{"id":613,"date":"2025-04-24T15:16:17","date_gmt":"2025-04-24T07:16:17","guid":{"rendered":"https:\/\/www.biowing.com.cn\/?post_type=product&p=613"},"modified":"2025-07-15T18:09:04","modified_gmt":"2025-07-15T10:09:04","slug":"cgfwgtyqdsnpfxccx","status":"publish","type":"product","link":"https:\/\/www.biowing.com.cn\/index.php\/product\/cgfwgtyqdsnpfxccx\/","title":{"rendered":"\u591a\u91cd\u957f\u7247\u6bb5\u6355\u83b7\u6d4b\u5e8f"},"content":{"rendered":"

\u591a\u91cd\u957f\u7247\u6bb5<\/span>PCR<\/span>\u6253\u65ad\u5efa\u5e93\u6280\u672f\u5e94\u7528\u4e8e<\/span>QTL<\/span>\u7cbe\u7ec6\u5b9a\u4f4d\uff0c\u5bf9\u5c11\u91cf\u8fd1\u7b49\u57fa\u56e0\u7cfb\u6837\u672c\u8fdb\u884c\u8f83\u5927\u533a\u95f4\uff08<\/span>50-200kb<\/span>\uff09\u7684\u5e8f\u5217\u5206\u6790\uff0c\u89e3\u51b3\u52a0\u5bc6\u6807\u8bb0\u65e0\u6cd5\u627e\u5230\u591a\u6001\u6027\u6807\u8bb0\u7684\u95ee\u9898\uff0c\u53ef\u5bf9\u76ee\u6807\u533a\u57df\u5185\u7684\u5019\u9009\u57fa\u56e0\u8fdb\u884c\u5e8f\u5217\u5206\u6790\uff0c\u6316\u6398\u529f\u80fd<\/span>SNP<\/span>\uff0c\u4e3a\u5f00\u53d1\u529f\u80fd\u5206\u5b50\u6807\u8bb0\u63d0\u4f9b\u652f\u6301\u3002\u800c\u76f4\u63a5\u4e00\u4ee3\u6d4b\u5e8f\u6210\u672c\u76f8\u5bf9\u8f83\u9ad8\uff0c\u73b0\u6709\u7684\u9ad8\u901a\u91cf\u5e8f\u5217\u5206\u6790\u6280\u672f\uff08\u6269\u589e\u5b50\u6d4b\u5e8f\u3001\u6db2\u76f8\u6355\u83b7\u7b49\uff09\u5bf9<\/span>50-200kb<\/span>\u533a\u95f4\u7684\u91cd\u6d4b\u5e8f\u65f6\u7531\u4e8e\u6837\u672c\u91cf\u901a\u5e38\u6bd4\u8f83\u5c0f\uff0c\u5e73\u5747\u5230\u6bcf\u4e2a\u6837\u672c\u7684\u6210\u672c\u4e5f\u76f8\u5bf9\u8f83\u9ad8\u3002\u4e3a\u4e86\u89e3\u51b3\u8fd9\u4e00\u95ee\u9898\uff0c\u4e0a\u6d77\u7ffc\u548c\u751f\u7269\u57fa\u4e8e\u591a\u91cd<\/span>PCR<\/span>\u6355\u83b7\u6280\u672f\uff0c\u81ea\u4e3b\u7814\u53d1\u4e86\u957f\u7247\u6bb5<\/span>PCR<\/span>\u6253\u65ad\u5efa\u5e93\u6280\u672f\uff0c\u5f88\u5927\u7a0b\u5ea6\u4e0a\u964d\u4f4e\u4e86\u5bf9<\/span>50-200kb<\/span>\u76ee\u6807\u533a\u95f4\u6355\u83b7\u5efa\u5e93\u7684\u6210\u672c\u3002<\/span><\/p>\n

\u5e94\u7528\u65b9\u5411:<\/strong><\/p>\n

\u519c\u53e3\uff1a<\/span>\u5168\u57fa\u56e0\u7ec4 SNP \u57fa\u56e0\u578b\u5206\u6790\uff1bQTL \u5b9a\u4f4d\u53ca\u57fa\u56e0\u5b9a\u4f4d\uff1b\u53d1\u73b0\u4f18\u826f\u7b49\u4f4d\u53d8\u5f02\uff1b\u5f00\u53d1\u529f\u80fd\u6807\u8bb0\uff1b\u5b9a\u5236\u80b2\u79cd Panel\uff1b\u5206\u5b50\u6807\u8bb0\u8f85\u52a9\u9009\u62e9\u80b2\u79cd\uff1b\u9057\u4f20\u6750\u6599\u524d\u666f\u53ca\u80cc\u666f\u9009\u62e9\uff1b\u5168\u57fa\u56e0\u7ec4\u9009\u62e9\uff1b\u4eb2\u7f18\u5173\u7cfb\u9274\u5b9a\u3001DNA \u6307\u7eb9\u56fe\u8c31\u3001\u54c1\u79cd\u9274\u5b9a\uff1b\u9057\u4f20\u591a\u6837\u6027\u8bc4\u4ef7\uff1b\u7fa4\u4f53\u9057\u4f20\u7ed3\u6784\u5206\u6790\u3002<\/span><\/p>\n

