee\u9898\u4e0e\u89e3\u51b3\u65b9\u6848<\/p>\n

           <\/p>\n\n\n\n\n\n\n\n\n\n\n\n
          \u5e38\u89c1\u95ee\u9898<\/strong><\/td>\n\u539f\u56e0<\/strong><\/td>\n\u89e3\u51b3\u65b9\u6848<\/strong><\/td>\n<\/tr>\n
           <\/p>\n

           <\/p>\n

          \u672a\u63d0\u53d6\u5230<\/p>\n

          DNA\/RNA<\/strong>\u6216<\/p>\n

          \u4ea7\u91cf\u4f4e<\/td>\n

          		1.\u6837\u672c\u53cd\u590d\u51bb\u878d<\/td>\n\u4f7f\u7528\u65b0\u9c9c\u6837\u672c \uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d<\/td>\n<\/tr>\n
          2.\u6838\u9178\u4fdd\u62a4\u5242\u50a8\u5b58\u4e0d\u5f53<\/td>\n\u6838\u9178\u4fdd\u62a4\u5242\u5e94\u9884\u5148\u5206\u88c5\u540e\u7f6e\u4e8e-30 ~ -15\u2103\u4fdd\u5b58 \uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d<\/td>\n<\/tr>\n
          3.\u6d17\u8131\u4e0d\u5145\u5206<\/td>\nRNase-free ddH2O\u52a0\u81f3\u819c\u4e2d\u592e \uff0c\u9002\u5f53\u51cf\u5c11\u6d17\u8131\u4f53\u79ef \uff0c\u53ef\u4e8e65\u2103 \u9884\u70ed \uff0c\u5ef6\u957f\u9759\u7f6e\u65f6\u95f4\u6216\u8fdb\u884c\u4e8c\u6b21\u6d17\u8131<\/td>\n<\/tr>\n
          4.\u6837\u672c\u672a\u6062\u590d\u81f3\u5ba4\u6e29<\/td>\n\u6837\u672c\u5148\u6062\u590d\u81f3\u5ba4\u6e29 \uff0c\u518d\u8fdb\u884c\u6838\u9178\u63d0\u53d6<\/td>\n<\/tr>\n
          5.\u79bb\u5fc3\u6e29\u5ea6\u4e0d\u5f53<\/td>\n\u4f7f\u7528\u5ba4\u6e29\u6761\u4ef6\u79bb\u5fc3<\/td>\n<\/tr>\n
           <\/p>\n

           <\/p>\n

          \u4e0b\u6e38\u7ed3\u679c\u4e0d \u7406\u60f3<\/td>\n

          		1.\u76d0\u79bb\u5b50\u6b8b\u7559<\/td>\n\u6cbf\u5438\u9644\u67f1\u7ba1\u58c1\u56db\u5468\u52a0\u5165Buffer \u6d17\u8131\u6db22 \uff0c\u6216\u52a0\u5165Buffer \u6d17\u8131\u6db22 \u540e\u76d6\u76d6\u98a0\u5012\u6df7\u53002 – 3\u6b21 \uff0c\u6709\u52a9\u4e8e\u5b8c\u5168\u51b2\u6d17\u7ba1\u58c1\u4e0a\u7684\u76d0\u5206<\/td>\n<\/tr>\n
          2.\u4e59\u9187\u6b8b\u7559<\/td>\n\u7a7a\u67f1\u79bb\u5fc3\u540e \uff0c\u6253\u5f00\u5438\u9644\u67f1\u76d6\u5b50\u5ba4\u6e29\u9759\u7f6e5 min \uff0c\u6700\u5927\u7a0b\u5ea6\u53bb\u9664\u4e59 \u9187\u6b8b\u7559<\/td>\n<\/tr>\n
          3.\u8bd5\u5242\u51c6\u5907\u6709\u8bef<\/td>\n\u4f7f\u7528\u524d\u6309\u7167\u6807\u7b7e\u63d0\u793a \uff0c\u5206\u522b\u5411Buffer\u6d17\u8131\u6db21\u3001Buffer \u6d17\u8131\u6db22\u52a0 \u5165\u76f8\u5e94\u7684\u65e0\u6c34\u4e59\u9187<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

           <\/p>\n

           <\/p>\n","protected":false},"excerpt":{"rendered":"

          SPF-Level Mouse\/Rat Pathogen DNA\/RNA Kit Pro
          \nDNA\/RNA\u5171\u63d0\u53d6\u8bd5\u5242\u76d2 \u5171\u63d0\u53d6\u8bd5\u5242\u76d2\u4ea7\u54c1\u76ee\u5f55<\/p>\n","protected":false},"featured_media":408,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[19,41,25],"product_tag":[],"class_list":{"0":"post-418","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-cpzx","7":"product_cat-qtsjhxl","8":"product_cat-sydwsjh","10":"first","11":"instock","12":"shipping-taxable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/product\/418","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/comments?post=418"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/media\/408"}],"wp:attachment":[{"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/media?parent=418"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/product_brand?post=418"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/product_cat?post=418"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/product_tag?post=418"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}