{"id":686,"date":"2025-05-29T11:23:14","date_gmt":"2025-05-29T03:23:14","guid":{"rendered":"https:\/\/www.biowing.com.cn\/?post_type=product&p=686"},"modified":"2025-07-07T13:59:01","modified_gmt":"2025-07-07T05:59:01","slug":"qtsjhdlcdcdcj","status":"publish","type":"product","link":"https:\/\/www.biowing.com.cn\/index.php\/product\/qtsjhdlcdcdcj\/","title":{"rendered":"\u7aef\u7c92\u7edd\u5bf9\u957f\u5ea6-\u68c0\u6d4b\u8bd5\u5242\u76d2"},"content":{"rendered":"

\u7aef\u7c92\u7edd\u5bf9\u957f\u5ea6-\u68c0\u6d4b\u8bd5\u5242\u76d2<\/p>\n

\u3010\u4ea7\u54c1\u89c4\u683c\u3011<\/p>\n

TL50-02 50*3 Reactions<\/p>\n

\u3010\u4ea7\u54c1\u8bf4\u660e\u3011<\/p>\n

\u7aef\u7c92\uff08Telomeres\uff09\uff1a\u662f\u771f\u6838\u751f\u7269\u7ebf\u6027\u67d3\u8272\u4f53\u672b\u7aef\u7684\u975e\u7f16\u7801\u4e32\u8054\u91cd\u590d\u6027(TTAGGG)n\u9635\u5217\uff0c\u7aef\u7c92\u957f\u5ea6\u7684\u53d8\u5316\uff0c\u4e0e\u8870\u8001\u3001\u764c\u75c7\u3001\u6216\u795e\u7ecf\u9000\u884c\u6027\u7b49\u591a\u79cd\u75be\u75c5\u76f8\u5173\u3002qPCR\u6cd5\u662f\u68c0\u6d4b\u7aef\u7c92\u957f\u5ea6\u7684\u7ecf\u5178\u6280\u672f\uff0c\u7528\u65f6\u77ed\uff0c\u64cd\u4f5c\u7b80\u5355\uff0c\u7ed3\u679c\u51c6\u786e\uff0c\u9002\u7528\u4e8e\u6279\u91cf\u6837\u672c\u68c0\u6d4b\u3002<\/p>\n

\u672c\u4ea7\u54c1\u91c7\u7528\u53cc\u8272\u8367\u5149 qPCR\uff08SYBRgreen+VIC\uff09\u68c0\u6d4b\u7aef\u7c92\u957f\u5ea6\uff0c\u68c0\u6d4b\u5bf9\u8c61\u4e3a\u8840\u6db2\u3001\u7ec6\u80deDNA\u3002\u76f8\u5173\u4fe1\u606f\u89c1\u53c2\u8003\u6587\u732e\u30101\u3011\u3002\u7aef\u7c92\u957f\u5ea6\u6570\u636e\u5448\u73b0\u4e24\u79cd\u65b9\u5f0f\uff1a\u76f8\u5bf9\u957f\u5ea6\uff08T\/S\uff09\u548c\u5e73\u5747\u7aef\u7c92\u957f\u5ea6(bp)\u3002<\/p>\n

\"\u56fe\u72472\" \u8be5\u8bd5\u5242\u76d2\u5177\u6709\u4ee5\u4e0b\u7279\u70b9\uff1a<\/p>\n

1.\u65b9\u4fbf\uff1a\u64cd\u4f5c\u7b80\u5355\u3002<\/p>\n

2.\u53ef\u9760\uff1a\u7aef\u7c92\u4e0e\u5185\u53c2\u5355\u7ba1\u68c0\u6d4b\uff0c\u51cf\u5c11\u5b54\u95f4<\/p>\n

\u5e72\u6270\u3002\u5185\u53c2\u57fa\u56e0\u4e3a\u591a\u62f7\u8d1d\uff0c\u4e0e\u7aef\u7c92\u91cf\u5dee\u964d\u4f4e\u3002<\/p>\n

