{"id":602,"date":"2025-04-24T05:23:38","date_gmt":"2025-04-23T21:23:38","guid":{"rendered":"https:\/\/www.biowing.com.cn\/?post_type=product&p=602"},"modified":"2025-07-07T17:50:32","modified_gmt":"2025-07-07T09:50:32","slug":"xbjyfwxbdlmhxjcfw","status":"publish","type":"product","link":"https:\/\/www.biowing.com.cn\/index.php\/product\/xbjyfwxbdlmhxjcfw\/","title":{"rendered":"\u7ec6\u80de\u7aef\u7c92\u9176\u6d3b\u6027\u68c0\u6d4b\u670d\u52a1"},"content":{"rendered":"

\u3010\u4ea7\u54c1\u89c4\u683c\u3011<\/span><\/p>\n

BPT<\/span>5<\/span>0 \u00a050<\/span>T<\/span>\u00a0\u00a0<\/span>(<\/span>50<\/span>\u4eba\u4efd<\/span> \uff0c\u5176\u4e2d<\/span><\/span>5<\/span>\u4e2a\u7ec4\u5206\u6309<\/span>5<\/span>\u4e2a<\/span>10<\/span>\u4eba\u4efd\u5355\u72ec\u5305\u88c5<\/span>\uff0c<\/span><\/span>Biowing<\/span>\u00ae <\/span>Specific<\/span>\u00a0<\/span>Reverse<\/span>\u00a0<\/span>Transcription Primer<\/span>\u6309<\/span>20<\/span>\u00b5<\/span>L<\/span>*2<\/span>\u5355\u72ec\u5305\u88c5\u3002<\/span>)<\/span><\/p>\n

\u3010\u4ea7\u54c1\u8bf4\u660e\u3011<\/span><\/p>\n

\u7aef<\/span><\/span>\u7c92\u9176<\/span> (<\/span>Telomerase<\/span>\u00a0<\/span>) \u662f\u4e00\u79cd\u9176\u4fc3\u6838\u7cd6\u86cb\u767d\u590d\u5408\u7269\u3002\u5176\u50ac\u5316\u4e9a\u57fa (<\/span>TERT<\/span>) \u5177\u6709\u9006\u8f6c\u5f55\u9176\u7684\u7279<\/span><\/p>\n

\u6027\uff0c<\/span>TERT<\/span>\u88ab\u8ba4\u4e3a\u662f\u7aef\u7c92\u9176\u6d3b\u6027\u8868\u8fbe\u7684\u5173\u952e\u7ec4\u5206\uff0c\u5176\u7f16\u7801\u7684<\/span>mRNA<\/span>\u6c34\u5e73\u4e0e\u7aef\u7c92\u9176\u6d3b\u6027\u4e00\u81f4\uff0c\u4e0e\u7aef\u7c92\u9176<\/span>\u7684<\/span>\u00a0<\/span>\u6d3b<\/span>\u5316<\/span>\u7a0b\u5ea6\u5bc6\u5207\u76f8\u5173\u3002\u56e0\u6b64\uff0c\u5bf9\u7aef\u7c92\u9176\u50ac\u5316\u4e9a\u57fa\u7684\u68c0\u6d4b\u53ef\u4ee5\u95f4\u63a5\u53cd\u6620\u6837\u672c\u4e2d\u7aef\u7c92\u9176\u6d3b\u6027\u3002<\/span><\/p>\n

