{"id":434,"date":"2025-03-27T22:51:56","date_gmt":"2025-03-27T14:51:56","guid":{"rendered":"https:\/\/www.biowing.com.cn\/?post_type=product&p=434"},"modified":"2025-04-02T22:36:57","modified_gmt":"2025-04-02T14:36:57","slug":"xbjysjhpdxmxxsxbjrztjcsjh","status":"publish","type":"product","link":"https:\/\/www.biowing.com.cn\/index.php\/product\/xbjysjhpdxmxxsxbjrztjcsjh\/","title":{"rendered":"PDX\u6a21\u578b\u5c0f\u9f20\u7ec6\u80de\u6d78\u6da6\u72b6\u6001\u68c0\u6d4b\u8bd5\u5242\u76d2"},"content":{"rendered":"

\u3010\u4ea7\u54c1\u89c4\u683c\u3011<\/span><\/span><\/strong><\/h1>\n

PDX<\/span><\/span>50<\/span>\u00a0\u00a0<\/span>50<\/span>T<\/span><\/span><\/p>\n

\u3010\u4ea7\u54c1\u8bf4\u660e\u3011<\/span><\/span><\/strong><\/h1>\n

\u4eba\u6e90\u80bf\u7624\u5f02\u79cd\u79fb\u690d\u6a21\u578b\uff08<\/span>Patient-Derived tumor Xenograft , PDX<\/span>\uff09\u662f\u5c06\u75c5\u4eba\u7684\u80bf\u7624\u7ec4\u7ec7\u76f4\u63a5\u79fb\u690d\u5230\u514d\u75ab\u7f3a\u9677\u9f20\u800c\u5efa\u7acb\u7684\u4eba\u6e90\u5f02\u79cd\u79fb\u690d\u6a21\u578b\uff0c\u662f\u764c\u75c7\u673a\u5236\u7814\u7a76\u548c\u836f\u7269\u6d4b\u8bd5\u7684\u6709\u7528\u5de5\u5177\uff0c\u4f46\u968f\u7740\u4f20\u4ee3\u6b21\u6570\u589e\u52a0\uff0c\u80bf\u7624\u7ec4\u7ec7\u4fdd\u771f\u5ea6\u4e0b\u964d\u3002<\/span><\/span><\/p>\n

\u672c\u4ea7\u54c1\u8bd5\u5242\u76d2\u91c7\u7528<\/span><\/span>\u53cc\u8272\u8367\u5149<\/span><\/span>\u63a2\u9488<\/span>\u00a0<\/span>qPCR<\/span>\uff08<\/span><\/span>FAM<\/span><\/span>+<\/span>HEX<\/span><\/span>\uff09<\/span><\/span>\u68c0\u6d4b<\/span><\/span>PDX<\/span>\u6a21\u578b\u603b<\/span>DNA<\/span>\u4e2d\u5c0f\u9f20<\/span>DNA<\/span>\u7684\u6bd4\u4f8b\uff0c\u5e76\u4ee5\u6b64\u63a8\u5bfc\u5c0f\u9f20\u7ec6\u80de\u6d78\u6da6\u6bd4\u4f8b<\/span><\/span>\uff0c\u68c0\u6d4b\u5bf9\u8c61\u4e3a<\/span><\/span>\u7ec4\u7ec7<\/span>DNA\u3002\u8be5\u8bd5\u5242\u76d2\u5177\u6709\u4ee5\u4e0b\u7279\u70b9<\/span><\/span>\uff1a<\/span><\/p>\n

\u7a33\u5b9a<\/span><\/span><\/span>\uff1a\u5168\u7a0b\u95ed\u7ba1\u64cd\u4f5c\uff0c\u65e0\u4ea4\u53c9\u6c61\u67d3\u3002<\/span><\/span>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/p>\n

1.\u00a0<\/span>\u53ef\u9760<\/span><\/span><\/span>\uff1a<\/span><\/span>\u6837\u672c\u4e0e\u591a\u4e2a<\/span>\u5185\u6807\u540c\u677f<\/span>\u68c0\u6d4b\uff0c\u51cf\u5c11\u5b54\u95f4\u5e72\u6270\u3002<\/span><\/span><\/p>\n

2.\u00a0<\/span>\u65b9\u4fbf<\/span><\/span><\/span>\uff1a\u64cd\u4f5c\u7b80\u5355\uff0c\u65e0\u9700\u7535\u6cf3\u6b65\u9aa4\u3002<\/span><\/span><\/p>\n