3.\u51c6\u786e\uff1a\u5f15\u5165\u5df2\u77e5\u7aef\u7c92\u7edd\u5bf9\u957f\u5ea6\u7684\u6807\u51c6\u54c1\u548c<\/p>\n

\u6821\u6b63\u54c1\uff0c\u4e00\u4e2a\u7528\u4e8e\u6784\u5efa\u6807\u51c6\u66f2\u7ebf\uff0c\u4e00\u4e2a\u7528\u4e8e\u6821\u6b63\uff0c\u53ef\u540c\u65f6\u83b7\u5f97\u6837\u54c1\u7684\u76f8\u5bf9\u957f\u5ea6\uff08T\/S\uff09\u548c\u7edd\u5bf9\u957f\u5ea6\u3002<\/p>\n

\u3010\u8fd0\u8f93\u53ca\u4fdd\u5b58\u6761\u4ef6\u3011<\/p>\n

\u4f4e\u6e29\u51b7\u51bb\u8fd0\u8f93\uff0c-20 \u2103\u4fdd\u5b58\uff0c\u6709\u6548\u671f 1\u5e74\uff0c\u51bb\u878d\u4e0d\u8d85\u8fc73\u6b21\u3002<\/p>\n

\u3010\u4ea7\u54c1\u7ec4\u5206\u3011<\/p>\n

1.<\/p>\n

\"\u8868\u683c<\/p>\n

Primer&Probe \u514812000 rpm\u79bb\u5fc35 min\uff0c\u6bcf\u7ba1\u518d\u7528775 \u00b5L RNase Free Water\u590d\u6eb6\u3002<\/p>\n

2. \u672c\u8bd5\u5242\u76d2\u63d0\u4f9b\u7684\u8bd5\u5242\uff0c\u4f7f\u7528\u524d\u8bf7\u4e0a\u4e0b\u8f7b\u67d4\u98a0\u5012\u5341\u6b21\u6df7\u5300\uff0c\u540e\u4f4e\u901f\u79bb\u5fc3\u3001\u5c0f\u5fc3\u5f00\u542f\u3002\u907f\u514d\u8d77\u6ce1\u3002\u5b9e\u9a8c\u65f6\uff0c\u64cd\u4f5c\u987b\u5168\u7a0b\u5728\u51b0\u4e0a\u8fdb\u884c\u3002<\/p>\n

3. \u5b9e\u9a8c\u9664\u4e0a\u8ff0\u7ec4\u5206\u5916\uff0c\u6807\u51c6\u54c1\u548c\u6821\u6b63\u54c1\u9700\u8981\u5728\u672c\u516c\u53f8\u5355\u72ec\u8d2d\u4e70(\u8d27\u53f7\uff1aTL3-S&C\uff09\uff0c\u5176\u4e2d\u6807\u51c6\u54c1\u7528\u4e8e\u6784\u5efa\u6807\u51c6\u66f2\u7ebf\uff0c\u6821\u6b63\u54c1\u7528\u4e8e\u83b7\u5f97\u6821\u6b63\u7cfb\u6570\uff0c\u6807\u51c6\u54c1\u548c\u6821\u6b63\u54c1\u5177\u4f53\u7b97\u6cd5\u89c1\u9644\u5f553\uff09\uff1bDNA\u7eaf\u5316\u6db2\u548cDNA\u7247\u6bb5\u7b5b\u9009\u529f\u80fd\u78c1\u73e0\u4e5f\u9700\u8981\u5728\u672c\u516c\u53f8\u5355\u72ec\u8d2d\u4e70\uff08\u8d27\u53f7\uff1aTL50-P\uff09\uff0c\u7528\u4e8e\u7eaf\u5316DNA\u3002<\/p>\n

