{"id":421,"date":"2025-03-27T15:54:48","date_gmt":"2025-03-27T07:54:48","guid":{"rendered":"https:\/\/www.biowing.com.cn\/?post_type=product&#038;p=421"},"modified":"2025-06-09T12:25:50","modified_gmt":"2025-06-09T04:25:50","slug":"sydwsjhdxsxjbd6","status":"publish","type":"product","link":"https:\/\/www.biowing.com.cn\/index.php\/product\/sydwsjhdxsxjbd6\/","title":{"rendered":"\u5927\u5c0f\u9f20\u9009\u68c0\u75c5\u6bd2\u68c0\u6d4b\u8bd5\u5242\u76d2 \uff08\u8054\u68c0\/\u9274\u5b9a\uff0c6\u79cd\uff09"},"content":{"rendered":"<p>SPF\u7ea7\u5927\u5c0f\u9f20\u75c5\u539f\u4f53\u8d28\u91cf\u63a7\u5236\u591a\u91cdqPCR\u8054\u68c0\u8bd5\u5242\u76d2\uff08\u56fd\u6807\u75c5\u6bd2\u9009\u68c0\u516d\u9879\uff09<\/p>\n<p>\uff08\u63a2\u9488\u6cd5\uff09<\/p>\n<p>\u3010\u4ea7\u54c1\u89c4\u683c\u3011<\/p>\n<p>SPF.M\/R-OV-001 50 T\/\u76d2<\/p>\n<p>\u3010\u4ea7\u54c1\u8bf4\u660e\u3011<\/p>\n<p>Biowing<sup>\u00ae<\/sup>SPF\u7ea7\u5927\u5c0f\u9f20\u56fd\u6807\u9009\u68c0\u75c5\u6bd2qPCR\u8054\u68c0\u8bd5\u5242\u76d2\uff0c\u91c7\u7528Taqman\u8367\u5149\u5b9a\u91cfPCR\u6280\u672f\uff0c\u4e13\u4e3a\u5728\u591a\u79cd\u6837\u672c\u7c7b\u578b\u4e2d\u5feb\u901f\u3001\u9ad8\u7075\u654f\u5730\u7b5b\u67e5SPF\u7ea7\u5b9e\u9a8c\u5ba4\u5927\u5c0f\u9f20\u5e38\u89c1\u76846\u79cd\u75c5\u6bd2\u800c\u8bbe\u8ba1\u3002<\/p>\n<p>\u672c\u8bd5\u5242\u76d2\u91c7\u7528\u4e00\u6b65\u6cd5RT-qPCR\uff0c\u8bbe\u8ba16\u5957\u5f15\u7269\u63a2\u9488\u5206\u522b\u5728FAM\uff08\u6dcb\u5df4\u7ec6\u80de\u8109\u7edc\u4e1b\u8111\u819c\u708e\u75c5\u6bd2LCMV\uff08RNA\uff09\u3001\u5c0f\u9f20\u8bfa\u5982\u75c5\u6bd2MNV\uff08RNA\uff09\u3001\u5927\u9f20\u51a0\u72b6\u75c5\u6bd2RCV\uff08DNA\uff09\uff09\uff0cHEX\uff08\u9f20\u75d8\u75c5\u6bd2\/\u8131\u811a\u75c5\u6bd2Ect\uff08DNA\uff09\u3001\u5c0f\u9f20\u8111\u810a\u9ad3\u708e\u75c5\u6bd2TMEV\uff08RNA\uff09\u3001\u591a\u7624\u75c5\u6bd2Poly\uff08DNA\uff09\uff09\u4e24\u4e2a\u901a\u9053\u68c0\u6d4b6\u79cd\u75c5\u6bd2\uff0c\u53e6\u5916\u4e00\u5957\u5f15\u7269\u63a2\u9488\u5728CY5\u901a\u9053\u68c0\u6d4b\u5185\u53c2RNA\u3002\u8be5\u8bd5\u5242\u76d2\u5177\u6709\u4ee5\u4e0b\u7279\u70b9\uff1a<\/p>\n<p><strong>\u7075\u654f<\/strong>\uff1a\u68c0\u6d4b\u7075\u654f\u5ea6\u8f83\u9ad8\uff0c10 copies\/\u03bcL\u53ef\u88ab\u6709\u6548\u68c0\u51fa\u3002<\/p>\n<p><strong>\u9ad8\u6548<\/strong>\uff1a\u5355\u7ba1\u4e00\u6b21\u53ef\u68c0\u51fa6\u79cd\u75c5\u6bd2\u3002<\/p>\n<p><strong>\u7a33\u5b9a<\/strong>\uff1a\u5168\u7a0b\u95ed\u7ba1\u64cd\u4f5c\uff0c\u65e0\u4ea4\u53c9\u6c61\u67d3\u3002<\/p>\n<p><strong>\u53ef\u9760<\/strong>\uff1a\u542b\u5185\u53c2\u5bf9\u7167\uff0c\u907f\u514d\u5047\u9634\u6027\u3002<\/p>\n<p><strong>\u65b9\u4fbf<\/strong>\uff1a\u64cd\u4f5c\u7b80\u5355\uff0c\u4e00\u6b65\u6cd5\u68c0\u6d4b\u3002<\/p>\n<p>\u3010\u8fd0\u8f93\u53ca\u4fdd\u5b58\u6761\u4ef6\u3011<\/p>\n<p>\u4f4e\u6e29\u51b7\u51bb\u8fd0\u8f93\uff0c-20\u2103\u4fdd\u5b58\uff0c\u6709\u6548\u671f1\u5e74\uff1b\u4f7f\u7528\u540e\u4ecd\u5b58\u653e\u5728-20\u2103\uff0c\u4e0d\u53ef\u53cd\u590d\u51bb\u878d\u8d85\u8fc7\u4e09\u6b21\u3002<\/p>\n<p>\u3010\u4ea7\u54c1\u7ec4\u5206\u3011<\/p>\n<p>A