{"id":424,"date":"2025-03-27T15:58:35","date_gmt":"2025-03-27T07:58:35","guid":{"rendered":"https:\/\/www.biowing.com.cn\/?post_type=product&#038;p=424"},"modified":"2025-08-08T15:45:58","modified_gmt":"2025-08-08T07:45:58","slug":"sydwsjhdxsxjbyj7","status":"publish","type":"product","link":"https:\/\/www.biowing.com.cn\/index.php\/product\/sydwsjhdxsxjbyj7\/","title":{"rendered":"\u5927\u5c0f\u9f20\u9009\u68c0\u75c5\u539f\u83cc\u68c0\u6d4b\u8bd5\u5242\u76d2 \uff08\u8054\u68c0\/\u9274\u5b9a\uff0c7\u79cd\uff09"},"content":{"rendered":"<div style=\"width: 1920px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-424-1\" width=\"1920\" height=\"1080\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/www.biowing.com.cn\/wp-content\/uploads\/689_1754631702.mp4?_=1\" \/><a href=\"https:\/\/www.biowing.com.cn\/wp-content\/uploads\/689_1754631702.mp4\">https:\/\/www.biowing.com.cn\/wp-content\/uploads\/689_1754631702.mp4<\/a><\/video><\/div>\n<p>SPF\u7ea7\u5927\u5c0f\u9f20\u75c5\u539f\u4f53\u8d28\u91cf\u63a7\u5236\u591a\u91cdqPCR\u8054\u68c0\u8bd5\u5242\u76d2\uff08\u56fd\u6807\u75c5\u539f\u83cc\u9009\u68c0\u4e03\u9879\uff09<\/p>\n<p>\uff08\u63a2\u9488\u6cd5\uff09<\/p>\n<p>\u3010\u4ea7\u54c1\u89c4\u683c\u3011<\/p>\n<p>SPF.M\/R-OB-001 50 T\/\u76d2<\/p>\n<p>\u3010\u4ea7\u54c1\u8bf4\u660e\u3011<\/p>\n<p>Biowing<sup>\u00ae<\/sup>SPF\u7ea7\u5927\u5c0f\u9f20\u56fd\u6807\u9009\u68c0\u75c5\u539f\u83ccqPCR\u8054\u68c0\u8bd5\u5242\u76d2\uff0c\u91c7\u7528\u9ad8\u7075\u654f\u5ea6\u548c\u7279\u5f02\u6027\u7684\u63a2\u9488\u6cd5qPCR\u6280\u672f\uff0c\u4e13\u4e3a\u5728\u591a\u79cd\u6837\u672c\u7c7b\u578b\u4e2d\u5feb\u901f\u3001\u9ad8\u7075\u654f\u5730\u7b5b\u67e5SPF\u7ea7\u5b9e\u9a8c\u5ba4\u5927\u5c0f\u9f20\u5e38\u89c1\u76847\u79cd\u75c5\u539f\u83cc\u800c\u8bbe\u8ba1\u3002<\/p>\n<p>\u672c\u8bd5\u5242\u76d2\u91c7\u7528Taqman\u8367\u5149\u5b9a\u91cfPCR\u6280\u672f\uff0c\u8bbe\u8ba17\u5957\u5f15\u7269\u63a2\u9488\u5206\u522b\u5728FAM\uff08\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83ccSa\u3001\u03b2-\u6eb6\u8840\u6027\u94fe\u7403\u83cc\u5c5eBHS\u3001\u725b\u68d2\u72b6\u6746\u83ccCb\uff09\uff0cHEX\uff08\u5ff5\u73e0\u72b6\u94fe\u6746\u83ccSm\u3001\u67e0\u6aac\u9178\u6746\u83ccCr\u3001\u80ba\u5b62\u5b50\u866bPs\uff09\uff0cROX\uff08\u80ba\u708e\u94fe\u7403\u83ccSp\uff09\u4e09\u4e2a\u901a\u9053\u68c0\u6d4b7\u79cd\u75c5\u539f\u83cc\uff0c\u53e6\u5916\u4e00\u5957\u5f15\u7269\u63a2\u9488\u5728CY5\u901a\u9053\u68c0\u6d4b\u5185\u53c2DNA\u3002\u8be5\u8bd5\u5242\u76d2\u5177\u6709\u4ee5\u4e0b\u7279\u70b9\uff1a<\/p>\n<p><strong>\u7075\u654f\uff1a<\/strong>\u68c0\u6d4b\u7075\u654f\u5ea6\u8f83\u9ad8\uff0c10 