\u3010\u64cd\u4f5c\u6b65\u9aa4\u3011<\/p>\n

1.\u6837\u54c1\u51c6\u5907\u53ca\u8d28\u63a7\uff08\u89c1\u9644\u5f551\uff09<\/p>\n

2.qPCR\u53cd\u5e94\u6db2\u7684\u51c6\u5907<\/p>\n

1.\u6839\u636e\u6240\u8981\u68c0\u6d4b\u6837\u54c1\u7684\u6570\u91cf\uff0c\u8ba1\u7b97\u6240\u9700\u53cd\u5e94\u5b54\u6570\uff0c\u6bcf\u4e2a\u6837\u672c\u505a3 \u4e2a\u91cd\u590d\u5b54\u3002<\/p>\n

\u53cd\u5e94\u5b54\u6570=\u6807\u51c6\u54c1\u00d7 3\u4e2a\u68af\u5ea6\u00d7 3+\uff08\u6821\u6b63\u54c1+\u6837\u672c\u6570\uff09\u00d7 3\uff08\u6807\u51c6\u66f2\u7ebf\u6cd5\uff09<\/strong><\/p>\n

\u6216\u53cd\u5e94\u5b54\u6570=\u6807\u51c6\u54c1\u00d7 3+ \u6837\u672c\u6570\u00d7 3\uff08\u25b3\u25b3Ct\u6cd5\uff09<\/strong><\/p>\n

2.\u6839\u636e\u53cd\u5e94\u5b54\u6570\u8ba1\u7b97\u6240\u9700\u7684 Mix \u603b\u91cf\uff08\u542b\u6709 1\u5b54\u7684\u52a0\u6837\u635f\u5931\u91cf\uff09\uff1a<\/p>\n

Mix =\uff08\u53cd\u5e94\u5b54\u6570+1\uff09\u00d7 25 \u00b5L<\/p>\n

3.\u5404\u8bd5\u5242\u653e\u5ba4\u6e29\u6761\u4ef6\u4e0b\u878d\u5316\uff0c\u5e76\u6839\u636e\u4e0b\u8868\u6240\u793a\u51c6\u5907 qPCR Mix\uff1a<\/p>\n

3.\u52a0\u6837<\/p>\n

\"\u8868\u683c<\/p>\n

    \n
  1. \u9707\u8361\u6df7\u5300 qPCR Mix\uff0c\u4f4e\u901f\u79bb\u5fc3\uff0c\u5c06\u7ba1\u76d6\u6b8b\u7559\u6db2\u4f53\u6536\u96c6\u81f3\u7ba1\u5e95\u3002<\/li>\n
  2. \u5411\u6bcf\u5b54\u53cd\u5e94\u7ba1\u4e2d\u5206\u88c525 \u00b5L qPCR Mix\u3002<\/li>\n
  3. \u5411\u5df2\u5206\u88c5\u8fc7 qPCR Mix \u7684\u53cd\u5e94\u7ba1\u4e2d\u52a0\u516515 \u00b5L\u77f3\u8721\u6cb9\u3002<\/li>\n
  4. \u5411\u53cd\u5e94\u7ba1\u4e2d\u7a7f\u8fc7\u77f3\u8721\u6cb9\u5c42\u52a0\u5165\u6837\u54c1\u540e\u77ed\u6682\u79bb\u5fc3\uff0c\u52a0\u6837\u793a\u4f8b\u5982\u4e0b\uff1a<\/li>\n<\/ol>\n