\u672c<\/span>\u4ea7<\/span>\u54c1\u8bd5\u5242\u76d2\u91c7\u7528\u53cc\u8272\u8367\u5149<\/span>qPCR<\/span>\u00a0\u00a0<\/span>(<\/span>TaqMan<\/span>\u63a2\u9488\u6cd5<\/span>) \u68c0\u6d4b\u7aef\u7c92\u9176\u50ac\u5316\u4e9a\u57fa<\/span>TERT<\/span>\u57fa\u56e0\u548c\u5185\u53c2\u57fa\u56e0<\/span>***DH<\/span>\u00a0<\/span>\u3002\u901a\u8fc7\u63d0\u53d6\u7ec6\u80de<\/span>RNA<\/span>\u00a0<\/span>\uff0c\u5229\u7528\u7279\u5f02\u6027\u9006\u8f6c\u5f55\u5f15\u7269\u8fdb\u884c\u9006\u8f6c\u5f55\u53cd\u5e94\uff0c<\/span> \u4ee5<\/span><\/span>cDNA<\/span>\u4e3a\u6a21\u677f\u5728\u5355\u7ba1\u4e2d\u5229\u7528<\/span>\u591a<\/span>\u91cd\u8367\u5149\u5b9a\u91cf<\/span>PCR<\/span>\u8fdb\u884c\u6269\u589e\u53cd\u5e94<\/span> (\u53cc\u91cd\u63a2\u9488\uff1a<\/span>FAM<\/span>\u00a0<\/span>\u3001<\/span>Cy<\/span>5<\/span>) \u3002\u9009\u7528<\/span>293<\/span>T<\/span>\u7ec6\u80de\u4e3a\u9633\u6027\u5bf9\u7167\u4ee5\u53ca<\/span>MRC<\/span>-5<\/span>\u7ec6<\/span>\u80de<\/span>\u4e3a<\/span>\u9634\u6027\u5bf9\u7167\uff0c<\/span>\u5224\u65ad\u6837\u672c\u4e2d\u662f\u5426\u6709<\/span>TERT<\/span>\u57fa\u56e0\u8868\u8fbe<\/span>\u3002\u8be5\u8bd5\u5242\u76d2\u5177\u6709\u4ee5\u4e0b\u7279\u70b9\uff1a<\/span><\/p>\n

1<\/span>. <\/span>\u7075\u654f\uff1a\u53cc\u8272\u63a2\u9488\uff0c\u7075\u654f\u5ea6\u9ad8\u3002<\/span><\/p>\n

2<\/span>.<\/span>\u00a0<\/span>\u7a33\u5b9a\uff1a\u5168\u7a0b\u95ed\u7ba1\u64cd\u4f5c\uff0c\u65e0\u4ea4\u53c9\u6c61\u67d3\u3002<\/span><\/p>\n

3<\/span>. <\/span>\u53ef\u9760\uff1a\u542b\u5185\u53c2\u57fa\u56e0\uff0c\u907f\u514d\u5047\u9634\u6027\u3002<\/span><\/p>\n

4<\/span>. <\/span>\u65b9\u4fbf\uff1a\u64cd\u4f5c\u7b80\u5355\uff0c\u65e0\u9700\u7535\u6cf3\u6b65\u9aa4\u3002<\/span><\/p>\n

5. <\/span>\u5b9a\u91cf\uff1a<\/span> \u4ee5<\/span><\/span>CT<\/span>\u503c\u5224\u65ad\uff0c\u53ef\u5b9a\u91cf\u68c0<\/span>\u6d4b<\/span>\u3002<\/span><\/p>\n

<\/p>\n

\u3010<\/span>\u8fd0\u8f93\u53ca\u4fdd\u5b58\u6761\u4ef6\u3011<\/span><\/p>\n

\u4f4e\u6e29\u51b7\u51bb\u8fd0\u8f93<\/span> \uff0c\u907f\u5149<\/span><\/span>-20\u2103<\/span>\u4fdd\u5b58<\/span> \uff0c\u6709\u6548\u671f<\/span><\/span>6<\/span>\u4e2a<\/span><\/span><\/p>\n

\u6708\u3002\u5f00\u76d6\u540e\uff0c<\/span><\/span>4\u2103<\/span>\u4fdd\u5b58\uff0c\u6709\u6548\u671f<\/span><\/span>1<\/span>\u5468<\/span><\/span>\u3002<\/span><\/span><\/p>\n

\u3010\u4ea7\u54c1\u7ec4\u5206\u3011<\/span><\/p>\n

\u00a0<\/span><\/p>\n\n\n\n\n\n\n\n\n\n
\n

\u7ec4<\/span>\u5206\u540d\u79f0<\/span><\/p>\n<\/td>\n

\n

BPT<\/span>5<\/span>0<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

Biowing<\/span>\u00ae<\/span>\u00a0<\/span>Specific<\/span>\u00a0<\/span>Reverse<\/span>\u00a0<\/span>Transcription<\/span>\u00a0<\/span>Primer<\/span><\/p>\n<\/td>\n

\n

2<\/span>0<\/span>\u00b5<\/span>L<\/span>*2<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

Biowing<\/span>\u00ae<\/span>qPCR<\/span>\u00a0<\/span>Mix<\/span><\/p>\n<\/td>\n

\n

312<\/span><\/span>\u00b5<\/span>L<\/span>*5<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

Biowing<\/span>\u00ae<\/span>Probe<\/span>\u00a0<\/span>qPCR<\/span>\u00a0<\/span>Primer<\/span><\/p>\n<\/td>\n