CFU\u53ef\u88ab\u6709\u6548\u68c0\u51fa\u3002<\/p>\n<p><strong>\u9ad8\u6548\uff1a<\/strong>\u5355\u7ba1\u4e00\u6b21\u53ef\u7b5b\u67e57\u79cd\u75c5\u539f\u83cc\u3002<\/p>\n<p><strong>\u7a33\u5b9a\uff1a<\/strong>\u5168\u7a0b\u95ed\u7ba1\u64cd\u4f5c\uff0c\u65e0\u4ea4\u53c9\u6c61\u67d3\u3002<\/p>\n<p><strong>\u53ef\u9760\uff1a<\/strong>\u542b\u5185\u53c2\u57fa\u56e0\uff0c\u907f\u514d\u5047\u9634\u6027\u3002<\/p>\n<p><strong>\u65b9\u4fbf\uff1a<\/strong>\u64cd\u4f5c\u7b80\u5355\uff0c\u4e00\u6b65\u6cd5\u68c0\u6d4b\u3002<\/p>\n<p>\u3010\u8fd0\u8f93\u53ca\u4fdd\u5b58\u6761\u4ef6\u3011<\/p>\n<p>\u4f4e\u6e29\u51b7\u51bb\u8fd0\u8f93\uff0c-20\u2103\u4fdd\u5b58\uff0c\u6709\u6548\u671f1\u5e74\uff1b\u4f7f\u7528\u540e\u4ecd\u5b58\u653e\u5728-20\u2103\uff0c\u4e0d\u53ef\u53cd\u590d\u51bb\u878d\u8d85\u8fc7\u4e09\u6b21\u3002<\/p>\n<p>\u3010\u4ea7\u54c1\u7ec4\u5206\u3011<\/p>\n<p>A BOX:<\/p>\n<table width=\"229\">\n<tbody>\n<tr>\n<td width=\"153\">\u7ec4\u5206\u540d\u79f0<\/td>\n<td width=\"76\">\u88c5\u91cf<\/td>\n<\/tr>\n<tr>\n<td width=\"153\">Biowing<sup>\u00ae<\/sup>Bacteria qPCR Reaction Mix<\/td>\n<td width=\"76\">275 \u03bcL\u00d72\u7ba1<\/td>\n<\/tr>\n<tr>\n<td width=\"153\">Biowing<sup>\u00ae<\/sup>Optional Bacteria Primer&amp;Probe Mix<\/td>\n<td width=\"76\">\u51bb\u5e72\u7c89\u00d72\u7ba1<\/td>\n<\/tr>\n<tr>\n<td width=\"153\">RNase Free Water<\/td>\n<td width=\"76\">1.1 mL\u00d72\u7ba1<\/td>\n<\/tr>\n<tr>\n<td width=\"153\">\u77f3\u8721\u6cb9<\/td>\n<td width=\"76\">1.1 mL\u00d71\u7ba1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>B BOX:<\/p>\n<table width=\"229\">\n<tbody>\n<tr>\n<td width=\"154\">\u7ec4\u5206\u540d\u79f0<\/td>\n<td width=\"75\">\u88c5\u91cf<\/td>\n<\/tr>\n<tr>\n<td width=\"154\">\u9633\u6027\u8d28\u63a71\uff08PC1\uff09<\/td>\n<td width=\"75\">\u51bb\u5e72\u7c89\u00d72\u7ba1<\/td>\n<\/tr>\n<tr>\n<td width=\"154\">\u9633\u6027\u8d28\u63a72\uff08PC2\uff09<\/td>\n<td width=\"75\">\u51bb\u5e72\u7c89\u00d72\u7ba1<\/td>\n<\/tr>\n<tr>\n<td width=\"154\">\u9633\u6027\u8d28\u63a73\uff08PC3\uff09<\/td>\n<td width=\"75\">\u51bb\u5e72\u7c89\u00d72\u7ba1<\/td>\n<\/tr>\n<tr>\n<td width=\"154\">RNase Free Water<\/td>\n<td width=\"75\">1.1 mL\u00d71\u7ba1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u6ce8\uff1a<\/strong><\/p>\n<p>\uff081\uff09\u9633\u6027\u8d28\u63a7\uff08PC1\uff09\u5305\u542b4\u79cd\u75c5\u539f\u83cc\u6838\u9178\u6216\u542b\u7279\u5b9a\u7247\u6bb5\u7684\u8d28\u7c92DNA\uff0c\u5206\u522b\u662f\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83ccSa\u3001\u5ff5\u73e0\u72b6\u94fe\u6746\u83ccSm\u3001\u80ba\u708e\u94fe\u7403\u83ccSp\u3001\u5185\u53c2DNA\u3002<\/p>\n<p>\uff082\uff09\u9633\u6027\u8d28\u63a7\uff08PC2\uff09\u5305\u542b3\u79cd\u75c5\u539f\u83cc\u6838\u9178\u6216\u542b\u7279\u5b9a\u7247\u6bb5\u7684\u8d28\u7c92DNA\uff0c\u5206\u522b\u662f\u03b2-\u6eb6\u8840\u6027\u94fe\u7403\u83cc\u5c5eBHS\u3001\u67e0\u6aac\u9178\u6746\u83ccCr\u3001\u5185\u53c2DNA\u3002<\/p>\n<p>\uff083\uff09\u9633\u6027\u8d28\u63a7\uff08PC3\uff09\u5305\u542b3\u79cd\u75c5\u539f\u83cc\u6838\u9178\u6216\u542b\u7279\u5b9a\u7247\u6bb5\u7684\u8d28\u7c92DNA\uff0c\u5206\u522b\u662f\u725b\u68d2\u72b6\u6746\u83ccCb\u3001\u80ba\u5b62\u5b50\u866bPs\u3001\u5185\u53c2DNA\u3002<\/p>\n<p>\u3010\u64cd\u4f5c\u6b65\u9aa4\u3011<\/p>\n<p>1.