3.\u00a0<\/span>\u5b9a\u91cf<\/span><\/span><\/span>\uff1a<\/span> <\/span>\u25b3<\/span>CT<\/span>\u00a0<\/span>\u503c\uff0c\u53ef\u5b9a\u91cf\u68c0\u6d4b\u3002<\/span><\/span><\/p>\n

\u00a0<\/span><\/p>\n

\u3010\u8fd0\u8f93\u53ca\u4fdd\u5b58\u6761\u4ef6\u3011<\/span><\/span><\/strong><\/h1>\n

\u4f4e\u6e29\u51b7\u51bb\u8fd0\u8f93\uff0c<\/span> <\/span>-20<\/span>\u2103<\/span>\u4fdd\u5b58\uff0c\u6709\u6548\u671f<\/span> <\/span>1 <\/span>\u5e74\uff0c\u51bb\u878d\u4ee5\u540e\uff0c<\/span>4<\/span>\u2103<\/span>\u4fdd\u5b58\uff0c\u6709\u6548\u671f<\/span>4\u5468\u3002<\/span><\/span><\/p>\n

\u3010\u4ea7\u54c1\u7ec4\u5206\u3011<\/span><\/span><\/strong><\/h1>\n

<\/span><\/span><\/strong><\/p>\n

\u00a0<\/span><\/strong><\/p>\n

\u6ce8\uff1a<\/span><\/strong>\uff08<\/span>1<\/span>\uff09<\/span>Biowing\u00aePDX qPCR SuperMix<\/span><\/span>\u542b\u5f15\u7269\u4e0e\u5176\u4ed6<\/span>PCR\u6210\u5206\uff1b<\/span><\/span>\uff08<\/span>2<\/span>\uff09<\/span><\/span>Standard<\/span>\u5305\u542b\u5c0f\u9f20<\/span>DNA<\/span>\u548c\u4eba\u57fa\u56e0\u7ec4<\/span>DNA<\/span>\uff0c<\/span><\/span>Standard<\/span>1<\/span>\u3001<\/span>2<\/span>\u548c<\/span>3<\/span>\u5206\u522b\u5bf9\u5e94\u5c0f\u9f20<\/span>DNA<\/span>\u542b\u91cf\u4e3a<\/span><\/span>25<\/span><\/span>%<\/span>\u3001<\/span><\/span>50<\/span><\/span>%<\/span>\u548c<\/span><\/span>75<\/span><\/span>%<\/span>\uff1b<\/span><\/span>(<\/span>3<\/span><\/span>)\u672c\u8bd5\u5242\u76d2\u63d0\u4f9b\u8bd5\u5242\uff0c\u4f7f\u7528\u524d\u8bf7\u4e0a\u4e0b\u8f7b\u67d4\u98a0\u5012\u5341\u6b21\u6df7\u5300\uff0c\u907f\u514d\u8d77<\/span><\/span>\u6ce1<\/span><\/span>\uff0c<\/span>\u4f4e\u901f\u79bb\u5fc3\u3001\u5c0f\u5fc3\u5f00\u542f<\/span><\/span>\u540e\u4f7f\u7528<\/span>\u3002<\/span><\/span><\/p>\n

\u3010\u9608\u503c\u8bbe\u7f6e\u3011<\/span><\/strong>\u4ee5<\/span>50<\/span><\/span>%<\/span>\u6807\u51c6\u54c1<\/span>Standard<\/span><\/span>2<\/span><\/span>\u6269\u589e\u66f2\u7ebf\uff0c\u8bbe\u7f6e\u9608\u503c\u7ebf\uff0c\u6ee1\u8db3\u4e0b\u5217<\/span>CT<\/span>\u503c\u8303\u56f4<\/span><\/span><\/p>\n

\uff081\uff09<\/span>\u4eba<\/span>DNA<\/span>\u6269\u589e\u66f2\u7ebf<\/span>CT<\/span>\u503c<\/span><\/span>\u5b9a\u4e3a<\/span>25<\/span><\/span>\u00b1<\/span>1<\/span>\uff1b<\/span><\/span><\/p>\n

\uff08<\/span>2<\/span>\uff09\u5c0f\u9f20<\/span>DNA<\/span>\u6269\u589e\u66f2\u7ebf<\/span>CT<\/span>\u503c\u5b9a\u4e3a<\/span><\/span>24<\/span><\/span>\u00b1<\/span>1<\/span>\u3002<\/span><\/span><\/p>\n