\u6280\u672f\u539f\u7406<\/strong><\/p>\n

\u4e0a\u6d77\u7ffc\u548c\u751f\u7269\u57fa\u4e8e\u81ea\u4e3b\u77e5\u8bc6\u4ea7\u6743\u7684\u591a\u91cd<\/span>PCR<\/span>\u6355\u83b7\u6280\u672f\uff0c\u9488\u5bf9\u8f83\u5927\u76ee\u6807\u533a\u95f4\uff0c\u81ea\u4e3b\u7814\u53d1\u4e86\u591a\u91cd\u957f\u7247\u6bb5<\/span>PCR<\/span>\u6253\u65ad\u5efa\u5e93\u6280\u672f\u3002<\/span>50-200kb<\/span>\u7684\u76ee\u6807\u533a\u95f4\u5e38\u89c4\u591a\u91cd<\/span>PCR<\/span>\u6355\u83b7\u5efa\u5e93\u9700\u8981<\/span>250<\/span>\u91cd<\/span>-1000<\/span>\u91cd\u6269\u589e\u53cd\u5e94\uff0c\u5f15\u7269\u8d39\u7528\u6602\u8d35\u3002\u591a\u91cd\u957f\u7247\u6bb5<\/span>PCR<\/span>\u6253\u65ad\u5efa\u5e93\u6280\u672f\uff0c\u5355\u4e2a\u6269\u589e\u533a\u95f4\u4e3a<\/span>3kb<\/span>\uff0c\u6709\u6548\u533a\u95f4\u4e3a<\/span>2.5kb<\/span>\uff0c<\/span>0.5kb<\/span>\u7684\u7247\u6bb5\u662f\u4e3a\u4e86\u6ee1\u8db3\u6269\u589e\u7247\u6bb5\u7684\u91cd\u53e0\uff0c\u4fdd\u8bc1\u6574\u4f53\u8986\u76d6\u5ea6\u3002<\/span>50-200kb<\/span>\u533a\u95f4\u53ea\u9700\u8981\u5408\u6210\u7684\u5f15\u7269\u964d\u4f4e\u4e3a<\/span>20<\/span>\u5bf9<\/span>-80<\/span>\u5bf9\uff0c\u964d\u4f4e\u4e86\u6355\u83b7\u5efa\u5e93\u7684\u6210\u672c\u3002\u591a\u91cd\u957f\u7247\u6bb5<\/span>PCR<\/span>\u6355\u83b7\u76ee\u6807\u7247\u6bb5\u4ee5\u540e\uff0c\u8fdb\u884c\u8d85\u58f0\u6253\u65ad\uff0c\u5e76\u5efa\u7acb<\/span>DNA<\/span>\u6587\u5e93\uff0c\u5728\u4e3b\u6d41\u4e8c\u4ee3\u6d4b\u5e8f\u5e73\u53f0<\/span>Illumina X10<\/span>\u4e0a\u673a\u6d4b\u5e8f\uff0c\u6570\u636e\u4e0b\u673a\u4e4b\u540e\u901a\u8fc7\u751f\u4fe1\u5206\u6790\uff0c\u83b7\u53d6\u76ee\u6807\u533a\u95f4\u6838\u82f7\u9178\u53d8\u5f02\u4fe1\u606f\u3002<\/span><\/p>\n

\u591a\u91cd\u957f\u7247\u6bb5\u6355\u83b7\u6d4b\u5e8f<\/p>\n

\u6280\u672f\u8def\u7ebf<\/p>\n

\"\"<\/p>\n

    \n
  1. \u9ad8\u540c\u6e90\u533a\u6bb5SNP\u5206\u578b\/\u91cd\u6d4b\u5e8f<\/li>\n<\/ol>\n

    \u9ad8\u540c\u6e90\u533a\u6bb5\u5728\u65e0\u6cd5\u83b7\u5f97\u7279\u5f02\u6027PCR\u4ea7\u7269\u7684\u60c5\u51b5\u4e0b\uff0c\u6d4b\u5e8f\u83b7\u5f97\u7684\u5e8f\u5217\u662f\u591a\u4e2a\u5206\u5b50\u7684\u6df7\u5408\u7ed3\u679c\u3002\u56e0\u6b64\uff0c\u4eba\u7c7b\u9ad8\u540c\u6e90\u57fa\u56e0SNP\u5206\u578b\u3001\u591a\u500d\u4f53\u7269\u79cdSNP\u5206\u578b\uff0c\u7ed9\u9057\u4f20\u5b66\u5e26\u6765\u6311\u6218\u3002\u4f7f\u7528\u957fPCR\u6355\u83b7\u5176\u5355\u4e00\u7247\u6bb5\uff0c\u80fd\u591f\u6e05\u6670\u6709\u6548\u7684\u63d0\u51fa\u540c\u6e90\u7247\u6bb5\u7684\u5e72\u6270\u3002<\/p>\n