\u00a0 \u6837\u672c\u5236\u5907<\/p>\n<p>\u63a8\u8350\u914d\u5957\u4f7f\u7528\u7ffc\u548cSPF-Level Mouse\/Rat Pathgen DNA\/RNA Kit Pro\uff08\u8d27\u53f7\uff1aPRE.M\/R-001\uff09\uff0c\u6b64\u8bd5\u5242\u76d2\u63d0\u53d6\u5feb\u901f\uff0c\u56de\u6536\u7387\u8f83\u9ad8\uff0c<strong>\u5e76\u63d0\u4f9b\u6838\u9178\u4fdd\u62a4\u5242\uff08\u542b\u5185\u90e8\u8d28\u63a7\uff09\uff0c\u53ef\u4ee5\u5bf9\u63d0\u53d6\u8fc7\u7a0b\u8fdb\u884c\u8d28\u63a7<\/strong>\uff0c\u82e5\u4f7f\u7528\u5176\u4ed6\u63d0\u53d6\u8bd5\u5242\u76d2\u9700\u5148\u81ea\u884c\u6d4b\u8bd5\u3002<\/p>\n<p>2.\u00a0 \u4ea7\u54c1\u7ec4\u5206\u7684\u590d\u6eb6<\/p>\n<p>A BOX\uff1aBiowing<sup>\u00ae<\/sup>Optional Bacteria Primer&amp;Probe Mix\u514812,000 rpm\u79bb\u5fc35 min\uff0c\u6309\u4e0b\u8868\u52a0\u5165RNase Free Water\uff0c\u6da1\u65cb\u9707\u83611 min\uff0c\u77ac\u79bb5 s\uff0c\u5ba4\u6e29\u653e\u7f6e5-10 min\u5907\u7528\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"102\">A BOX\uff1a<\/td>\n<td width=\"135\">\u6bcf\u7ba1\u9700\u52a0\u5165RNase Free Water\u91cf<\/td>\n<\/tr>\n<tr>\n<td width=\"102\">Biowing<sup>\u00ae<\/sup>Optional Bacteria Primer&amp;Probe Mix<\/td>\n<td width=\"135\">140 \u03bcL<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\" width=\"236\">\u6ce8\uff1aA\u3001B BOX\u590d\u6eb6\u5206\u522b\u7528\u76d2\u4e2d\u6240\u7ed9\u7684RNase Free Water\uff0c\u8bf7\u52ff\u6df7\u7528\u3001\u590d\u7528\uff0c\u907f\u514d\u6c61\u67d3\uff1b\u4ea7\u54c1\u7ec4\u5206\u590d\u6eb6\u540e\u5efa\u8bae-20\u2103\u4fdd\u5b58\uff0c\u53cd\u590d\u51bb\u878d\u4e0d\u8d85\u8fc7\u4e09\u6b21\uff0c\u53ef\u5206\u88c5\u540e\u50a8\u5b58\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>B BOX\uff1a\u9633\u6027\u8d28\u63a71\uff08PC1\uff09\u3001\u9633\u6027\u8d28\u63a72\uff08PC2\uff09\u3001\u9633\u6027\u8d28\u63a73\uff08PC3\uff09\u514812,000 rpm\u79bb\u5fc35 min\uff0c\u6309\u4e0b\u8868\u52a0\u5165RNase Free Water\uff0c\u6da1\u65cb\u9707\u83611 min\uff0c\u77ac\u79bb5 s\uff0c\u5ba4\u6e29\u653e\u7f6e5-10 min\u5907\u7528\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"102\">B BOX\uff1a<\/td>\n<td width=\"135\">\u6bcf\u7ba1\u9700\u52a0\u5165RNase Free Water\u91cf<\/td>\n<\/tr>\n<tr>\n<td width=\"102\">\u9633\u6027\u8d28\u63a71\uff08PC1\uff09<\/td>\n<td width=\"135\">44 \u03bcL<\/td>\n<\/tr>\n<tr>\n<td width=\"102\">\u9633\u6027\u8d28\u63a72\uff08PC2\uff09<\/td>\n<td width=\"135\">44 \u03bcL<\/td>\n<\/tr>\n<tr>\n<td width=\"102\">\u9633\u6027\u8d28\u63a73\uff08PC3\uff09<\/td>\n<td width=\"135\">44 \u03bcL<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\" width=\"236\">\u6ce8\uff1aA\u3001B BOX\u590d\u6eb6\u5206\u522b\u7528\u76d2\u4e2d\u6240\u7ed9\u7684RNase Free Water\uff0c\u8bf7\u52ff\u6df7\u7528\u3001\u590d\u7528\uff0c\u907f\u514d\u6c61\u67d3\uff1b\u9633\u6027\u8d28\u63a7\u52a1\u5fc5\u4e0eA