BOX:<\/p>\n<table width=\"229\">\n<tbody>\n<tr>\n<td width=\"155\">\u7ec4\u5206\u540d\u79f0<\/td>\n<td width=\"74\">\u88c5\u91cf<\/td>\n<\/tr>\n<tr>\n<td width=\"155\">Biowing<sup>\u00ae<\/sup>Virus qPCR Reaction Mix<\/td>\n<td width=\"74\">110 \u03bcL\u00d72\u7ba1<\/td>\n<\/tr>\n<tr>\n<td width=\"155\">Biowing<sup>\u00ae<\/sup>Optional Virus Primer&amp;Probe Mix<\/td>\n<td width=\"74\">\u51bb\u5e72\u7c89\u00d72\u7ba1<\/td>\n<\/tr>\n<tr>\n<td width=\"155\">RNase Free Water<\/td>\n<td width=\"74\">1.1mL\u00d72\u7ba1<\/td>\n<\/tr>\n<tr>\n<td width=\"155\">\u77f3\u8721\u6cb9<\/td>\n<td width=\"74\">1.1mL\u00d71\u7ba1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>B BOX:<\/p>\n<table width=\"229\">\n<tbody>\n<tr>\n<td width=\"156\">\u7ec4\u5206\u540d\u79f0<\/td>\n<td width=\"73\">\u88c5\u91cf<\/td>\n<\/tr>\n<tr>\n<td width=\"156\">\u9633\u6027\u8d28\u63a71\uff08PC1\uff09<\/td>\n<td width=\"73\">\u51bb\u5e72\u7c89\u00d72\u7ba1<\/td>\n<\/tr>\n<tr>\n<td width=\"156\">\u9633\u6027\u8d28\u63a72\uff08PC2\uff09<\/td>\n<td width=\"73\">\u51bb\u5e72\u7c89\u00d72\u7ba1<\/td>\n<\/tr>\n<tr>\n<td width=\"156\">\u9633\u6027\u8d28\u63a73\uff08PC3\uff09<\/td>\n<td width=\"73\">\u51bb\u5e72\u7c89\u00d72\u7ba1<\/td>\n<\/tr>\n<tr>\n<td width=\"156\">RNase Free Water<\/td>\n<td width=\"73\">1.1 mL\u00d71\u7ba1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u6ce8\uff1a<\/strong><\/p>\n<p>\uff081\uff09\u9633\u6027\u8d28\u63a7\uff08PC1\uff09\u5305\u542b3\u79cd\u75c5\u6bd2\u6838\u9178\u6216\u542b\u7279\u5b9a\u7247\u6bb5\u7684\u8d28\u7c92DNA\uff0c\u5206\u522b\u662f\u6dcb\u5df4\u7ec6\u80de\u8109\u7edc\u4e1b\u8111\u819c\u708e\u75c5\u6bd2LCMV\u3001\u9f20\u75d8\u75c5\u6bd2\/\u8131\u811a\u75c5\u6bd2Ect\u3001\u5185\u53c2RNA\u3002<\/p>\n<p>\uff082\uff09\u9633\u6027\u8d28\u63a7\uff08PC2\uff09\u5305\u542b3\u79cd\u75c5\u6bd2\u6838\u9178\u6216\u542b\u7279\u5b9a\u7247\u6bb5\u7684\u8d28\u7c92DNA\uff0c\u5206\u522b\u662f\u5c0f\u9f20\u8bfa\u5982\u75c5\u6bd2MNV\u3001\u5c0f\u9f20\u8111\u810a\u9ad3\u708e\u75c5\u6bd2TMEV\u3001\u5185\u53c2RNA\u3002<\/p>\n<p>\uff083\uff09\u9633\u6027\u8d28\u63a7\uff08PC3\uff09\u5305\u542b3\u79cd\u75c5\u6bd2\u6838\u9178\u6216\u542b\u75c5\u6bd2\u7279\u5b9a\u7247\u6bb5\u7684\u8d28\u7c92DNA\uff0c\u5206\u522b\u662f\u5927\u9f20\u51a0\u72b6\u75c5\u6bd2RCV\u3001\u591a\u7624\u75c5\u6bd2Poly\u3001\u5185\u53c2RNA\u3002<\/p>\n<p>\u3010\u64cd\u4f5c\u6b65\u9aa4\u3011<\/p>\n<p>1. \u6837\u672c\u5236\u5907<\/p>\n<p>\u63a8\u8350\u914d\u5957\u4f7f\u7528\u7ffc\u548cSPF-Level Mouse\/Rat Pathgen DNA\/RNA Kit