    \u63cf\u8ff0<\/p>\n

    \u591a\u91cd\u957fPCR\u5efa\u5e93\u6280\u672f\u80fd\u76f4\u63a5\u8de8\u8fc7\u9ad8\u540c\u6e90\u533a\u8bbe\u8ba1\u5f15\u7269\uff0c\u8bfb\u5230\u76ee\u7684\u7247\u6bb5\u6838\u82f7\u9178\u7684\u5b8c\u6574\u5e8f\u5217\uff0c\u53ef\u4f5c\u4e3aHLA\u5206\u578b\u6700\u76f4\u63a5\u3001\u6700\u7cbe\u786e\u3001\u6700\u53ef\u9760\u7684\u65b9\u6cd5\u3002\u53ef\u7528\u4e8e\u5bf9\u65b0\u53d1\u73b0\u7684HLA\u7279\u5f02\u6027\u8fdb\u884cDNA\u5e8f\u5217\u5206\u6790\u3002<\/p>\n

    \u5bf9\u5927\u7247\u6bb5p450\u57fa\u56e0\u6d4b\u5e8f\uff0c\u7ec6\u80de\u8272\u7d20P450\u57fa\u56e0\u662f\u5e9e\u5927\u3001\u590d\u6742\u7684\u8d85\u57fa\u56e0\u5bb6\u65cf\uff0c\u5e7f\u6cdb\u5b58\u5728\u4e8e\u52a8\u7269\u3001\u690d\u7269\u3001\u7ec6\u83cc\u771f\u83cc\u7b49\u7ec6\u80de\u5185”\uff0c\u901a\u8fc7\u591a\u91cd\u957f\u7247\u6bb5PCR\u5efa\u5e93\u6280\u672f\u5bf9P450\u57fa\u56e0\u8fdb\u884c\u6d4b\u5e8f\u5206\u6790\uff0c\u53ef\u7814\u7a76\u5176\u57fa\u56e0\u7684\u591a\u6001\u6027\u3002<\/p>\n

    \u7ebf\u7c92\u4f53\u4e0e\u67d3\u8272\u4f53DNA\u6709\u8f83\u5f3a\u7684\u540c\u6e90\u6027\uff0c\u901a\u8fc7\u4f7f\u7528\u957f\u7247\u6bb5PCR\u6355\u83b7\u5efa\u5e93\u6280\u672f\u68c0\u6d4b\u7ebf\u7c92\u4f53DNA\u3002<\/p>\n

    \u5e94\u7528\u65b9\u5411\uff1a\u9ad8\u540c\u6e90\u533a\u6bb5\u91cd\u6d4b\u5e8f\u3001HLA\u5206\u578b\u3001\u5927\u7247\u6bb5p450\u57fa\u56e0\u6d4b\u5e8f\u3001\u7ebf\u7c92\u4f53\u6d4b\u5e8f<\/p>\n

      \n
    1. \u5c11\u91cf\u6837\u672c\u548c\u957f\u7247\u6bb5\u6355\u83b7\u6d4b\u5e8f<\/li>\n<\/ol>\n

      \u9776\u5411\u6355\u83b7\u6d4b\u5e8f\u4e2d\uff0c\u91c7\u7528\u82af\u7247\u6355\u83b7\u5bb9\u6613\u5e26\u6765\u8f83\u9ad8\u7684\u57fa\u7840\u8bbe\u8ba1\u4e0e\u5408\u6210\u6210\u672c\uff0c\u5728\u5c11\u91cf\u6837\u672c\u6d4b\u5e8f\u4e2d\u96be\u4ee5\u644a\u8584\u3002\u800c\u91c7\u7528\u957f\u7247\u6bb5\u6355\u83b7\uff0c\u4ec5\u9700\u4e00\u5bf9\u5f15\u7269\u7684\u8bbe\u8ba1\u4e0e\u5408\u6210\u8d39\u7528\uff0c\u7ecf\u6d4e\u9ad8\u6548\u3002<\/p>\n