\n

90<\/span><\/span>\u00b5<\/span>L<\/span>*5<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

Positive<\/span>\u00a0<\/span>Control<\/span>\u00a0<\/span><\/p>\n<\/td>\n

\n

10<\/span>\u00b5<\/span>L<\/span>*5<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

Negative<\/span>\u00a0<\/span>Control<\/span><\/p>\n<\/td>\n

\n

10<\/span>\u00b5<\/span>L<\/span>*5<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

RNase<\/span>\u00a0<\/span>Free<\/span>\u00a0<\/span>Water<\/span><\/p>\n<\/td>\n

\n

200<\/span>\u00b5<\/span>L<\/span>*5<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6ce8\uff1a<\/span>1.\u672c\u8bd5\u5242\u76d2\u63d0\u4f9b\u8bd5\u5242\uff0c\u4f7f\u7528\u524d\u5148\u4f4e\u901f\u79bb\u5fc3\u3001\u540e\u5c0f\u5fc3\u5f00\u542f\u3002\u4f7f\u7528\u65f6\u8bf7\u4e0a\u4e0b\u8f7b\u67d4\u98a0\u5012\u5341\u6b21\u6df7\u5300\uff0c\u907f\u514d\u8d77\u6ce1\uff0c\u5e76\u4f4e\u901f\u77ed\u6682\u79bb<\/span>\u5fc3<\/span>\u540e\u4f7f\u7528\uff1b<\/span>2.\u4e3a\u4e86\u907f\u514d\u53cd\u590d\u51bb\u878d\uff0c10\u4eba\u4efd\/\u7ba1\u4f5c\u4e3a\u4e00\u4e2a\u5305\u88c5<\/span>\u3002<\/span><\/p>\n

\u3010\u64cd\u4f5c\u6b65\u9aa4\u3011<\/span><\/p>\n

1<\/span>. <\/span>\u9006\u8f6c\u5f55\u53cd\u5e94<\/span><\/p>\n

\u4f7f<\/span>\u7528<\/span>Trizol<\/span>\u6cd5\u62bd\u63d0\u7ec6\u80de<\/span>RNA<\/span>\u00a0<\/span>\uff0c<\/span>RNA<\/span>\u7eaf\u5ea6<\/span>A<\/span>260\/280<\/span>\u5728<\/span>1.9-2.0<\/span>\u4e4b\u95f4\u3002\u53d6<\/span>1<\/span>ug<\/span>\u00a0<\/span>RNA<\/span>\u4f7f\u7528<\/span>Thermo<\/span>\u9006\u8f6c\u5f55\u8bd5<\/span>\u5242<\/span>\u76d2<\/span>(\u8d27\u53f7\uff1a<\/span>K1622 <\/span>)<\/span>\uff0c<\/span>\u5c06<\/span>\u8bd5<\/span>\u5242<\/span>\u76d2<\/span>\u4e2d<\/span>\u7684<\/span> <\/span>Random \u00a0Primer <\/span>\u66ff<\/span>\u6362<\/span>\u6210<\/span><\/span>Biowing\u00ae Specific \u00a0Reverse<\/span>\u00a0Transcription<\/span>\u00a0<\/span>\u00a0<\/span>Primer<\/span>\u8fdb\u884c\u53cd\u8f6c\u5f55\u53cd\u5e94\u5f97\u5230<\/span>cDNA<\/span>\u3002<\/span><\/p>\n

\u00a0<\/span><\/p>\n

1. 1 <\/span>DNase<\/span>\u00a0<\/span>I<\/span>\u5904\u7406<\/span><\/p>\n

\u00a0<\/span><\/p>\n\n\n\n\n
\n

RNA<\/span>\u00a0<\/span>\u00a0<\/span>(<\/span>\u00b5<\/span>g<\/span>)<\/span><\/p>\n<\/td>\n

\n

DNase<\/span>\u00a0<\/span>Buffer<\/span>\u00a0\u00a0<\/span>(<\/span>\u00b5<\/span>L<\/span>)<\/span><\/p>\n<\/td>\n

\n

DNase<\/span>\u00a0\u2160( <\/span><\/span>\u00b5L)<\/span><\/p>\n<\/td>\n

\n

DEPC<\/span>\u00a0<\/span>\u6c34<\/span><\/span>(\u00b5<\/span>L<\/span>)<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