Pro\uff08\u8d27\u53f7\uff1aPRE.M\/R-001\uff09\uff0c\u6b64\u8bd5\u5242\u76d2\u63d0\u53d6\u5feb\u901f\uff0c\u56de\u6536\u7387\u8f83\u9ad8\uff0c\u5e76<strong>\u63d0\u4f9b\u6838\u9178\u4fdd\u62a4\u5242\uff08\u542b\u5185\u90e8\u8d28\u63a7\uff09\uff0c\u53ef\u4ee5\u5bf9\u63d0\u53d6\u8fc7\u7a0b\u8fdb\u884c\u8d28\u63a7<\/strong>\uff0c\u82e5\u4f7f\u7528\u5176\u4ed6\u63d0\u53d6\u8bd5\u5242\u76d2\u9700\u5148\u81ea\u884c\u6d4b\u8bd5\u3002<\/p>\n<p>2. \u4ea7\u54c1\u7ec4\u5206\u7684\u590d\u6eb6<\/p>\n<p>A BOX\uff1aBiowing<sup>\u00ae<\/sup>Optional Virus Primer&amp;Probe Mix\u514812,000 rpm\u79bb\u5fc35 min\uff0c\u6309\u4e0b\u8868\u52a0\u5165RNase Free Water\uff0c\u6da1\u65cb\u9707\u83611 min\uff0c\u77ac\u79bb5 s\uff0c\u5ba4\u6e29\u653e\u7f6e5-10 min\u540e\u5907\u7528\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"104\">A BOX\uff1a<\/td>\n<td width=\"135\">\u6bcf\u7ba1\u9700\u52a0\u5165RNase Free Water\u91cf<\/td>\n<\/tr>\n<tr>\n<td width=\"104\">Biowing<sup>\u00ae<\/sup>Optional Virus Primer&amp;Probe Mix<\/td>\n<td width=\"135\">302 \u03bcL<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\" width=\"239\">\u6ce8\uff1aA\u3001B BOX\u590d\u6eb6\u5206\u522b\u7528\u76d2\u4e2d\u6240\u7ed9\u7684RNase Free Water\uff0c\u8bf7\u52ff\u6df7\u7528\u3001\u590d\u7528\uff0c\u907f\u514d\u6c61\u67d3\uff1b\u4ea7\u54c1\u7ec4\u5206\u590d\u6eb6\u540e\u5efa\u8bae-20\u2103\u4fdd\u5b58\uff0c\u53cd\u590d\u51bb\u878d\u4e0d\u8d85\u8fc7\u4e09\u6b21\uff0c\u53ef\u5206\u88c5\u540e\u50a8\u5b58\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>B BOX\uff1a\u9633\u6027\u8d28\u63a71\uff08PC1\uff09\u3001\u9633\u6027\u8d28\u63a72\uff08PC2\uff09\u3001\u9633\u6027\u8d28\u63a73\uff08PC3\uff09\u514812,000 rpm\u79bb\u5fc35 min\uff0c\u6309\u4e0b\u8868\u52a0\u5165RNase Free Water\uff0c\u6da1\u65cb\u9707\u83611 min\uff0c\u77ac\u79bb5 s\uff0c\u5ba4\u6e29\u653e\u7f6e5-10 min\u540e\u5907\u7528\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"104\">B BOX\uff1a<\/td>\n<td width=\"135\">\u6bcf\u7ba1\u9700\u52a0\u5165RNase Free Water\u91cf<\/td>\n<\/tr>\n<tr>\n<td width=\"104\">\u9633\u6027\u8d28\u63a71\uff08PC1\uff09<\/td>\n<td width=\"135\">44 \u03bcL<\/td>\n<\/tr>\n<tr>\n<td width=\"104\">\u9633\u6027\u8d28\u63a72\uff08PC2\uff09<\/td>\n<td width=\"135\">44 \u03bcL<\/td>\n<\/tr>\n<tr>\n<td width=\"104\">\u9633\u6027\u8d28\u63a73\uff08PC3\uff09<\/td>\n<td width=\"135\">44 \u03bcL<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\" width=\"239\">\u6ce8\uff1aA\u3001B BOX\u590d\u6eb6\u5206\u522b\u7528\u76d2\u4e2d\u6240\u7ed9\u7684RNase Free Water\uff0c\u8bf7\u52ff\u6df7\u7528\u3001\u590d\u7528\uff0c\u907f\u514d\u6c61\u67d3\uff1b\u9633\u6027\u8d28\u63a7\u52a1\u5fc5\u4e0eA