      \u63cf\u8ff0<\/p>\n

      \u591a\u91cd\u957fPCR\u5efa\u5e93\u6280\u672f\uff0c\u9002\u7528\u4e8e\u5c11\u91cf\u6837\u672c\u7684\u8fde\u7eed\u6216\u4e0d\u8fde\u7eed50-200kb\u76ee\u7684\u533a\u95f4\u8fdb\u884c\u9ad8\u6df1\u5ea6\u76ee\u6807\u533a\u57df\u533a\u57df\u91cd\u6d4b\u5e8f\uff0c\u83b7\u53d6\u53d8\u5f02\u4fe1\u606f\uff08SNP\u548cIndel\uff09\u3002\u4e3b\u8981\u7528\u4e8eQTL\u533a\u95f4\u5185\u6807\u5019\u9009\u533a\u6bb5\u7684\u91cd\u6d4b\u5e8f\u3002<\/p>\n

      \u57fa\u56e0\u7f16\u8f91\u65f6\uff0c\u5f53\u57fa\u56e0\u7ec4\u5e8f\u5217\u4e2d\u95f4\u90e8\u5206\u53d1\u751f\u8f83\u5927\u53d8\u5316\u65e0\u6cd5\u5728\u6b64\u8303\u56f4\u8bbe\u8ba1\u5f15\u7269\u65f6\uff0c\u4f7f\u7528\u957fPCR\u5efa\u5e93\u6280\u672f\u8de8\u8fc7\u53d8\u5316\u533a\u6bb5\u8bbe\u8ba1\u5f15\u7269\uff0c\u5c06\u53ef\u80fd\u53d1\u751f\u53d8\u5316\u7684\u533a\u57df\u8fdb\u884c\u5168\u8986\u76d6\u6269\u589e\u6d4b\u5e8f\uff1b\u53ef\u7528\u4e8e\u57fa\u56e0\u7f16\u8f91\u548c\u57fa\u56e0\u6572\u5165\u7684\u80cc\u666f\u9274\u5b9a\u3002<\/p>\n

      \u7531\u4e8e\u4f20\u7edf\u68c0\u6d4b\u65b9\u6cd5\u7684\u9650\u5236\uff0c\u5355\u4e2a\u6216\u591a\u4e2a\u5916\u663e\u5b50\u5927\u7247\u6bb5\u7684\u7f3a\u5931\/\u91cd\u590d\u5f80\u5f80\u88ab\u5ffd\u89c6\u3002\u56e0\u6b64\uff0c\u4f7f\u7528\u591a\u91cd\u957f\u7247\u6bb5PCR\u6355\u83b7\u5efa\u5e93\u6280\u672f\u53ef\u5c06\u5305\u62ec\u5185\u542b\u5b50\u548c\u5916\u663e\u5b50\u5728\u5185\u7684\u5168\u90e8\u5e8f\u5217\u8fdb\u884c\u6d4b\u5b9a\uff0c\u4f5c\u4e3a\u4e00\u79cd\u68c0\u6d4b\u57fa\u56e0\u7684\u591a\u5916\u663e\u5b50\u5927\u7247\u6bb5\u7f3a\u5931\/\u91cd\u590d\u7684\u65b9\u6cd5\u3002<\/p>\n

      \u5e94\u7528\u65b9\u5411\uff1aQTL\u533a\u95f4\u5185\u6807\u5019\u9009\u533a\u6bb5\u7684\u91cd\u6d4b\u5e8f\u3001\u5c11\u91cf\u6837\u672c50-200kb\u533a\u95f4\u91cd\u6d4b\u5e8f\u3001\u5927\u7247\u6bb5\u7f3a\u5931\u7684\u68c0\u6d4b<\/p>\n

       <\/p>\n

      \u7ffc\u548c\u7279\u8272\uff1a<\/p>\n

      1.\u591a\u91cd\u957fPCR\u6355\u83b7\u5efa\u5e93\u6280\u672f\uff0c\u5b8c\u7f8e\u7ed3\u5408\u4e86\u957fPCR\u548c\u591a\u91cdPCR\uff0c\u5438\u6536\u4e86\u4e24\u79cd\u65b9\u6cd5\u7684\u4f18\u70b9\uff0c\u9996\u6b21\u6311\u6218\u5e76\u5efa\u7acb\u4e86\u4e00\u79cd\u65b0\u7684\u9776\u5411\u6355\u83b7\u5efa\u5e93\u4e8c\u4ee3\u6d4b\u5e8f\u7684\u65b9\u6848\u3002<\/strong><\/p>\n

      2.\u80fd\u5b8c\u6210\u6269\u589e\u5927\u4e8e5kb\u7684DNA\u7247\u6bb5\uff1b<\/strong><\/p>\n

      3.\u5e73\u5747\u8986\u76d6\u5ea6\u8fbe\u523099%\u4ee5\u4e0a\uff1b<\/strong><\/p>\n

      4.\u80fd\u6355\u83b750-200kb\u533a\u6bb5\uff1b<\/strong><\/p>\n

       <\/p>\n

       <\/p>\n","protected":false},"excerpt":{"rendered":"

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