1<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n

\n

To<\/span>\u00a0<\/span>10<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u53cd\u5e94\u7a0b\u5e8f\uff1a<\/span><\/span>37\u2103 30<\/span>min<\/span>\u00a0<\/span>\uff1b\u52a0<\/span> <\/span>EDTA<\/span>\u00a01 <\/span>\u00b5<\/span>L<\/span>\u00a0<\/span>\u540e\uff0c<\/span><\/span>65\u2103 <\/span>\uff0c<\/span><\/span>10<\/span>min<\/span><\/p>\n

1<\/span>.2 \u00a0<\/span>\u9006\u8f6c\u5f55\u53cd\u5e94<\/span><\/p>\n

\u00a0<\/span><\/p>\n\n\n\n\n
\n

RNA( \u00b5<\/span>L<\/span>)<\/span><\/p>\n<\/td>\n

\n

Biowing<\/span>\u00ae<\/span>\u00a0<\/span>Specific<\/span>\u00a0<\/span>Reverse<\/span>\u00a0<\/span>Transcription Primer<\/span>( \u00b5<\/span>L)<\/span><\/p>\n<\/td>\n

\n

5\u00d7<\/span>Buffer<\/span>\u00a0<\/span>( \u00b5L)<\/span><\/p>\n<\/td>\n

\n

RT<\/span>(<\/span>\u00a0<\/span>\u00b5L)<\/span><\/p>\n<\/td>\n

\n

RI<\/span>( \u00b5<\/span>L)<\/span><\/p>\n<\/td>\n

\n

dNTP<\/span>(\u00b5<\/span>L<\/span>)<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

5.5<\/span><\/p>\n<\/td>\n

\n

0.<\/span>5<\/span><\/p>\n<\/td>\n

\n

2<\/span><\/p>\n<\/td>\n

\n

0.<\/span>5<\/span><\/p>\n<\/td>\n

\n

0.<\/span>5<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u53cd\u5e94<\/span><\/span>\u7a0b<\/span><\/span>\u5e8f\uff1a<\/span><\/span>20\u2103 10<\/span>min<\/span>\u00a0<\/span>\uff1b<\/span><\/span>42\u2103 1<\/span>h<\/span>\uff1b<\/span><\/span>72\u2103 5<\/span>min<\/span><\/p>\n

2. <\/span>qPCR<\/span>\u53cd\u5e94\u4f53\u7cfb\u53ca\u6761\u4ef6<\/span><\/p>\n

2. 1 <\/span>\u9633\u6027\u5bf9\u7167\u5904\u7406<\/span><\/p>\n

\u5c06\u9633\u6027\u5bf9\u7167<\/span><\/span>cDNA<\/span>\u8fdb\u884c<\/span><\/span>4<\/span><\/span>\u500d\u3001<\/span><\/span>8<\/span><\/span>\u500d\u7684\u68af\u5ea6\u7a00\u91ca\uff0c\u6bcf\u4e2a\u68af\u5ea6\u5efa\u8bae\u4e09\u91cd\u590d<\/span><\/span>\u3002<\/span><\/span><\/p>\n

2.2 <\/span>\u9634\u6027\u5bf9\u7167\u5904\u7406<\/span><\/p>\n

\u5c06\u9634\u6027\u5bf9\u7167<\/span><\/span>cDNA<\/span>\u8fdb\u884c<\/span><\/span>4<\/span><\/span>\u500d\u3001<\/span><\/span>8<\/span><\/span>\u500d\u7684\u68af\u5ea6\u7a00\u91ca\uff0c\u6bcf\u4e2a\u68af\u5ea6\u5efa\u8bae\u4e09\u91cd\u590d<\/span><\/span>\u3002<\/span><\/span><\/p>\n

2<\/span>.3 <\/span>\u6837\u672c\u5904\u7406<\/span><\/p>\n

\u5c06<\/span><\/span>\u5f85<\/span><\/span>\u6d4b\u6837\u672c<\/span><\/span>cDNA<\/span>\u8fdb\u884c<\/span><\/span>4<\/span><\/span>\u500d\u7a00\u91ca\u4f5c\u4e3a\u6d4b\u8bd5\u6d53\u5ea6\uff0c\u6bcf\u4e2a\u6837\u672c\u505a\u4e09\u91cd\u590d\u3002<\/span><\/span><\/p>\n

\u53cd\u5e94<\/span><\/span>\u4f53<\/span><\/span>\u7cfb<\/span> (\u4ee5<\/span><\/span>20\u00b5<\/span><\/span>L<\/span><\/span>\u4e3a\u4f8b<\/span>)<\/span><\/span>\u00a0<\/span><\/span><\/p>\n