BOX\u5206\u5f00\u4fdd\u5b58\uff0c\u9632\u6b62\u6c61\u67d3\uff0c\u590d\u6eb6\u540e\u7acb\u5373\u901a\u98ce\u5e76\u55b7\u6d12\u9152\u7cbe\uff0c\u4ee5\u9632\u6c14\u6eb6\u80f6\u6c61\u67d3\uff1b\u4ea7\u54c1\u7ec4\u5206\u590d\u6eb6\u540e\u5efa\u8bae-20\u2103\u4fdd\u5b58\uff0c\u53cd\u590d\u51bb\u878d\u4e0d\u8d85\u8fc7\u4e09\u6b21\uff0c\u53ef\u5206\u88c5\u540e\u50a8\u5b58\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>3. qPCR \u53cd\u5e94\u6db2\u7684\u51c6\u5907<\/p>\n<p>\uff081\uff09\u6839\u636e\u6240\u8981\u68c0\u6d4b\u6837\u54c1\u7684\u6570\u91cf\uff0c\u8ba1\u7b97\u6240\u9700\u53cd\u5e94\u5b54\u6570\uff0c\u6bcf\u4e2a\u6837\u672c\u505a\u5355\u5b54\u3002<\/p>\n<p>\u53cd\u5e94\u5b54\u6570=3\u4e2a\u9633\u6027\u8d28\u63a7+1\u4e2a\u9634\u6027\u8d28\u63a7+\u6837\u672c\u6570<\/p>\n<p>\uff082\uff09\u6839\u636e\u53cd\u5e94\u5b54\u6570\u8ba1\u7b97\u6240\u9700\u7684Biowing<sup>\u00ae<\/sup>Virus Probe qPCR Mix\u603b\u91cf\uff08\u9700\u67091\u5b54\u7684\u52a0\u6837\u635f\u5931\u91cf\uff09\uff1a<\/p>\n<p>Biowing<sup>\u00ae<\/sup>Virus Probe qPCR Mix=\uff08\u53cd\u5e94\u5b54\u6570+1\uff09\u00d715 \u03bcL<\/p>\n<p>\uff083\uff09\u5404\u8bd5\u5242\u653e\u5ba4\u6e29\u878d\u5316\uff0c\u5e76\u975e\u6839\u636e\u4e0b\u8868\u6240\u793a\u51c6\u5907qPCR mix\uff1a<\/p>\n<table width=\"239\">\n<tbody>\n<tr>\n<td width=\"54\">\u7ec4\u5206\/\u6837\u54c1\u7ba1<\/td>\n<td width=\"68\">Biowing<sup>\u00ae<\/sup>Virus\u00a0 qPCR Reaction Mix (\u00b5L)<\/td>\n<td width=\"81\">Biowing<sup>\u00ae<\/sup>Optional Virus Primer&amp;Probe Mix<\/p>\n<p>(\u00b5L)<\/td>\n<td width=\"35\">\u603b\u4f53\u79ef<\/p>\n<p>(\u00b5L)<\/td>\n<\/tr>\n<tr>\n<td width=\"54\">1\u5b54<\/td>\n<td width=\"68\">4<\/td>\n<td width=\"81\">11<\/td>\n<td width=\"35\">15<\/td>\n<\/tr>\n<tr>\n<td width=\"54\">N\u5b54<\/p>\n<p>\uff08\u53cd\u5e94\u5b54\u6570+1\uff09<\/td>\n<td width=\"68\">4N<\/td>\n<td width=\"81\">11N<\/td>\n<td width=\"35\">15N<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>4. qPCR \u53cd\u5e94\u4f53\u7cfb\u53ca\u53cd\u5e94\u6761\u4ef6<\/p>\n<p>\uff081\uff09\u9707\u8361\u6df7\u5300Biowing<sup>\u00ae<\/sup>Virus Probe qPCR Mix\uff0c\u4f4e\u901f\u79bb\u5fc3\uff0c\u5c06\u7ba1\u76d6\u6b8b\u7559\u6db2\u4f53\u6536\u96c6\u81f3\u7ba1\u5e95\u3002<\/p>\n<p>\uff082\uff09\u5411\u6bcf\u5b54\u53cd\u5e94\u7ba1\u4e2d\u5206\u88c515 \u03bcL qPCR Mix\u3002<\/p>\n<p>\uff083\uff09\u5411\u5df2\u5206\u88c5\u8fc7qPCR Mix\u7684\u53cd\u5e94\u7ba1\u4e2d\u52a0\u516515 \u03bcL\u77f3\u8721\u6cb9\u3002<\/p>\n<p>\uff084\uff09\u5411\u53cd\u5e94\u7ba1\u4e2d\u52a0\u5165\u6837\u54c1\uff0c3,000 rpm \u77ac\u79bb5 s\uff0c\u52a0\u6837\u793a\u4f8b\u5982\u4e0b\uff1a<\/p>\n<table width=\"247\">\n<tbody>\n<tr>\n<td width=\"48\">\u7ec4\u5206\/\u6837\u54c1\u7ba1<\/td>\n<td width=\"36\">\u5f85\u6d4b\u6837\u54c1\u7ba1<\/p>\n<p>(<strong>\u00b5<\/strong>L)<\/td>\n<td width=\"42\">\u9633\u6027\u5bf9\u7167\u7ba11<\/p>\n<p>(<strong>\u00b5<\/strong>L)<\/td>\n<td width=\"44\">\u9633\u6027\u5bf9\u7167\u7ba12<\/p>\n<p>(\u00b5L)<\/td>\n<td width=\"43\">\u9633\u6027\u5bf9\u7167\u7ba13<\/p>\n<p>(\u00b5L)<\/td>\n<td