    \u6807\u51c6\u66f2\u7ebf\u6cd5\uff1a<\/p>\n

    \"\u8868\u683c<\/p>\n

    \u25b3\u25b3Ct\u6cd5\uff1a<\/p>\n

    \"\u8868\u683c<\/p>\n

    \u6ce8\uff1a\u76d6\u4e0a\u516b\u8054\u7ba1\u76d6\u6216\u8005\u5149\u5b66\u819c\u540e\u76d6\u4e0a\u53cd\u5e94\u7ba1\u76d6\u5b50\u6216\u8005\u8d34\u4e0a\u5149\u5b66\u819c\uff0c\u4e3a\u907f\u514d\u5f71\u54cd\u8367\u5149\u4fe1\u53f7\u8bfb\u53d6\uff0c\u8bf7\u6ce8\u610f\u4e0d\u8981\u5728\u7ba1\u76d6\u6216\u8005\u819c\u4e0a\u505a\u6807\u8bb0\uff0c\u6216\u8005\u7528\u522e\u677f\u53cd\u590d\u6469\u64e6\u3002<\/p>\n

    4.qPCR\u53cd\u5e94\u6761\u4ef6<\/p>\n

    PCR\u4eea\u8bbe\u7f6e\u4ee5 \u5b8f\u77f3SLAN 96S \u4e3a\u4f8b\uff0c\u5176\u4ed6\u578b\u53f7\u5b9a\u91cfPCR\u4eea\u5e94\u901a\u8fc7\u6807\u51c6\u54c1\u8fdb\u884c\u53c2\u6570\u6821\u6b63\u6216\u54a8\u8be2\u4e13\u4e1a\u6280\u672f\u4eba\u5458\u3002<\/strong><\/p>\n

    \u3010\u9608\u503c\u8bbe\u7f6e\u3011<\/p>\n

    \"\u8868\u683c<\/p>\n

    1. \u4ee5\u5b8f\u77f3SLAN-96S\u4e3a\u4f8b\uff0c\u6269\u589e\u66f2\u7ebf\u7b97\u6cd5\u9009\u62e9\u7edd\u5bf9\u8367\u5149\u503c\u6cd5\uff0c\u57fa\u7ebf\u8bbe\u7f6e\u57282-7\u4e2a\u5faa\u73af\u3002<\/p>\n

    2. \u9608\u503c\u7684\u8bbe\u5b9a\u8981\u4fdd\u8bc1\u6837\u672c\u53ca\u6807\u51c6\u54c1\u76843\u91cd\u590d\u7684\u25b3Ct\u57280.3\u4ee5\u5185\u3002<\/p>\n

    3. \u9608\u503c\u8bbe\u5b9a\uff1a\u5185\u53c2\u548c\u7aef\u7c92\u6269\u589e\u66f2\u7ebf\u9608\u503c\u62c9\u540c\u4e00\u6839\u7ebf\uff0c\u90fd\u5b9a\u4e3a700\uff1b40 ng \u6807\u51c6\u54c1\u7aef\u7c92\u7684Ct\u503c\u5b9a\u4e3a13\u00b11\uff0c\u5185\u53c2\u7684Ct\u503c\u5b9a\u4e3a18\u00b11\uff1b\u6807\u51c6\u54c1\u7aef\u7c92\u548c\u5185\u53c2\u7684\u25b3Ct\u4e3a5\u00b10.5\u3002<\/p>\n

    4. \u9608\u503c\u8bbe\u7f6e\u4ee5\u540e\uff0c\u5982\u4e0b\u56fe\u6240\u793a\uff0c\u6807\u51c6\u54c1 3\u4e2a\u6d53\u5ea6\u68af\u5ea6\u4e4b\u95f4\u5448\u73b0\u8f83\u597d\u7684Ct\u5dee\u5f02\uff0c\u7aef\u7c92\u901a\u9053\u548c\u5185\u53c2\u901a\u9053\u7684\u25b3Ct\u5747\u57280.8\u5de6\u53f3\u3002<\/p>\n

    \"\u5fae\u4fe1\u56fe\u7247_20231215090329\"<\/p>\n

    \u8bf4\u660e\uff1a<\/p>\n

    \u6839\u636e\u6807\u51c6\u54c1\u6807\u51c6\u66f2\u7ebf\uff0c\u82e5R2<\/sup>\uff1c0.99\uff0c\u8868\u660e\u64cd\u4f5c\u6709\u8bef\u3002<\/p>\n