BOX\u5206\u5f00\u4fdd\u5b58\uff0c\u9632\u6b62\u6c61\u67d3\uff0c\u590d\u6eb6\u540e\u7acb\u5373\u901a\u98ce\u5e76\u55b7\u6d12\u9152\u7cbe\uff0c\u4ee5\u9632\u6c14\u6eb6\u80f6\u6c61\u67d3\uff1b\u4ea7\u54c1\u7ec4\u5206\u590d\u6eb6\u540e\u5efa\u8bae-20\u2103\u4fdd\u5b58\uff0c\u53cd\u590d\u51bb\u878d\u4e0d\u8d85\u8fc7\u4e09\u6b21\uff0c\u53ef\u5206\u88c5\u540e\u50a8\u5b58\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>3.\u00a0 qPCR \u53cd\u5e94\u6db2\u7684\u51c6\u5907<\/p>\n<p>\uff081\uff09\u6839\u636e\u6240\u8981\u68c0\u6d4b\u6837\u54c1\u7684\u6570\u91cf\uff0c\u8ba1\u7b97\u6240\u9700\u53cd\u5e94\u5b54\u6570\uff0c\u6bcf\u4e2a\u6837\u672c\u505a\u5355\u5b54\u3002<\/p>\n<p>\u53cd\u5e94\u5b54\u6570=3\u4e2a\u9633\u6027\u8d28\u63a7+1\u4e2a\u9634\u6027\u8d28\u63a7+\u6837\u672c\u6570<\/p>\n<p>\uff082\uff09\u6839\u636e\u53cd\u5e94\u5b54\u6570\u8ba1\u7b97\u6240\u9700\u7684Biowing<sup>\u00ae<\/sup>Bacteria Probe qPCR Mix\u603b\u91cf\uff08\u9700\u67091\u5b54\u7684\u52a0\u6837\u635f\u5931\u91cf\uff09\uff1a<\/p>\n<p>Biowing<sup>\u00ae<\/sup>Bacteria Probe qPCR Mix=\uff08\u53cd\u5e94\u5b54\u6570+1\uff09\u00d715 \u03bcL<\/p>\n<p>\uff083\uff09\u5404\u8bd5\u5242\u653e\u5ba4\u6e29\u878d\u5316\uff0c\u5e76\u6839\u636e\u4e0b\u8868\u6240\u793a\u51c6\u5907 qPCR Mix\uff1a<\/p>\n<table width=\"240\">\n<tbody>\n<tr>\n<td width=\"59\">\u7ec4\u5206\/\u6837\u54c1\u7ba1<\/td>\n<td width=\"65\">Biowing<sup>\u00ae<\/sup>Bacteria qPCR Reaction Mix (<strong>\u00b5<\/strong>L)<\/td>\n<td width=\"88\">Biowing<sup>\u00ae<\/sup>Optional Bacteria Primer&amp;Probe Mix<\/p>\n<p>(<strong>\u00b5<\/strong>L)<\/td>\n<td width=\"28\">\u603b\u4f53\u79ef<\/p>\n<p>(<strong>\u00b5<\/strong>L)<\/td>\n<\/tr>\n<tr>\n<td width=\"59\">1\u5b54<\/td>\n<td width=\"65\">10<\/td>\n<td width=\"88\">5<\/td>\n<td width=\"28\">15<\/td>\n<\/tr>\n<tr>\n<td width=\"59\">N\u5b54<\/p>\n<p>\uff08\u53cd\u5e94\u5b54\u6570+1\uff09<\/td>\n<td width=\"65\">10N<\/td>\n<td width=\"88\">5N<\/td>\n<td width=\"28\">15N<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>4.\u00a0 qPCR \u53cd\u5e94\u4f53\u7cfb\u53ca\u53cd\u5e94\u6761\u4ef6<\/p>\n<p>\uff081\uff09\u9707\u8361\u6df7\u5300Biowing<sup>\u00ae<\/sup>Bacteria Probe qPCR Mix\uff0c\u4f4e\u901f\u79bb\u5fc3\uff0c\u5c06\u7ba1\u76d6\u6b8b\u7559\u6db2\u4f53\u6536\u96c6\u81f3\u7ba1\u5e95\u3002<\/p>\n<p>\uff082\uff09\u5411\u6bcf\u5b54\u53cd\u5e94\u7ba1\u4e2d\u5206\u88c515 \u03bcL qPCR Mix\u3002<\/p>\n<p>\uff083\uff09\u5411\u5df2\u5206\u88c5\u8fc7qPCR Mix\u7684\u53cd\u5e94\u7ba1\u4e2d\u52a0\u516515 \u03bcL\u77f3\u8721\u6cb9\u3002<\/p>\n<p>\uff084\uff09\u5411\u53cd\u5e94\u7ba1\u4e2d\u52a0\u5165\u6837\u54c1\uff0c3,000 rpm \u77ac\u79bb5 s\uff0c\u52a0\u6837\u793a\u4f8b\u5982\u4e0b\uff1a<\/p>\n<table width=\"249\">\n<tbody>\n<tr>\n<td width=\"44\">\u7ec4\u5206\/\u6837\u54c1\u7ba1<\/td>\n<td width=\"38\">\u5f85\u6d4b\u6837\u54c1\u7ba1<\/p>\n<p>(\u00b5L)<\/td>\n<td