width=\"35\">\u9634\u6027\u5bf9\u7167\u7ba1<\/p>\n<p>(<strong>\u00b5<\/strong>L)<\/td>\n<\/tr>\n<tr>\n<td width=\"48\">Biowing<sup>\u00ae<\/sup>Virus Probe qPCR Mix<\/td>\n<td width=\"36\">15<\/td>\n<td width=\"42\">15<\/td>\n<td width=\"44\">15<\/td>\n<td width=\"43\">15<\/td>\n<td width=\"35\">15<\/td>\n<\/tr>\n<tr>\n<td width=\"48\">RNase Free Water<\/td>\n<td width=\"36\">0<\/td>\n<td width=\"42\">0<\/td>\n<td width=\"44\">0<\/td>\n<td width=\"43\">0<\/td>\n<td width=\"35\">5<\/td>\n<\/tr>\n<tr>\n<td width=\"48\">Sample<\/td>\n<td width=\"36\">5<\/td>\n<td width=\"42\">0<\/td>\n<td width=\"44\">0<\/td>\n<td width=\"43\">0<\/td>\n<td width=\"35\">0<\/td>\n<\/tr>\n<tr>\n<td width=\"48\">\u9633\u6027\u8d28\u63a71\uff08PC1\uff09<\/td>\n<td width=\"36\">0<\/td>\n<td width=\"42\">5<\/td>\n<td width=\"44\">0<\/td>\n<td width=\"43\">0<\/td>\n<td width=\"35\">0<\/td>\n<\/tr>\n<tr>\n<td width=\"48\">\u9633\u6027\u8d28\u63a72\uff08PC2\uff09<\/td>\n<td width=\"36\">0<\/td>\n<td width=\"42\">0<\/td>\n<td width=\"44\">5<\/td>\n<td width=\"43\">0<\/td>\n<td width=\"35\">0<\/td>\n<\/tr>\n<tr>\n<td width=\"48\">\u9633\u6027\u8d28\u63a73\uff08PC3\uff09<\/td>\n<td width=\"36\">0<\/td>\n<td width=\"42\">0<\/td>\n<td width=\"44\">0<\/td>\n<td width=\"43\">5<\/td>\n<td width=\"35\">0<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>PCR<\/strong><strong>\u4eea\u8bbe\u7f6e\u4ee5\u5b8f\u77f3<\/strong><strong>SLAN-96S<\/strong><strong>\u4e3a\u4f8b\uff0c\u5148\u65b0\u5efa\u5e76\u4fdd\u5b58\u7a0b\u5e8f\u9879\u76ee\uff0c\u9009\u62e9\u5b9a\u6027<\/strong><strong>\/<\/strong><strong>\u7edd\u5bf9\u5b9a\u91cf\u6a21\u5f0f\uff0c\u53cd\u5e94\u4f53\u7cfb<\/strong><strong>20 \u00b5L<\/strong><strong>\uff0c\u9009\u62e9<\/strong><strong>FAM<\/strong><strong>\u3001<\/strong><strong>HEX<\/strong><strong>\u3001<\/strong><strong>CY5<\/strong><strong>\u901a\u9053\uff0c\u6309\u7167\u4e0b\u8868\u8bbe\u7f6e\u7a0b\u5e8f\u8fdb\u884c\u4fdd\u5b58\uff1a<\/strong><\/p>\n<table width=\"236\">\n<tbody>\n<tr>\n<td colspan=\"2\" width=\"68\">\u6b65\u9aa4<\/td>\n<td width=\"44\">\u5faa\u73af\u6570<\/td>\n<td width=\"62\">\u6e29\u5ea6<\/td>\n<td width=\"62\">\u65f6\u95f4<\/td>\n<\/tr>\n<tr>\n<td width=\"16\">1<\/td>\n<td width=\"52\">\u9006\u8f6c\u5f55<\/td>\n<td width=\"44\">1<\/td>\n<td width=\"62\">55\u2103<\/td>\n<td width=\"62\">15 min<\/td>\n<\/tr>\n<tr>\n<td width=\"16\">2<\/td>\n<td width=\"52\">\u9884\u53d8\u6027<\/td>\n<td width=\"44\">1<\/td>\n<td width=\"62\">95\u2103<\/td>\n<td width=\"62\">30 s<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"2\" width=\"16\">3<\/td>\n<td width=\"52\">\u53d8\u6027<\/td>\n<td rowspan=\"2\" width=\"44\">40<\/td>\n<td width=\"62\">95\u2103<\/td>\n<td width=\"62\">10 s<\/td>\n<\/tr>\n<tr>\n<td width=\"52\">\u9000\u706b\u4e0e\u5ef6\u4f38<\/td>\n<td