    \u5907\u6ce8\uff1a<\/p>\n

    1. \u6807\u51c6\u54c1\u3001\u6821\u6b63\u54c1\u7684\u7edd\u5bf9\u957f\u5ea6\uff0c\u8bf7\u54a8\u8be2\u4e0a\u6d77\u7ffc\u548c\u5e94\u7528\u751f\u7269\u6280\u672f\u6709\u9650\u516c\u53f8\u3002\u4ea7\u54c1\u6279\u6b21\u4e0d\u540c\uff0c\u7edd\u5bf9\u957f\u5ea6\u4f1a\u6709\u5dee\u5f02\u3002<\/p>\n

    2. \u5982\u679c\u68c0\u6d4b\u6837\u672c\u4e3a\u8840\u6db2DNA\uff0c\u53ef\u4ee5\u5c06\u6240\u5f97\u7aef\u7c92\u76f8\u5bf9\u957f\u5ea6\u5728\u4e0a\u6d77\u7ffc\u548c\u5e94\u7528\u751f\u7269\u6280\u672f\u6709\u9650\u516c\u53f8\u6784\u5efa\u76845000\u4f8b\u4e2d\u56fd\u4eba\u7fa4\u6570\u636e\u5e93\u6bd4\u5bf9\uff0c\u8bc4\u4f30\u6837\u672c\u4e2a\u4f53\u7aef\u7c92\u7684\u76f8\u5bf9\u957f\u5ea6\uff1b\u82e5\u9009\u7528\u5176\u4ed6\u6837\u672c\uff0c\u5efa\u8bae\u81ea\u884c\u6784\u5efa\u6570\u636e\u5e93\u5e76\u505a\u8fdb\u4e00\u6b65\u5206\u6790\u3002<\/p>\n

    3. \u7aef\u7c92\u957f\u5ea6\u8ba1\u7b97\u65b9\u6cd5\u89c1\u9644\u5f553\u3002<\/p>\n

    \u9644\u5f55 1<\/p>\n

    \u6837\u672c\u53ca\u6807\u51c6\u54c1\u51c6\u5907\u548c\u8d28\u63a7<\/strong><\/p>\n

    1.\u6837\u54c1\u524d\u5904\u7406<\/p>\n

    1.1\u8840\u6db2\u6837\u672c<\/p>\n

    \u8840\u6db2\u6837\u672c\u5efa\u8bae\u5728\ufe6320\u2103\u4fdd\u5b58\uff0c\u672c\u8bd5\u5242\u76d2\u652f\u6301\u4ece\u6dcb\u5df4\u7ec6\u80de\u4e2d\u5206\u9009\u7684\u767d\u7ec6\u80de\u6216\u8840\u6db2\u7ecf\u7ea2\u7ec6\u80de\u88c2\u89e3\u6db2\u5904\u7406\u5206\u9009\u7684\u767d\u7ec6\u80de\u8fdb\u884c\u6838\u9178\u62bd\u63d0\uff0c\u5efa\u8bae\u91c7\u7528\u5929\u6839\u7684\u8840\u6db2\u57fa\u56e0\u7ec4DNA\u63d0\u53d6\u8bd5\u5242\u76d2(DP348)\u62bd\u63d0DNA\uff0c\u7528\u4e8e\u540e\u7eed\u7aef\u7c92\u957f\u5ea6\u7684\u68c0\u6d4b\u3002<\/p>\n

    1.2\u7ec6\u80de\u6837\u672c<\/p>\n

    \u5efa\u8bae\u91c7\u7528\u5929\u6839\u7684\u8840\u6db2\/\u7ec6\u80de\/\u7ec4\u7ec7\u57fa\u56e0\u7ec4DNA \u63d0\u53d6\u8bd5\u5242\u76d2(DP304)\u62bd\u63d0DNA\uff0c\u7528\u4e8e\u540e\u7eed\u7aef\u7c92\u957f\u5ea6\u7684\u68c0\u6d4b\u3002<\/p>\n