\u00a0<\/span><\/p>\n\n\n\n\n\n\n\n\n\n
\n

\u7ec4<\/span><\/span>\u5206<\/span><\/span>\/<\/span>\u6837\u54c1\u7ba1<\/span><\/span><\/p>\n<\/td>\n

\n

\u5f85\u6d4b\u6837\u54c1<\/span><\/span>(\u00b5<\/span>L<\/span>)<\/span><\/p>\n<\/td>\n

\n

\u9633<\/span><\/span>\u6027\u5bf9\u7167\u7ba1<\/span><\/span>(\u00b5<\/span>L<\/span>)<\/span><\/p>\n<\/td>\n

\n

\u9634<\/span><\/span>\u6027\u5bf9\u7167\u7ba1<\/span><\/span>(\u00b5<\/span>L<\/span>)<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

Biowing<\/span>\u00ae<\/span>qPCR<\/span>\u00a0<\/span>Mix<\/span><\/p>\n<\/td>\n

\n

1<\/span>0<\/span><\/span>.<\/span>4<\/span><\/p>\n<\/td>\n

\n

1<\/span>0<\/span><\/span>.<\/span>4<\/span><\/p>\n<\/td>\n

\n

1<\/span>0<\/span><\/span>.<\/span>4<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

Biowing<\/span>\u00ae<\/span>Probe<\/span>\u00a0<\/span>qPCR<\/span>\u00a0<\/span>Primer<\/span><\/p>\n<\/td>\n

\n

3<\/span><\/p>\n<\/td>\n

\n

3<\/span><\/p>\n<\/td>\n

\n

3<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

S<\/span>ample<\/span><\/p>\n<\/td>\n

\n

3<\/span><\/p>\n<\/td>\n

\n

0<\/span><\/p>\n<\/td>\n

\n

0<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

Positive<\/span>\u00a0<\/span>Control<\/span><\/p>\n<\/td>\n

\n

0<\/span><\/p>\n<\/td>\n

\n

3<\/span><\/p>\n<\/td>\n

\n

0<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

Negative<\/span>\u00a0<\/span>Control<\/span><\/p>\n<\/td>\n

\n

0<\/span><\/p>\n<\/td>\n

\n

0<\/span><\/p>\n<\/td>\n

\n

3<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

RNase<\/span>\u00a0<\/span>Free<\/span>\u00a0<\/span>Water<\/span><\/p>\n<\/td>\n

\n

3.6<\/span><\/p>\n<\/td>\n

\n

3.6<\/span><\/p>\n<\/td>\n

\n

3.6<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

PCR<\/span><\/span>\u4eea<\/span><\/span>\u8bbe<\/span><\/span>\u7f6e<\/span> (\u4ee5<\/span><\/span>ABI<\/span><\/span>\u00a07500<\/span><\/span>\u4e3a\u4f8b<\/span>)<\/span><\/span>\u00a0<\/span><\/span><\/p>\n

\u00a0<\/span><\/p>\n\n\n\n\n\n\n\n\n\n
\n

\u6b65\u9aa4<\/span><\/p>\n<\/td>\n

\n

\u5faa<\/span>\u73af\u6570<\/span><\/p>\n<\/td>\n

\n

\u6e29<\/span>\u5ea6<\/span><\/p>\n<\/td>\n

\n

\u65f6\u95f4<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

1<\/span><\/p>\n<\/td>\n

\n

\u9884\u53d8\u6027<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n

\n

94\u2103<\/span><\/p>\n<\/td>\n

\n

5<\/span>\u00a0<\/span>min<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u00a0<\/span><\/p>\n

\u00a0<\/span><\/p>\n

2<\/span><\/p>\n<\/td>\n

\n

\u53d8\u6027<\/span><\/p>\n<\/td>\n

\n

\u00a0<\/span><\/p>\n

\u00a0<\/span><\/p>\n

40<\/span><\/p>\n<\/td>\n

\n

95\u2103<\/span><\/p>\n<\/td>\n

\n

15 s<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u9000\u706b<\/span><\/p>\n<\/td>\n

\n

58<\/span>\u2103<\/span><\/span><\/p>\n<\/td>\n

\n

45 <\/span>s<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u5ef6\u4f38<\/span><\/p>\n<\/td>\n