\u3010\u64cd\u4f5c\u6b65\u9aa4\u3011<\/span><\/span><\/strong><\/h1>\n

1.\u00a0<\/span>\u6837<\/span><\/span><\/strong>\u54c1<\/span><\/span><\/strong>\u51c6\u5907<\/span><\/strong><\/h2>\n

\u4f7f\u7528\u5546\u4e1a\u5316<\/span>DNA\u62bd\u63d0\u8bd5\u5242\u76d2<\/span><\/span>\u63d0\u53d6<\/span>\u7ec4\u7ec7<\/span>DNA\uff0c<\/span><\/span>D<\/span><\/span>NA\u7eaf\u5ea6<\/span>A260\/A280\u22481.8<\/span>\uff0c<\/span><\/span>\u5efa\u8bae\u6d53\u5ea6\u4e3a<\/span>20-50ng\/<\/span><\/span>\u00b5L<\/span>\uff0c<\/span>\u4e0a\u6837<\/span><\/span>2<\/span><\/span>\u00b5L<\/span>\u3002<\/span><\/p>\n

\u6ce8<\/span><\/strong>\uff1a<\/span><\/span><\/strong>\u6837\u54c1<\/span><\/span>\u6d53\u5ea6\u8fc7\u9ad8\u6216<\/span>\u8fc7\u4f4e<\/span><\/span>\u90fd<\/span>\u4f1a\u5f71\u54cd\u6269\u589e\u6548\u7387\uff0c\u5bf9\u5b9e\u9a8c\u9020\u6210\u5e72\u6270\uff0c\u6d4b\u91cf\u7ed3\u679c\u8bef\u5dee\u8f83\u5927\u3002<\/span><\/span><\/p>\n

2.\u00a0<\/span>qPCR<\/span><\/strong>\u53cd\u5e94\u4f53\u7cfb\u53ca\u6761\u4ef6<\/span><\/span><\/strong><\/h2>\n

\u53cd\u5e94\u4f53\u7cfb\u4ee5<\/span><\/span><\/span><\/strong>20<\/span><\/span><\/span><\/strong>\u00b5<\/span><\/span><\/strong>L<\/span><\/span><\/strong>\u4e3a\u4f8b<\/span><\/span><\/span><\/strong><\/h3>\n

<\/p>\n

PCR<\/span><\/span><\/strong>\u4eea\u8bbe\u7f6e\u4ee5<\/span><\/span><\/span><\/strong>SLAN-96S<\/span><\/span><\/span><\/strong>\u4e3a\u4f8b<\/span><\/span><\/span><\/strong>\u3002<\/span><\/span><\/span><\/strong><\/p>\n

<\/span><\/span><\/strong><\/p>\n

\u3010<\/span><\/span><\/strong>\u6807\u51c6\u66f2\u7ebf<\/span><\/strong>\u3011<\/span><\/span><\/strong>\u4e3e\u4f8b<\/span><\/p>\n

\u00a0<\/span><\/p>\n

\u6ce8\uff1a\u5efa\u8bae<\/span><\/strong>\u6bcf\u6279\u6b21\u68c0\u6d4b\uff0c\u5747\u7ed8\u5236\u6807\u51c6\u66f2\u7ebf\uff0c\u4e09\u4e2a\u9633\u6027\u6807\u51c6\u54c1\uff0c\u5206\u522b<\/span>3<\/span>\u6b21\u6280\u672f\u91cd\u590d\uff0c\u7ed8\u5236\u6563\u70b9\u56fe\u5e76\u6dfb\u52a0\u7ebf\u6027\u62df\u5408\u8d8b\u52bf\u7ebf\uff0c\u83b7\u5f97\u56de\u5f52\u65b9\u7a0b\uff0c\u7528\u4e8e\u5c0f\u9f20\u7ec6\u80de\u6d78\u6da6\u6bd4\u4f8b\u7684\u8ba1\u7b97\u3002<\/span><\/span><\/p>\n

\u3010<\/span><\/span><\/strong>\u7ed3\u679c\u5224\u8bfb<\/span><\/strong>\u3011<\/span><\/span><\/strong>\u00a0<\/span><\/strong>\u4ee5<\/span>\u6807\u51c6\u66f2\u7ebf\u7684\u7ebf\u6027\u56de\u5f52\u65b9\u7a0b\u4e3a\uff1a<\/span>Y=-0.4061*X+0.4089<\/span>\u4e3a\u4f8b<\/span><\/span><\/p>\n