    1.3 \u553e\u6db2\u6837\u672c<\/p>\n

    \u553e\u6db2\u6837\u672c\u9700\u8981\u7ec6\u80de\u5206\u9009\u518d\u62bd\u63d0DNA\uff0c\u5206\u9009\u64cd\u4f5c\u8f83\u590d\u6742\uff0c\u5efa\u8bae\u54a8\u8be2\u672c\u516c\u53f8\u6280\u672f\u4eba\u5458\u3002<\/p>\n

    2.\u6837\u672c\u8d28\u91cf\u7684\u6807\u5316<\/p>\n

    \u7aef\u7c92\u957f\u5ea6\u7684\u6d4b\u5b9a\u4e0e\u6837\u672c\u8d28\u91cf\u76f8\u5173\u6027\u8f83\u5927\uff0cDNA\u8d28\u91cf\u8981\u6c42\u8f83\u9ad8\uff0c\u9700\u8981\u5bf9\u6837\u672c\u53ca\u6807\u51c6\u54c1DNA\u7eaf\u5316\uff0c\u4fdd\u8bc1\u6837\u672c\u7684\u5747\u4e00\u5316\u3002\u6837\u672cDNA\u7ecfDNA\u7eaf\u5316\u6db2\u5904\u7406\uff0837 \u2103 \u91d1\u5c5e\u6d7430 min\uff0c90 \u2103 \u91d1\u5c5e\u6d742 min\uff09\u540e\uff0c\u53ef\u6839\u636e\u6837\u54c1\u7684\u8d28\u91cf\u5206\u522b\u91c7\u7528\u78c1\u73e0\u5206\u9009\u6216\u5272\u80f6\u56de\u6536\u7684\u65b9\u6cd5\uff0c\u5177\u4f53\u53c2\u8003\u5982\u4e0b\uff1a<\/p>\n

    \uff081\uff09\u78c1\u73e0\u5206\u9009\u65b9\u6cd5\uff1a\u7ecfDNA\u7eaf\u5316\u6db2\u7eaf\u5316\u540e\uff0c\u5982\u679c\u6837\u672cDNA\u8d28\u91cf\u8f83\u597d(\u53c2\u8003\u4e0b\u56fe\u6cf3\u90534-6\uff09\uff0c\u91c7\u7528\u78c1\u73e0\u5206\u9009\u65b9\u6cd5\u3002\u8be6\u7ec6\u7eaf\u5316DNA\u6b65\u9aa4\u89c1\u7aef\u7c92\u957f\u5ea6 DNA\u7eaf\u5316\u8bd5\u5242\u76d2\u8bf4\u660e\u4e66\u3002<\/p>\n

    \uff082\uff09\u5272\u80f6\u56de\u6536\u65b9\u6cd5\uff1a\u7ecfDNA\u7eaf\u5316\u6db2\u7eaf\u5316\u540e\uff0c\u5982\u679c\u6837\u672cDNA\u8d28\u91cf\u8f83\u5dee(\u53c2\u8003\u4e0b\u56fe\u6cf3\u90531-3\uff09\uff0c\u91c7\u7528\u5272\u80f6\u56de\u6536\u65b9\u6cd5\u3002\u5177\u4f53\u64cd\u4f5c\uff1a\u53d61 \u00b5g\u6837\u672c\u8fdb\u884c0.7-0.8%\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u540e\uff0c\u4f7f\u7528TaKaRa MiniBEST Agarose Gel DNA Extraction Kit Ver.4.0(9762)\u56de\u6536\u7eaf\u5316DNA\u7247\u6bb5\uff0c\u56de\u6536\u6548\u738710%-30%\uff0c\u548c\u57fa\u56e0\u7ec4DNA\u7684\u5b8c\u6574\u7a0b\u5ea6\u6709\u5173\u3002<\/p>\n