\n

7<\/span>2\u2103<\/span><\/p>\n<\/td>\n

\n

1 min<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u6e29\u5ea6\u9009\u62e9\uff1a<\/span><\/span>72\u2103<\/span>\u5ef6\u4f38\u65f6\u6536\u96c6\u8367\u5149\u4fe1<\/span><\/span>\u53f7<\/span><\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u901a\u9053\u9009\u62e9\uff1a\u6837\u672c\u68c0\u6d4b\u901a\u9053<\/span>–<\/span>FAM<\/span>\uff1b<\/span> \u5185\u53c2\u68c0\u6d4b\u901a\u9053<\/span><\/span>–<\/span>Cy<\/span>5<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/span><\/p>\n

\u3010\u57fa\u7ebf\u8bbe\u7f6e\u3011<\/span>(\u4ee5<\/span>ABI 7500<\/span>\u4e3a\u4f8b<\/span>)<\/span><\/p>\n

\u4e00<\/span>\u822c\u9ed8\u8ba4\u8d77\u59cb\u548c\u7ec8\u6b62<\/span>\u57fa\u7ebf\u4f4d\u7f6e<\/span>\u4e3a<\/span>3<\/span>– <\/span>15<\/span>\u4e2a\u5faa\u73af<\/span>\uff0c\u5e94\u6839\u636e\u5185\u53c2\u57fa\u56e0\u548c<\/span>TERT<\/span>\u57fa\u56e0\u5b9e\u9645\u6269\u589e\u66f2\u7ebf<\/span>\u624b\u52a8<\/span>\u5c06<\/span>\u57fa\u7ebf\u8d77<\/span>\u59cb<\/span>\u5faa<\/span>\u73af\u6570\u8c03\u6574\u4e3a\u6307\u6570\u589e\u957f\u671f\u4e4b\u524d\u5373\u53ef\u3002<\/span><\/p>\n

\u3010\u9608\u503c\u8bbe\u7f6e\u3011<\/span>(\u4ee5<\/span>ABI 7500<\/span>\u4e3a\u4f8b<\/span>)<\/span><\/p>\n

\u5185\u53c2\u9608\u503c\u8bbe\u5b9a\uff1a<\/span> \u4ee5\u9633\u6027\u5bf9\u7167<\/span><\/span>4<\/span>\u500d\u7a00\u91ca<\/span>cDNA<\/span>\u5b9a\u5185\u53c2\u57fa\u56e0\u6269\u589e\u66f2\u7ebf\u9608\u503c\uff0c<\/span> \u5185\u53c2\u57fa\u56e0<\/span><\/span>(<\/span>Cy<\/span>5)<\/span>\u521d\u59cb\u6d53\u5ea6\u7684<\/span>CT<\/span>\u503c<\/span>\u5b9a<\/span> <\/span>\u4e3a<\/span>1<\/span>7<\/span><\/span>\u00b13<\/span>\uff1b<\/span><\/p>\n

TERT<\/span>\u9608<\/span>\u503c<\/span>\u8bbe\u5b9a\uff1a<\/span> \u4ee5\u9633\u6027\u5bf9\u7167<\/span><\/span>4<\/span>\u500d\u7a00\u91ca<\/span>cDNA<\/span>\u5b9a<\/span>TERT<\/span>\u57fa\u56e0\u6269\u589e\u66f2\u7ebf\u9608\u503c\uff0c<\/span>TERT<\/span>\u57fa\u56e0<\/span>(<\/span>FAM<\/span>)<\/span>\u521d\u59cb\u6d53\u5ea6<\/span>CT<\/span>\u503c<\/span>\u00a0<\/span>\u7684\u5b9a\u4e3a<\/span>25\u00b13<\/span>\u3002<\/span><\/p>\n

\u3010\u7ed3\u679c\u5224\u8bfb\u3011<\/span><\/p>\n

\u00a0<\/span><\/p>\n

\u56fe<\/span>1<\/span>\u00a0<\/span>\u5de6<\/span>: <\/span>\u9633\u6027<\/span>\u5bf9\u7167<\/span>\u7684\u6269\u589e\u66f2\u7ebf\uff1b\u53f3<\/span>: <\/span>\u9634\u6027<\/span>\u5bf9\u7167<\/span>\u7684\u6269\u589e\u66f2\u7ebf<\/span><\/p>\n

(<\/span>\u84dd<\/span>\u8272\u4e3a<\/span>TERT<\/span>\u57fa\u56e0\u66f2\u7ebf<\/span>,<\/span>\u9ec4\u7eff\u8272\u4e3a\u5185\u53c2\u57fa\u56e0\u66f2\u7ebf<\/span>)<\/span><\/p>\n