width=\"62\">60\u2103<\/td>\n<td width=\"62\">30 s<\/td>\n<\/tr>\n<tr>\n<td colspan=\"5\" width=\"236\">60\u2103\u5ef6\u4f38\u65f6\u6536\u96c6\u8367\u5149\u4fe1\u53f7<\/td>\n<\/tr>\n<tr>\n<td colspan=\"5\" width=\"236\">\u901a\u9053\u9009\u62e9\uff1a\u6837\u672c\u68c0\u6d4b\u901a\u9053-FAM\u3001HEX\uff1b\u5185\u53c2\u68c0\u6d4b\u901a\u9053-CY5\u3002\u5b8f\u77f3SLAN-96S\u8367\u5149\u5b9a\u91cf PCR \u4eea\u4e0d\u9700 ROX \u6821\u51c6\u3002\u82e5\u5b58\u5728\u90e8\u5206\u8367\u5149\u5b9a\u91cfPCR\u4eea\u65e0CY5\u901a\u9053\uff0c\u5373\u4e0d\u5206\u6790\u8be5\u901a\u9053\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u6ce8\uff1a\u53cd\u5e94\u70ed\u7a0b\u5e8fDNA\/RNA\u7686\u9002\u7528\u3002<\/p>\n<p>\u3010\u9608\u503c\u8bbe\u7f6e\u3011<\/p>\n<p>\u4ee5\u5b8f\u77f3SLAN-96S\u4e3a\u4f8b\uff0c\u5e38\u7528\u9009\u9879\u4e2d\u6269\u589e\u66f2\u7ebf\u7b97\u6cd5\u9009\u62e9\u76f8\u5bf9\u8367\u5149\u503c\u6cd5-log\uff0c\u57fa\u7ebf\u8bbe\u7f6e3-15\u4e2a\u5faa\u73af\u3002<\/p>\n<p>\uff081\uff09\u6309\u7167\u673a\u5668\u7684\u81ea\u52a8\u9608\u503c\u6216\u8005\u624b\u52a8\u8c03\u6574\u9608\u503c\u7ebf\u5c06\u9633\u6027\u8d28\u63a7\uff08PC\uff09\u5404\u901a\u9053\u7684Ct\u503c\u5b9a\u4e3a\u226432\uff0c\u6839\u636e\u8be5\u9608\u503c\u7ebf\u8fdb\u884c\u68c0\u6d4b\u6837\u672c\u7ed3\u679c\u7684\u5224\u5b9a\u3002<\/p>\n<p>\u3010\u7ed3\u679c\u5224\u8bfb\u3011<\/p>\n<p>\u4ee5\u5b8f\u77f3SLAN-96S\u4e3a\u4f8b\uff0c\u6839\u636e\u6837\u672c\uff08FAM\/HEX\uff09Ct\u503c\u5224\u5b9a\u662f\u5426\u53d1\u751f\u75c5\u539f\u83cc\u611f\u67d3\uff0c\u5177\u4f53\u6807\u51c6\u5982\u4e0b\uff1a<\/p>\n<table width=\"233\">\n<tbody>\n<tr>\n<td width=\"55\">PCR\u6a21\u677f<\/td>\n<td width=\"124\">\u6837\u672c\uff08FAM\/HEX\uff09<\/td>\n<td width=\"54\">\u5224\u5b9a\u8bf4\u660e<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"2\" width=\"55\">\u9633\u6027\u5bf9\u7167<\/p>\n<p>\uff081\u30012\u30013\uff09<\/td>\n<td width=\"124\">\u226432<\/td>\n<td width=\"54\">\u9633\u6027\u6210\u7acb<\/td>\n<\/tr>\n<tr>\n<td width=\"124\">\uff1e32<\/td>\n<td width=\"54\">\u9633\u6027\u4e0d\u6210\u7acb<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"2\" width=\"55\">\u5f85\u6d4b\u6837\u54c1<\/td>\n<td width=\"124\">\uff1e40<\/td>\n<td width=\"54\">\u9634\u6027\u6210\u7acb<\/td>\n<\/tr>\n<tr>\n<td width=\"124\">\u226440<\/td>\n<td width=\"54\">\u9633\u6027\u6210\u7acb<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u6ce8\uff1a<\/strong><\/p>\n<ol>\n<li>\u6837\u672c\u9633\u6027\u5224\u65ad\u6807\u51c6\u4e3a\uff1a<\/li>\n<\/ol>\n<p>\uff081\uff09\u6837\u672cCt\u503c\u226440\u65f6\uff0c\u5224\u5b9a\u4e3a\u9633\u6027\u3002\u5176\u4e2dFAM\u901a\u9053Ct\u503c\u226440\uff0c\u6837\u672c\u53ef\u80fd\u611f\u67d3\u6dcb\u5df4\u7ec6\u80de\u8109\u7edc\u4e1b\u8111\u819c\u708e\u75c5\u6bd2LCMV\u3001\u5c0f\u9f20\u8bfa\u5982\u75c5\u6bd2MNV