PDX<\/span>\u6a21\u578b\u4e2d\u7ec6\u80de\u6bd4\u4f8b<\/span><\/span>\u8ba1\u7b97\u65b9\u6cd5\uff08\u9f20<\/span><\/span>:<\/span><\/span>\u4eba\uff09\uff1a\u6839\u636e\u6807\u51c6\u66f2\u7ebf\u8ba1\u7b97<\/span><\/span>PDX<\/span><\/span>\u6837\u672c\u4e2d\u7684\u9f20<\/span><\/span>\u3001<\/span>\u4eba\u7ec6\u80de\u6bd4\u4f8b<\/span><\/span>\u3002<\/span><\/p>\n

\u6ce8\uff1a<\/span><\/strong>\u6807\u51c6\u66f2\u7ebf\u7684\u7ebf\u6027\u56de\u5f52\u65b9\u7a0b\u4e3a\uff1a<\/span>Y=-0.4061*X+0.4089<\/span><\/span><\/p>\n

\u76f8\u5173\u7cfb\u6570<\/span>R<\/span><\/span>2<\/span><\/span><\/sup>\uff1a<\/span>0.9998<\/span><\/span><\/p>\n

X<\/span>\uff1a\u25b3<\/span>Ct<\/span>\u503c\uff08<\/span>CT<\/span><\/span>\u5c0f\u9f20<\/span><\/span><\/sub>-CT<\/span><\/span>\u4eba<\/span><\/span><\/sub>\uff09<\/span><\/p>\n

Y<\/span>\uff1a<\/span>PDX<\/span>\u6a21\u578b\u4e2d\u4eba\u9f20\u6df7\u6837\u6bd4\u4f8b\uff08\u6837\u672c\u6bd4\u4f8b\u4e3a\u5c0f\u9f20\u7ec6\u80de\uff1a\u4eba\u7ec6\u80de\uff09\u7684<\/span>log10<\/span>\u503c\uff0c\u5373<\/span>Y=log<\/span><\/span>10<\/span><\/span><\/sub>(<\/span>\u5c0f\u9f20<\/span>:<\/span>\u4eba\u6bd4\u4f8b<\/span>)<\/span>\u3002<\/span><\/span><\/p>\n

\u5c0f\u9f20<\/span>:<\/span>\u4eba\u6bd4\u4f8b<\/span>=power(10,Y)<\/span>\uff0c<\/span><\/span><\/p>\n

\u5c0f\u9f20<\/span>DNA<\/span><\/span>\u5360\u603b<\/span> DNA \u767e\u5206\u6bd4<\/span>=<\/span><\/span>power(10,Y)\/(1+ power(10,Y))<\/span><\/span><\/p>\n

\u4eba<\/span>DNA<\/span>\u5360\u603b<\/span>DNA<\/span>\u767e\u5206\u6bd4<\/span>=1-<\/span>\u5c0f\u9f20<\/span>DNA<\/span>\u5360\u603b<\/span>DNA<\/span>\u767e\u5206\u6bd4<\/span><\/span><\/p>\n

<\/h1>\n","protected":false},"excerpt":{"rendered":"

\u7ffc\u548c\u751f\u7269\u81ea\u4e3b\u7814\u53d1PDX\u6a21\u578b\u5c0f\u9f20\u7ec6\u80de\u6d78\u6da6\u72b6\u6001\u68c0\u6d4b\u8bd5\u5242\u76d2\uff0c\u91c7\u7528\u5b9a\u91cfPCR\u6cd5\uff0c\u53ef\u68c0\u6d4bPDX\u6a21\u578b\u4e2d\u4eba\u3001\u9f20\u7ec6\u80de\u7684\u6bd4\u4f8b\u3002\u5b9e\u9a8c\u7528\u65f6\u77ed\uff0c\u64cd\u4f5c\u7b80\u5355\uff0c\u7ed3\u679c\u51c6\u786e\uff0c\u9002\u7528\u4e8e\u6279\u91cf\u6837\u672c\u68c0\u6d4b\u3002\u68c0\u6d4b\u5bf9\u8c61\u4e3a\u7ec4\u7ec7\u6216\u7ec6\u80deDNA\u3002<\/p>\n","protected":false},"featured_media":435,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[19,24],"product_tag":[],"class_list":{"0":"post-434","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-cpzx","7":"product_cat-xbjysjh","9":"first","10":"instock","11":"shipping-taxable","12":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/product\/434","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/comments?post=434"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/media\/435"}],"wp:attachment":[{"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/media?parent=434"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/product_brand?post=434"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/product_cat?post=434"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/product_tag?post=434"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}