width=\"42\">\u9633\u6027\u5bf9\u7167\u7ba11<\/p>\n<p>(\u00b5L)<\/td>\n<td width=\"44\">\u9633\u6027\u5bf9\u7167\u7ba12<\/p>\n<p>(\u00b5L)<\/td>\n<td width=\"42\">\u9633\u6027\u5bf9\u7167\u7ba13<\/p>\n<p>(\u00b5L)<\/td>\n<td width=\"39\">\u9634\u6027\u5bf9\u7167\u7ba1<\/p>\n<p>(\u00b5L)<\/td>\n<\/tr>\n<tr>\n<td width=\"44\">Biowing<sup>\u00ae<\/sup> Bacteria Probe<\/p>\n<p>qPCR Mix<\/td>\n<td width=\"38\">15<\/td>\n<td width=\"42\">15<\/td>\n<td width=\"44\">15<\/td>\n<td width=\"42\">15<\/td>\n<td width=\"39\">15<\/td>\n<\/tr>\n<tr>\n<td width=\"44\">RNase Free Water<\/td>\n<td width=\"38\">0<\/td>\n<td width=\"42\">0<\/td>\n<td width=\"44\">0<\/td>\n<td width=\"42\">0<\/td>\n<td width=\"39\">5<\/td>\n<\/tr>\n<tr>\n<td width=\"44\">Sample<\/td>\n<td width=\"38\">5<\/td>\n<td width=\"42\">0<\/td>\n<td width=\"44\">0<\/td>\n<td width=\"42\">0<\/td>\n<td width=\"39\">0<\/td>\n<\/tr>\n<tr>\n<td width=\"44\">\u9633\u6027\u8d28\u63a71\uff08PC1\uff09<\/td>\n<td width=\"38\">0<\/td>\n<td width=\"42\">5<\/td>\n<td width=\"44\">0<\/td>\n<td width=\"42\">0<\/td>\n<td width=\"39\">0<\/td>\n<\/tr>\n<tr>\n<td width=\"44\">\u9633\u6027\u8d28\u63a72\uff08PC2\uff09<\/td>\n<td width=\"38\">0<\/td>\n<td width=\"42\">0<\/td>\n<td width=\"44\">5<\/td>\n<td width=\"42\">0<\/td>\n<td width=\"39\">0<\/td>\n<\/tr>\n<tr>\n<td width=\"44\">\u9633\u6027\u8d28\u63a73\uff08PC2\uff09<\/td>\n<td width=\"38\">0<\/td>\n<td width=\"42\">0<\/td>\n<td width=\"44\">0<\/td>\n<td width=\"42\">5<\/td>\n<td width=\"39\">0<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>PCR <\/strong><strong>\u4eea\u8bbe\u7f6e\u4ee5\u5b8f\u77f3<\/strong><strong>SLAN-96S<\/strong><strong>\u4e3a\u4f8b\uff0c\u5148\u65b0\u5efa\u5e76\u4fdd\u5b58\u7a0b\u5e8f\u9879\u76ee\uff0c\u9009\u62e9\u5b9a\u6027<\/strong><strong>\/<\/strong><strong>\u7edd\u5bf9\u5b9a\u91cf\u6a21\u5f0f\uff0c\u53cd\u5e94\u4f53\u7cfb<\/strong><strong>20 \u00b5L<\/strong><strong>\uff0c\u9009\u62e9<\/strong><strong>FAM<\/strong><strong>\u3001<\/strong><strong>HEX<\/strong><strong>\u3001<\/strong><strong>ROX<\/strong><strong>\u3001<\/strong><strong>CY5<\/strong><strong>\u901a\u9053\uff0c\u6309\u7167\u4e0b\u8868\u8bbe\u7f6e\u7a0b\u5e8f\u8fdb\u884c\u4fdd\u5b58\uff1a<\/strong><\/p>\n<table width=\"231\">\n<tbody>\n<tr>\n<td colspan=\"2\" width=\"70\">\u6b65\u9aa4<\/td>\n<td width=\"42\">\u5faa\u73af\u6570<\/td>\n<td width=\"59\">\u6e29\u5ea6<\/td>\n<td width=\"59\">\u65f6\u95f4<\/td>\n<\/tr>\n<tr>\n<td width=\"20\">1<\/td>\n<td width=\"50\">\u9006\u8f6c\u5f55<\/td>\n<td width=\"42\">1<\/td>\n<td width=\"59\">55\u2103<\/td>\n<td width=\"59\">15 min<\/td>\n<\/tr>\n<tr>\n<td width=\"20\">2<\/td>\n<td width=\"50\">\u9884\u53d8\u6027<\/td>\n<td width=\"42\">1<\/td>\n<td width=\"59\">95\u2103<\/td>\n<td width=\"59\">30 s<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"2\" width=\"20\">3<\/td>\n<td width=\"50\">\u53d8\u6027<\/td>\n<td