    \"\u56fe\u72471\"<\/p>\n

      \n
    1. \u6837\u672c\u6d53\u5ea6\u7684\u6807\u5316<\/li>\n<\/ol>\n

      3.1\u25b3\u25b3Ct\u6cd5<\/p>\n

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      3.2 \u6807\u51c6\u66f2\u7ebf\u6cd5<\/p>\n

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      10 ng\uff09\u3002\u6821\u6b63\u54c1\u7684DNA\u6d53\u5ea6\u6807\u5316\u81f34 ng\/\u00b5L\uff0c\u4e0a\u68375 \u00b5L\uff0c\u7528\u4e8e\u6821\u6b63\u3002\u5177\u4f53\u8ba1\u7b97\u8be6\u89c1\u9644\u5f553\u3002<\/p>\n

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      \u9644\u5f55 2<\/p>\n

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      \u88681 \u6807\u51c6\u54c1\u7aef\u7c92\u901a\u9053Ct\u503c<\/strong><\/p>\n

      <\/p>\n

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      <\/p>\n

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      \u88684 \u6821\u6b63\u54c1\u7684\u6821\u6b63\u7cfb\u6570<\/strong><\/p>\n

      \"\u8868\u683c<\/p>\n

      4.\u6837\u672cT\/S\u4ee5\u53ca\u7edd\u5bf9\u957f\u5ea6\u7684\u8ba1\u7b97<\/p>\n

      \u88685 \u6837\u672c\u7684T\/S\u8ba1\u7b97<\/strong><\/p>\n

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      \u6ce8\uff1a\u672c\u8868\u4e2d\u6821\u6b63\u7cfb\u6570\u6e90\u81ea\u88684\u4e2d\u8ba1\u7b97\u51fa\u7684\u6821\u6b63\u7cfb\u6570,\u672c\u8868\u4e2d\u6807\u51c6\u54c1\u7edd\u5bf9\u957f\u5ea6\u4e3a5430 bp<\/strong><\/p>\n

      \u9644\u5f554<\/p>\n

      \u76f8\u5173\u4ea7\u54c1<\/p>\n

      \"\u8868\u683c<\/p>\n

      \u53c2\u8003\u6587\u732e\uff1a<\/p>\n

      \u30101\u3011Sun G, Cao H, Bai Y, Wang J, Zhou Y, Li K, Xiao JH. A novel multiplex qPCR method for assessing the comparative lengths of telomeres. J Clin Lab Anal. 2021 Sep;35(9):e23929. doi: 10.1002\/jcla.23929. Epub 2021 Aug 4. PMID: 34347924; PMCID: PMC8418462.<\/p>\n","protected":false},"excerpt":{"rendered":"

      \u7aef\u7c92\uff08Telomeres\uff09\uff1a\u662f\u771f\u6838\u751f\u7269\u7ebf\u6027\u67d3\u8272\u4f53\u672b\u7aef\u7684\u975e\u7f16\u7801\u4e32\u8054\u91cd\u590d\u6027(TTAGGG)n\u9635\u5217\uff0c\u7aef\u7c92\u957f\u5ea6\u7684\u53d8\u5316\uff0c\u4e0e\u8870\u8001\u3001\u764c\u75c7\u3001\u6216\u795e\u7ecf\u9000\u884c\u6027\u7b49\u591a\u79cd\u75be\u75c5\u76f8\u5173\u3002qPCR\u6cd5\u662f\u68c0\u6d4b\u7aef\u7c92\u957f\u5ea6\u7684\u7ecf\u5178\u6280\u672f\uff0c\u7528\u65f6\u77ed\uff0c\u64cd\u4f5c\u7b80\u5355\uff0c\u7ed3\u679c\u51c6\u786e\uff0c\u9002\u7528\u4e8e\u6279\u91cf\u6837\u672c\u68c0\u6d4b\u3002<\/p>\n

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