1. <\/span>\u9633\u6027\u5bf9\u7167<\/span>4<\/span>\u500d<\/span>\u7a00\u91ca\u540e<\/span>TERT<\/span>\u57fa\u56e0<\/span>Ct<\/span>\u503c\u5728<\/span>25\u00b13<\/span>\uff0c\u5185\u53c2\u57fa\u56e0<\/span>Ct<\/span>\u503c\u5728<\/span>17\u00b13<\/span>\uff0c<\/span>8<\/span>\u500d\u7a00\u91ca\u540e<\/span>TERT<\/span>\u57fa\u56e0<\/span>Ct<\/span>\u503c\u5728<\/span>26\u00b13<\/span>\uff0c<\/span>\u5185\u53c2<\/span>\u57fa\u56e0<\/span>Ct<\/span>\u503c<\/span>\u5728<\/span>18\u00b13<\/span>\u00a0<\/span>\u3002<\/span>TERT<\/span>\u57fa\u56e0<\/span>\u548c\u5185\u53c2<\/span>\u57fa\u56e0<\/span>\u4e4b\u95f4<\/span>\u2206C<\/span><\/span>T<\/span>\u7ef4\u6301\u5728<\/span>9\u00b13<\/span>\uff0c\u4fdd\u6301\u7a33\u5b9a\u3002<\/span><\/p>\n

2. <\/span>\u9634<\/span>\u6027<\/span>\u5bf9\u7167\u7ecf<\/span>4<\/span>\u500d\u3001<\/span>8<\/span>\u500d\u68af\u5ea6\u7a00\u91ca\u540e\uff0c<\/span>TERT<\/span>\u57fa\u56e0<\/span>Ct<\/span>\u503c<\/span>\u226535 \u00a0<\/span>(\u6216\u68c0\u6d4b\u4e0d\u5230) \uff0c\u5185\u53c2\u57fa\u56e0<\/span>Ct<\/span>\u503c\u4ecd\u5728<\/span>1<\/span>8<\/span><\/span>\u00b13<\/span>\u4e4b\u95f4\uff0c\u5224<\/span>\u5b9a\u4e3a<\/span>\u7aef\u7c92\u9176\u6d3b\u6027\u4e3a\u9634\u6027\u3002<\/span><\/p>\n

3. <\/span>\u7ed3\u679c\u8bf4\u660e<\/span><\/p>\n

\u82e5\u5f85\u6d4b\u6837\u672c\u5185<\/span>\u53c2<\/span>\u57fa\u56e0<\/span>Ct<\/span>\u503c\u5728<\/span>17<\/span>\u00b13<\/span>\u4e4b\u95f4\uff0c<\/span>TERT<\/span>\u57fa\u56e0<\/span>Ct<\/span>\u503c\uff1c<\/span>35<\/span>\u4e14\u6269\u589e\u66f2\u7ebf\u6b63\u5e38\uff0c\u5219\u6837\u672c\u7aef\u7c92\u9176\u6d3b\u6027<\/span>\u4e3a\u9633\u6027\u3002<\/span><\/p>\n

\u82e5\u5f85\u6d4b\u6837\u672c\u5185\u53c2\u57fa\u56e0<\/span>Ct<\/span>\u503c\u5728<\/span>17<\/span>\u00b13<\/span>\u4e4b\u95f4\uff0c<\/span>TERT<\/span>\u57fa\u56e0<\/span>Ct<\/span>\u503c<\/span>\u226535<\/span>\u4e14\u65e0\u660e\u663e\u7684\u6269\u589e\u66f2\u7ebf<\/span>\uff0c<\/span>\u5219\u6837\u672c\u65e0\u7aef\u7c92<\/span>\u9176\u6d3b<\/span>\u6027\u3002<\/span><\/p>\n

\u3010\u8bf4\u660e\u3011<\/span><\/p>\n

1.<\/span>\u00a0<\/span>\u5f85\u6d4b\u6837\u672c<\/span>cDNA<\/span>\u00a0<\/span>4<\/span><\/span>\u500d\u7a00\u91ca\u4f5c\u4e3a\u6a21\u677f\uff0c\u4e09\u6b21\u6280\u672f\u91cd\u590d\uff0c\u4e0d\u8bbe\u7f6e\u68af\u5ea6\u7a00\u91ca\uff1b\u9633\u6027\u5bf9\u7167\u3001\u9634\u6027\u5bf9\u7167\u8fdb\u884c<\/span>4<\/span><\/span>\u500d\u3001<\/span>8<\/span>\u500d<\/span>\u68af\u5ea6\u7a00\u91ca\uff0c\u5efa\u8bae\u5206\u522b\u505a\u4e09\u91cd\u590d\uff1b\u907f\u514d\u5076\u7136\u8bef\u5dee\u7684\u4ea7\u751f\u3002<\/span><\/p>\n