rowspan=\"2\" width=\"42\">35\/40<\/td>\n<td width=\"59\">95\u2103<\/td>\n<td width=\"59\">10 s<\/td>\n<\/tr>\n<tr>\n<td width=\"50\">\u9000\u706b\u4e0e\u5ef6\u4f38<\/td>\n<td width=\"59\">60\u2103<\/td>\n<td width=\"59\">30 s<\/td>\n<\/tr>\n<tr>\n<td colspan=\"5\" width=\"231\">60\u2103\u5ef6\u4f38\u65f6\u6536\u96c6\u8367\u5149\u4fe1\u53f7<\/td>\n<\/tr>\n<tr>\n<td colspan=\"5\" width=\"231\">\u901a\u9053\u9009\u62e9\uff1a\u6837\u672c\u68c0\u6d4b\u901a\u9053-FAM\u3001HEX\u3001ROX\uff1b\u5185\u53c2\u68c0\u6d4b\u901a\u9053-CY5\u3002\u5b8f\u77f3SLAN-96S\u8367\u5149\u5b9a\u91cf PCR \u4eea\u4e0d\u9700 ROX \u6821\u51c6\u3002\u82e5\u5b58\u5728\u90e8\u5206\u8367\u5149\u5b9a\u91cfPCR\u4eea\u65e0CY5\u901a\u9053\uff0c\u5373\u4e0d\u5206\u6790\u8be5\u901a\u9053\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u6ce8\uff1a<\/strong>\u6837\u672c\u7c7b\u578b\u4e3a\u7caa\u4fbf\/\u76f2\u80a0\u5185\u5bb9\u7269\u65f6\u53d8\u6027\u3001\u9000\u706b\/\u5ef6\u4f38\u8fdb\u884c35\u4e2a\u5faa\u73af\u6269\u589e\uff0c\u5176\u4ed6\u6837\u672c\u7c7b\u578b\u53d8\u6027\u3001\u9000\u706b\/\u5ef6\u4f38\u8fdb\u884c40\u4e2a\u5faa\u73af\u6269\u589e\u3002<\/p>\n<p>\u3010\u9608\u503c\u8bbe\u7f6e\u3011<\/p>\n<p>\u4ee5\u5b8f\u77f3SLAN-96S\u4e3a\u4f8b\uff0c\u5e38\u7528\u9009\u9879\u4e2d\u6269\u589e\u66f2\u7ebf\u7b97\u6cd5\u9009\u62e9\u76f8\u5bf9\u8367\u5149\u503c\u6cd5-log\uff0c\u57fa\u7ebf\u8bbe\u7f6e3-15\u4e2a\u5faa\u73af\u3002<\/p>\n<p>\uff081\uff09\u6309\u7167\u673a\u5668\u7684\u81ea\u52a8\u9608\u503c\u6216\u8005\u624b\u52a8\u8c03\u6574\u9608\u503c\u7ebf\u5c06\u9633\u6027\u8d28\u63a7\uff08PC\uff09\u5404\u901a\u9053\u7684Ct\u503c\u5b9a\u4e3a\u226432\uff0c\u6839\u636e\u8be5\u9608\u503c\u7ebf\u8fdb\u884c\u68c0\u6d4b\u6837\u672c\u7ed3\u679c\u7684\u5224\u5b9a\u3002<\/p>\n<p>\u3010\u7ed3\u679c\u5224\u8bfb\u3011<\/p>\n<p>\u4ee5\u5b8f\u77f3SLAN-96S\u4e3a\u4f8b\uff0c\u6839\u636e\u6837\u672c\uff08FAM\/HEX\/ROX\uff09Ct\u503c\u5224\u5b9a\u662f\u5426\u53d1\u751f\u75c5\u539f\u83cc\u611f\u67d3\uff0c\u5177\u4f53\u6807\u51c6\u5982\u4e0b\uff1a<\/p>\n<table width=\"232\">\n<tbody>\n<tr>\n<td width=\"56\">PCR \u6a21\u677f<\/td>\n<td width=\"119\">\u6837\u672c\uff08FAM\/HEX\/ROX\uff09<\/td>\n<td width=\"57\">\u5224\u5b9a\u8bf4\u660e<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"2\" width=\"56\">\u9633\u6027\u5bf9\u7167<\/p>\n<p>\uff081\u30012\u30013\uff09<\/td>\n<td width=\"119\">\u226432<\/td>\n<td width=\"57\">\u9633\u6027\u6210\u7acb<\/td>\n<\/tr>\n<tr>\n<td width=\"119\">\uff1e32<\/td>\n<td width=\"57\">\u9633\u6027\u4e0d\u6210\u7acb<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"2\" width=\"56\">\u9634\u6027\u5bf9\u7167<\/td>\n<td width=\"119\">\uff1e35\uff08\u7caa\u4fbf\/\u76f2\u80a0\u5185\u5bb9\u7269\uff09<\/td>\n<td rowspan=\"2\" width=\"57\">\u9634\u6027\u6210\u7acb<\/td>\n<\/tr>\n<tr>\n<td