\u3001\u5927\u9f20\u51a0\u72b6\u75c5\u6bd2RCV\u4e2d\u7684\u4e00\u79cd\u6216\u51e0\u79cd\uff1bHEX\u901a\u9053Ct\u503c\u226440\uff0c\u6837\u672c\u53ef\u80fd\u611f\u67d3\u9f20\u75d8\u75c5\u6bd2\/\u8131\u811a\u75c5\u6bd2Ect\u3001\u5c0f\u9f20\u8111\u810a\u9ad3\u708e\u75c5\u6bd2TMEV\u3001\u591a\u7624\u75c5\u6bd2Poly\u4e2d\u7684\u4e00\u79cd\u6216\u51e0\u79cd\u3002<\/p>\n<p>\uff082\uff09\u4e0d\u540c\u673a\u5668\u7684\u68c0\u6d4b\u7075\u654f\u5ea6\u4e0d\u540c\uff0c\u4e0d\u540c\u6837\u672c\u7c7b\u578b\u6027\u8d28\u4e0d\u540c\uff0c\u5efa\u8bae\u4f7f\u7528\u8be5\u7cfb\u5217\u8bd5\u5242\u76d2\u68c0\u6d4b\u6837\u672c\u524d\uff0c\u5229\u7528SPF\u6837\u672c\u8fdb\u884c\u9002\u5e94\u6027\u9a8c\u8bc1\u540e\u5236\u5b9a\u9633\u6027\u5224\u65ad\u6807\u51c6\u3002<\/p>\n<ol start=\"2\">\n<li>\u82e5\u5404\u901a\u9053\u6709\u9633\u6027\u51fa\u73b0\uff0c\u5efa\u8bae\u8fdb\u4e00\u6b65\u4f7f\u7528\u9274\u5b9a\u8bd5\u5242\u76d2\u8fdb\u884c\u5355\u4e00\u75c5\u539f\u4f53\u611f\u67d3\u786e\u8ba4\uff1a<\/li>\n<\/ol>\n<p>\uff081\uff09FAM\u901a\u9053\u9633\u6027\uff1a\u4f7f\u7528SPF.M\/R-OVF-004\u8bd5\u5242\u76d2\uff0c\u5bf9\u4e09\u79cd\u53ef\u80fd\u611f\u67d3\u7684\u75c5\u6bd2\uff08\u6dcb\u5df4\u7ec6\u80de\u8109\u7edc\u4e1b\u8111\u819c\u708e\u75c5\u6bd2LCMV\u3001\u5c0f\u9f20\u8bfa\u5982\u75c5\u6bd2MNV\u3001\u5927\u9f20\u51a0\u72b6\u75c5\u6bd2RCV\uff09\u8fdb\u884c\u786e\u8ba4\u3002<\/p>\n<p>\uff082\uff09HEX\u901a\u9053\u9633\u6027\uff1a\u4f7f\u7528SPF.M\/R-OVH-005\u8bd5\u5242\u76d2\uff0c\u5bf9\u4e09\u79cd\u53ef\u80fd\u611f\u67d3\u7684\u75c5\u6bd2\u9f20\u75d8\u75c5\u6bd2\/\u8131\u811a\u75c5\u6bd2Ect\u3001\u5c0f\u9f20\u8111\u810a\u9ad3\u708e\u75c5\u6bd2TMEV\u3001\u591a\u7624\u75c5\u6bd2Poly\u8fdb\u884c\u786e\u8ba4\u3002<\/p>\n<ol start=\"3\">\n<li>\u68c0\u67e5\u5185\u53c2\u901a\u9053\u6269\u589e\u662f\u5426\u6b63\u5e38\uff0c\u5185\u53c2\u6269\u589e\u4e0d\u6b63\u5e38\uff0c\u8868\u660e\u6838\u9178\u62bd\u63d0\u8fc7\u7a0b\u6709\u8bef\u6216\u8005PCR\u53cd\u5e94\u672a\u6b63\u5e38\u8fdb\u884c\uff0c\u9700\u91cd\u590d\u68c0\u6d4b\u3002<\/li>\n<\/ol>\n<p>\u3010\u6ce8\u610f\u4e8b\u9879\u3011<\/p>\n<p>\u7531\u4e8ePCR\u53cd\u5e94\u975e\u5e38\u7075\u654f\uff0c\u5b9e\u9a8c\u64cd\u4f5c\u4e2d\u5e94\u6ce8\u610f\u4ee5\u4e0b\u4e8b\u9879\uff0c\u4ee5\u514d\u53d1\u751f\u6838\u9178\u6c61\u67d3\u3002<\/p>\n<ol>\n<li><strong>\u5efa\u8bae\u5206\u533a\u64cd\u4f5c<\/strong><\/li>\n<\/ol>\n<p>\uff081\uff09A\u533a\uff1a\uff08\u6d01\u51c0\u533a\uff09\u914d\u5236Mix\u548cqPCR\u9634\u6027\u52a0\u6837\uff0c\u5efa\u8bae\u5728\u8d85\u51c0\u5de5\u4f5c\u53f0\u5b8c\u6210\uff0c\u5e76\u914d\u5957\u5e72\u51c0\u5668\u5177\u53ca\u65e0\u83cc\u65e0\u9176\u8017\u6750\u4e13\u7528\u4e8e\u4f53\u7cfb\u914d\u5236\u3002<\/p>\n<p>\uff082\uff09B\u533a\uff1a\uff08\u6838\u9178\u63d0\u53d6\u53ca\u52a0\u6837\u533a\uff09\u6837\u672cDNA\/RNA\u63d0\u53d6\uff0c\u5efa\u8bae\u5728