2. <\/span>\u5982\u679c\u9633\u6027<\/span><\/span>\/<\/span>\u9634\u6027\u5bf9\u7167<\/span><\/span>4<\/span><\/span>\u500d\u7a00\u91ca\u540e\u5185\u53c2\u57fa\u56e0<\/span><\/span>Ct<\/span>\u503c\uff1e<\/span><\/span>20<\/span>\uff0c\u8868\u660e\u53c2\u8003\u54c1\u6709\u964d\u89e3\u6216\u8005\u8bd5\u5242\u76d2\u6269\u589e\u6548\u7387\u4e0b\u964d<\/span><\/span>\u3002<\/span><\/span><\/p>\n

3. <\/span>\u5982\u679c\u6240\u6709\u6837\u672c<\/span><\/span>4<\/span><\/span>\u500d\u7a00\u91ca\u540e\u5185\u53c2\u57fa\u56e0<\/span><\/span>Ct<\/span>\u503c\uff1e<\/span><\/span>20<\/span>\uff0c\u8868\u660e\u8bd5\u5242\u76d2\u7684\u6269\u589e\u6548\u7387\u4e0b\u964d\u3002<\/span><\/span><\/p>\n

4. <\/span>\u5982\u679c\u9633\u6027\u5bf9\u7167<\/span><\/span>4<\/span><\/span>\u500d\u7a00\u91ca\u540e<\/span><\/span>TERT<\/span>\u57fa\u56e0<\/span><\/span>Ct<\/span>\u503c\uff1e<\/span><\/span>28 <\/span>\uff0c\u8868\u660e<\/span><\/span>TERT<\/span>\u57fa\u56e0\u68c0\u6d4b\u7cfb\u7edf\u5931\u6548\u3002<\/span><\/span><\/p>\n

\u00a0<\/span><\/p>\n

\u00a0<\/span><\/p>\n

\u5982\u6709\u6280\u672f\u95ee<\/span>\u9898<\/span>\uff0c\u8bf7\u7535\u8bdd\u54a8\u8be2\uff1a<\/span>021-33559491<\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u672c\u4ea7\u54c1\u8bd5\u5242KIT\u91c7\u7528\u53cc\u8272\u8367\u5149qPCR\uff08TaqMan\u63a2\u9488\u6cd5\uff09\u68c0\u6d4b\u7aef\u7c92\u9176\u50ac\u5316\u4e9a\u57faTERT\u57fa\u56e0\u3002\u901a\u8fc7\u63d0\u53d6\u7ec6\u80deRNA\uff0c\u5229\u7528\u7279\u5f02\u6027\u9006\u8f6c\u5f55\u5f15\u7269\u8fdb\u884c\u9006\u8f6c\u5f55\u53cd\u5e94\uff0c\u4ee5cDNA\u4e3a\u6a21\u677f\u5728\u5355\u7ba1\u4e2d\u5229\u7528\u591a\u91cd\u8367\u5149\u5b9a\u91cfPCR\u8fdb\u884c\u6269\u589e\u53cd\u5e94\uff08\u53cc\u91cd\u63a2\u9488\uff1aFAM\u3001Cy5\uff09\u3002\u9009\u7528293T\u7ec6\u80de\u4e3a\u9633\u6027\u5bf9\u7167\u4ee5\u53caMRC-5\u7ec6\u80de\u4e3a\u9634\u6027\u5bf9\u7167\uff0c\u901a\u8fc7\u2206\u2206CT\u6cd5\u68c0\u6d4b\u6837\u672c\u4e2dTERT\u57fa\u56e0\u7684\u76f8\u5bf9\u8868\u8fbe\u91cf\u3002<\/p>\n","protected":false},"featured_media":769,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[20,21],"product_tag":[],"class_list":{"0":"post-602","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-jsfw","7":"product_cat-xbjyfw","9":"first","10":"instock","11":"shipping-taxable","12":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/product\/602","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/comments?post=602"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/media\/769"}],"wp:attachment":[{"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/media?parent=602"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/product_brand?post=602"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/product_cat?post=602"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.biowing.com.cn\/index.php\/wp-json\/wp\/v2\/product_tag?post=602"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}