width=\"119\">\uff1e40\uff08\u7ec4\u7ec7\u3001\u8840\u6db2\u3001\u57ab\u6599\/\u7c89\u5c18\uff09<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"2\" width=\"56\">\u5f85\u6d4b\u6837\u54c1<\/td>\n<td width=\"119\">\u226435\uff08\u7caa\u4fbf\/\u76f2\u80a0\u5185\u5bb9\u7269\uff09<\/td>\n<td rowspan=\"2\" width=\"57\">\u9633\u6027\u6210\u7acb<\/td>\n<\/tr>\n<tr>\n<td width=\"119\">\u226440\uff08\u7ec4\u7ec7\u3001\u8840\u6db2\u3001\u57ab\u6599\/\u7c89\u5c18\uff09<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u6ce8\uff1a<\/strong><\/p>\n<ol>\n<li>\u6837\u672c\u9633\u6027\u5224\u65ad\u6807\u51c6\u4e3a\uff1a<\/li>\n<\/ol>\n<p>\uff081\uff09\u7caa\u4fbf\/\u76f2\u80a0\u5185\u5bb9\u7269\u6837\u672cCt\u503c\u226435\u65f6\uff0c\u5224\u5b9a\u4e3a\u9633\u6027\u3002\u5176\u4e2dFAM\u901a\u9053Ct\u503c\u226435\u65f6\uff0c\u6837\u672c\u53ef\u80fd\u611f\u67d3\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83ccSa\u3001\u03b2-\u6eb6\u8840\u6027\u94fe\u7403\u83cc\u5c5eBHS\u3001\u725b\u68d2\u72b6\u6746\u83ccCb\u4e2d\u7684\u4e00\u79cd\u6216\u51e0\u79cd\uff1bHEX\u901a\u9053Ct\u503c\u226435\u65f6\uff0c\u6837\u672c\u53ef\u80fd\u611f\u67d3\u5ff5\u73e0\u72b6\u94fe\u6746\u83ccSm\u3001\u67e0\u6aac\u9178\u6746\u83ccCr\u3001\u80ba\u5b62\u5b50\u866bPs\u4e2d\u7684\u4e00\u79cd\u6216\u51e0\u79cd\uff1bROX\u901a\u9053Ct\u503c\u226435\u65f6\uff0c\u6837\u672c\u611f\u67d3\u80ba\u708e\u94fe\u7403\u83ccSp\u3002<\/p>\n<p>\uff082\uff09\u7ec4\u7ec7\u3001\u8840\u6db2\u3001\u57ab\u6599\/\u7c89\u5c18\u6837\u672cCt\u503c\u226440\u65f6\uff0c\u5224\u5b9a\u4e3a\u9633\u6027\u3002\u5176\u4e2dFAM\u901a\u9053Ct\u503c\u226440\uff0c\u6837\u672c\u53ef\u80fd\u611f\u67d3\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83ccSa\u3001\u03b2-\u6eb6\u8840\u6027\u94fe\u7403\u83cc\u5c5eBHS\u3001\u725b\u68d2\u72b6\u6746\u83ccCb\u4e2d\u7684\u4e00\u79cd\u6216\u51e0\u79cd\uff1bHEX\u901a\u9053Ct\u503c\u226440\uff0c\u6837\u672c\u53ef\u80fd\u611f\u67d3\u5ff5\u73e0\u72b6\u94fe\u6746\u83ccSm\u3001\u67e0\u6aac\u9178\u6746\u83ccCr\u3001\u80ba\u5b62\u5b50\u866bPs\u4e2d\u7684\u4e00\u79cd\u6216\u51e0\u79cd\uff1bROX\u901a\u9053Ct\u503c\u226440\uff0c\u6837\u672c\u611f\u67d3\u80ba\u708e\u94fe\u7403\u83ccSp\u3002<\/p>\n<p>\uff083\uff09\u4e0d\u540c\u673a\u5668\u7684\u68c0\u6d4b\u7075\u654f\u5ea6\u4e0d\u540c\uff0c\u4e0d\u540c\u6837\u672c\u7c7b\u578b\u6027\u8d28\u4e0d\u540c\uff0c\u5efa\u8bae\u4f7f\u7528\u8be5\u7cfb\u5217\u8bd5\u5242\u76d2\u68c0\u6d4b\u6837\u672c\u524d\uff0c\u5229\u7528SPF\u6837\u672c\u8fdb\u884c\u9002\u5e94\u6027\u9a8c\u8bc1\u540e\u5236\u5b9a\u9633\u6027\u5224\u65ad\u6807\u51c6\u3002<\/p>\n<ol