\u8d85\u51c0\u5de5\u4f5c\u53f0\u5b8c\u6210\uff0c\u82e5\u65e0\u8d85\u51c0\u5de5\u4f5c\u53f0\u8bf7\u901a\u98ce\u72b6\u6001\u4e0b\u63d0\u53d6\u5e76\u7acb\u5373\u6d88\u6740\uff0c\u9700\u914d\u5957\u4e13\u7528\u4e8e\u6838\u9178\u63d0\u53d6\u7684\u5668\u5177\uff0c\u5e76\u5728\u6bcf\u6b21\u63d0\u53d6\u540e\u5bf9\u73af\u5883\u548c\u5668\u5177\u8fdb\u884c\u6838\u9178\u6d88\u6740\u5904\u7406\uff1b\u52a0\u6837\u533a\uff0c\u6309\u7167\u5148\u52a0\u5f85\u6d4b\u6837\u672c\uff0c\u540e\u52a0\u9633\u6027\u5bf9\u7167\u7684\u987a\u5e8f\u52a0\u6837\uff0c\u5728qPCR\u53cd\u5e94\u677f\u4e0a\u9633\u6027\u5bf9\u7167\u7684\u6392\u5e03\u5e94\u8fdc\u79bb\u5f85\u6d4b\u6837\u672c\u5b54\u548c\u9634\u6027\u5bf9\u7167\uff1b\u540c\u6837\u9700\u914d\u5957\u4e13\u7528\u4e8e\u52a0\u6837\u7684\u4eea\u5668\uff0c\u5e76\u5728\u6bcf\u6b21\u52a0\u5b8c\u6837\u540e\u8fdb\u884c\u6838\u9178\u6d88\u6740\u5904\u7406\u3002<\/p>\n<p>\uff083\uff09C\u533a\uff1a\uff08\u6838\u9178\u6269\u589e\u533a\uff09\u6b64\u533a\u57df\u653e\u7f6e\u8367\u5149\u5b9a\u91cfPCR\u4eea\u53ca\u76f8\u5173\u7535\u8111\u8fdb\u884c\u6570\u636e\u5904\u7406\uff0c\u540c\u6837\u9700\u5b9a\u671f\u8fdb\u884c\u6838\u9178\u6d88\u6740\u3002<\/p>\n<ol start=\"2\">\n<li><strong>\u5176\u4ed6<\/strong><\/li>\n<\/ol>\n<p>\uff081\uff09\u7531\u4e8eB BOX\u542b\u9633\u6027\u8d28\u63a7\uff0c\u4e3a\u907f\u514d\u8bd5\u5242\u53d7\u5230\u6838\u9178\u6c61\u67d3\uff0cA\u3001B BOX\u8bf7\u5206\u5f00\u50a8\u5b58\u3002<\/p>\n<p>\uff082\uff09\u6bcf\u4e2a\u7ec4\u5206\u5728\u4f7f\u7528\u524d\u90fd\u5e94\u5148\u4f4e\u901f\u79bb\u5fc3\u540e\u5c0f\u5fc3\u5f00\u542f\u3002\u4f7f\u7528\u65f6\u8bf7\u4e0a\u4e0b\u8f7b\u67d4\u98a0\u5012\u5341\u6b21\u6df7\u5300\uff0c\u907f\u514d\u8d77\u6ce1\uff0c\u5e76\u4f4e\u901f\u77ed\u6682\u79bb\u5fc3\u540e\u4f7f\u7528\u3002<\/p>\n<p>\uff083\uff09qPCR\u53cd\u5e94\u677f\u5c01\u819c\u6216\u76d6\u76d6\u5b50\u65f6\u9700\u53cd\u590d\u538b\u7d27\u3002<\/p>\n<p>\uff084\uff09\u4e0a\u673a\u6269\u589e\u524d\uff0c\u53cd\u5e94\u7ba1\u77ed\u65f6\u4f4e\u901f\u79bb\u5fc3\uff0c\u5c06\u7ba1\u58c1\u6db2\u4f53\u6536\u96c6\u81f3\u7ba1\u5e95\u3002<\/p>\n<p>\uff085\uff09\u76d6\u4e0a\u53cd\u5e94\u7ba1\u76d6\u5b50\u6216\u8005\u8d34\u4e0a\u5149\u5b66\u819c\u540e\uff0c\u4e3a\u907f\u514d\u5f71\u54cd\u8367\u5149\u4fe1\u53f7\u8bfb\u53d6\uff0c\u8bf7\u6ce8\u610f\u4e0d\u8981\u5728\u7ba1\u76d6\u6216\u8005\u819c\u4e0a\u505a\u6807\u8bb0\uff0c\u6216\u8005\u7528\u522e\u677f\u53cd\u590d\u6469\u64e6\u3002<\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u6709\u6280\u672f\u95ee\u9898\uff0c\u6b22\u8fce\u7535\u8bdd\u54a8\u8be2\uff1a<\/em><\/strong><strong><em>021-33559491<\/em><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>SPF\u7ea7\u5927\u5c0f\u9f20\u75c5\u539f\u4f53\u8d28\u91cf\u63a7\u5236\u591a\u91cdqPCR\u8054\u68c0\u8bd5\u5242\u76d2 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