start=\"2\">\n<li>\u82e5\u5404\u901a\u9053\u6709\u9633\u6027\u51fa\u73b0\uff0c\u5efa\u8bae\u8fdb\u4e00\u6b65\u4f7f\u7528\u9274\u5b9a\u8bd5\u5242\u76d2\u8fdb\u884c\u5355\u4e00\u75c5\u539f\u4f53\u611f\u67d3\u786e\u8ba4\uff1a<\/li>\n<\/ol>\n<p>\uff081\uff09FAM\u901a\u9053\u9633\u6027\uff1a\u4f7f\u7528SPF.M\/R-OBF-004\u8bd5\u5242\u76d2\uff0c\u5bf9\u4e09\u79cd\u53ef\u80fd\u611f\u67d3\u7684\u75c5\u539f\u83cc\uff08\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83ccSa\u3001\u03b2-\u6eb6\u8840\u6027\u94fe\u7403\u83cc\u5c5eBHS\u3001\u725b\u68d2\u72b6\u6746\u83ccCb\uff09\u8fdb\u884c\u786e\u8ba4\u3002<\/p>\n<p>\uff082\uff09HEX\u901a\u9053\u9633\u6027\uff1a\u4f7f\u7528SPF.M\/R-OBH-005\u8bd5\u5242\u76d2\uff0c\u5bf9\u4e09\u79cd\u53ef\u80fd\u611f\u67d3\u7684\u75c5\u539f\u83cc\uff08\u5ff5\u73e0\u72b6\u94fe\u6746\u83ccSm\u3001\u67e0\u6aac\u9178\u6746\u83ccCr\u3001\u80ba\u5b62\u5b50\u866bPs\uff09\u8fdb\u884c\u786e\u8ba4\u3002<\/p>\n<ol start=\"3\">\n<li>\u68c0\u67e5\u5185\u53c2\u901a\u9053\u6269\u589e\u662f\u5426\u6b63\u5e38\uff0c\u5185\u53c2\u6269\u589e\u4e0d\u6b63\u5e38\uff0c\u8868\u660e\u6838\u9178\u62bd\u63d0\u8fc7\u7a0b\u6709\u8bef\u6216\u8005PCR\u53cd\u5e94\u672a\u6b63\u5e38\u8fdb\u884c\uff0c\u9700\u91cd\u590d\u68c0\u6d4b\u3002<\/li>\n<\/ol>\n<p>\u3010\u6ce8\u610f\u4e8b\u9879\u3011<\/p>\n<p>\u7531\u4e8ePCR\u53cd\u5e94\u975e\u5e38\u7075\u654f\uff0c\u5b9e\u9a8c\u64cd\u4f5c\u4e2d\u5e94\u6ce8\u610f\u4ee5\u4e0b\u4e8b\u9879\uff0c\u4ee5\u514d\u53d1\u751f\u6838\u9178\u6c61\u67d3\u3002<\/p>\n<ol>\n<li><strong>\u5efa\u8bae\u5206\u533a\u64cd\u4f5c<\/strong><\/li>\n<\/ol>\n<p>\uff081\uff09A\u533a\uff1a\uff08\u6d01\u51c0\u533a\uff09\u914d\u5236Mix\u548cqPCR\u9634\u6027\u52a0\u6837\uff0c\u5efa\u8bae\u5728\u8d85\u51c0\u5de5\u4f5c\u53f0\u5b8c\u6210\uff0c\u5e76\u914d\u5957\u5e72\u51c0\u5668\u5177\u53ca\u65e0\u83cc\u65e0\u9176\u8017\u6750\u4e13\u7528\u4e8e\u4f53\u7cfb\u914d\u5236\u3002<\/p>\n<p>\uff082\uff09B\u533a\uff1a\uff08\u6838\u9178\u63d0\u53d6\u53ca\u52a0\u6837\u533a\uff09\u6837\u672cDNA\/RNA\u63d0\u53d6\uff0c\u5efa\u8bae\u5728\u8d85\u51c0\u5de5\u4f5c\u53f0\u5b8c\u6210\uff0c\u82e5\u65e0\u8d85\u51c0\u5de5\u4f5c\u53f0\u8bf7\u901a\u98ce\u72b6\u6001\u4e0b\u63d0\u53d6\u5e76\u7acb\u5373\u6d88\u6740\uff0c\u9700\u914d\u5957\u4e13\u7528\u4e8e\u6838\u9178\u63d0\u53d6\u7684\u5668\u5177\uff0c\u5e76\u5728\u6bcf\u6b21\u63d0\u53d6\u540e\u5bf9\u73af\u5883\u548c\u5668\u5177\u8fdb\u884c\u6838\u9178\u6d88\u6740\u5904\u7406\uff1b\u52a0\u6837\u533a\uff0c\u6309\u7167\u5148\u52a0\u5f85\u6d4b\u6837\u672c\uff0c\u540e\u52a0\u9633\u6027\u5bf9\u7167\u7684\u987a\u5e8f\u52a0\u6837\uff0c\u5728qPCR\u53cd\u5e94\u677f\u4e0a\u9633\u6027\u5bf9\u7167\u7684\u6392\u5e03\u5e94\u8fdc\u79bb\u5f85\u6d4b\u6837\u672c\u5b54\u548c\u9634\u6027\u5bf9\u7167\uff1b\u540c\u6837\u9700\u914d\u5957\u4e13\u7528\u4e8e\u52a0\u6837\u7684\u4eea\u5668\uff0c\u5e76\u5728\u6bcf\u6b21\u52a0\u5b8c\u6837\u540e\u8fdb\u884c\u6838\u9178\u6d88\u6740\u5904\u7406\u3002<\/p>\n<p>\uff083\uff09C\u533a\uff1a\uff08\u6838\u9178\u6269\u589e\u533a\uff09\u6b64\u533a\u57df\u653e\u7f6e\u8367\u5149\u5b9a\u91cfPCR\u4eea\u53ca\u76f8\u5173\u7535\u8111\u8fdb\u884c\u6570\u636e\u5904\u7406\uff0c\u540c\u6837\u9700\u5b9a\u671f\u8fdb\u884c\u6838\u9178\u6d88\u6740\u3002<\/p>\n<ol 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