{"id":72,"date":"2025-02-11T14:29:07","date_gmt":"2025-02-11T06:29:07","guid":{"rendered":"http:\/\/www.biowing.com.cn\/?post_type=product&p=72"},"modified":"2026-03-22T12:53:24","modified_gmt":"2026-03-22T04:53:24","slug":"xbjyfwglmdzytjc","status":"publish","type":"product","link":"https:\/\/www.biowing.com.cn\/index.php\/product\/xbjyfwglmdzytjc\/","title":{"rendered":"\u652f\u539f\u4f53\u68c0\u6d4b\u8bd5\u5242\u76d2-\u9ad8\u7075\u654f\u5ea6\u7248"},"content":{"rendered":"
\u3010\u4ea7\u54c1\u89c4\u683c\u3011<\/strong>\r\n\r\n\u00a0 \u00a0 \u00a0<\/strong> BPMPro50\u00a0 50 Reactions\/\u76d2\r\n\r\n\u3010\u4ea7\u54c1\u8bf4\u660e\u3011<\/strong>\r\n\r\n\u00a0 \u00a0 \u00a0<\/strong>\u652f\u539f\u4f53(Mycoplasma)\uff1a\u76ee\u524d\u53d1\u73b0\u7684\u6700\u5c0f\u539f\u6838\u751f\u7269\uff0c\u636e\u7edf\u8ba1\u7ea615~35% \u7684\u7ec6\u80de\u88ab\u652f\u539f\u4f53\u6c61\u67d3\u3002\u652f\u539f\u4f53\u6c61\u67d3\u4f1a\u6539\u53d8\u5bbf\u4e3b\u7ec6\u80de\u7684\u7ed3\u6784\u4e0e\u529f\u80fd\uff0c\u6613\u81f4\u5b9e\u9a8c\u7ed3\u679c\u4e0d\u7a33\u5b9a\uff0c\u987b\u5bf9\u57f9\u517b\u7ec6\u80de\u5b9a\u671f\u8fdb\u884c\u652f\u539f\u4f53\u68c0\u6d4b\u3002\r\n\r\n\u672c\u8bd5\u5242\u76d2\u91c7\u7528\u53cc\u8272\u8367\u5149 qPCR (TaqMan \u63a2\u9488\u6cd5) \u68c0\u6d4b\u652f\u539f\u4f53DNA\u3002\u68c0\u6d4b\u5bf9\u8c61\u4e3a\u7ec6\u80de\u57f9\u517b\u7684\u4e0a\u6e05\u6db2\uff0c\u7ec6\u80de\u6c89\u6dc0\u6216\u5176\u4ed6\u751f\u7269\u5236\u54c1\u3002\u8be5\u8bd5\u5242\u76d2\u5177\u6709\u4ee5\u4e0b\u7279\u70b9\uff1a\r\n\r\n\u00a0\u00a0\u7075\u654f<\/strong>\uff1a\u914d\u5408\u63a8\u8350\u4f7f\u7528\u7684\u6838\u9178\u62bd\u63d0\u65b9\u6848\uff0c\u68c0\u6d4b\u7075\u654f\u5ea6\u8fbe\u523010CFU\/ml\u3002\r\n\r\n\u00a0 \u00a0 \u00a0 \u00a0\u00a0\u53ef\u9760<\/strong>\uff1a\u62bd\u63d0\u52a0\u5165\u5185\u6807\u6838\u9178\uff0c\u5168\u8fc7\u7a0b\u8d28\u91cf\u63a7\u5236\u3002\r\n\r\n\u00a0 \u00a0 \u00a0 \u00a0\u00a0\u7a33\u5b9a<\/strong>\uff1a\u5168\u7a0b\u95ed\u7ba1\u64cd\u4f5c\uff0c\u65e0\u4ea4\u53c9\u6c61\u67d3\u3002\r\n\r\n\u00a0 \u00a0 \u00a0 \u00a0\u00a0\u65b9\u4fbf<\/strong>\uff1a\u64cd\u4f5c\u7b80\u5355\uff0c\u65e0\u9700\u7535\u6cf3\u6b65\u9aa4\u3002\r\n\r\n\u3010\u6709\u6548\u671f\u3011<\/strong>\r\n\r\n\u00a0 \u00a0 \u00a0<\/strong>\u89c4\u5b9a\u50a8\u5b58\u6761\u4ef6\u4e0b6\u4e2a\u6708\u3002\r\n\r\n\u00a0 \u00a0 \u00a0-20 \u2103\u53d6\u51fa\u540e\uff0c\u53ef\u907f\u5149\u5b58\u653e\u4e8e4 \u2103\u7ee7\u7eed\u4f7f\u75281\u5468\u3002\r\n\r\n\u3010\u4ea7\u54c1\u7ec4\u5206\u3011<\/strong>\r\n\r\n\u88681\u00a0\u00a0 \u4ea7\u54c1\u7ec4\u5206<\/strong>\r\n\r\n\r\n\r\n\u6ce8\uff1a<\/strong>\r\n\r\n1\u3001\u9633\u6027\u8d28\u63a7\u542b\u6709\u652f\u539f\u4f53DNA\u548c\u5185\u6807DNA\u3002\r\n\r\n2\u3001\u5185\u90e8\u8d28\u63a7\u542b\u6709\u5185\u6807DNA\u3002\r\n\r\n\u5728\u5b9e\u9a8c\u524d\u8bf7\u5b8c\u6574\u9605\u8bfb\u672c\u8bf4\u660e\u4e66\uff0c\u52a1\u5fc5\u91cd\u89c6\u6ce8\u610f\u4e8b\u9879\u548c\u65e0\u83cc\u64cd\u4f5c\u89c4\u8303\uff01<\/strong>\r\n\r\n\u3010\u64cd\u4f5c\u6b65\u9aa4\u3011<\/strong>\r\n\r\n\u00a01.\u6837\u672c\u5236\u5907<\/strong>\r\n\r\n\u00a0 \u00a0 \u00a0<\/strong>\uff081\uff09\u6837\u672c\u7684\u6807\u51c6\u5236\u5907\u65b9\u6848\uff1a\u8bf7\u914d\u5408\u4f7f\u7528\u9ad8\u6548\u65e0\u5fae\u751f\u7269\u6c61\u67d3\u7684DNA\u62bd\u63d0\u8bd5\u5242\u76d2\u63d0\u53d6\u6837\u672cDNA\u3002\u6574\u4e2a\u8fc7\u7a0b\u9700\u8981\u9075\u5b88\u65e0\u83cc\u64cd\u4f5c\u89c4\u8303\u3002\u672c\u516c\u53f8\u76ee\u524d\u63a8\u8350\u62bd\u63d0\u8bd5\u5242\u76d2\u89c1\u9644\u5f553\u3002\u76f4\u63a5\u53d620 \u00b5L\u5f85\u6d4b\u6837\u672cDNA\uff0c\u4f5c\u4e3a\u6a21\u677f\u8fdb\u884c qPCR \u53cd\u5e94\u3002\r\n\r\n\u00a0 \u00a0 \u00a0 \uff082\uff09\u524d\u5904\u7406\u9634\u6027\u6837\u672c\u51c6\u5907\u65b9\u6848\uff1a\u5c06RNase Free Water\u5f53\u6210\u6837\u672c\uff0c\u52a0\u5165\u5185\u90e8\u8d28\u63a7\uff0c\u8fdb\u884c\u6838\u9178\u62bd\u63d0\u3002<\/pre>\n
    \n
  1. \n
    qPCR \u53cd\u5e94\u6db2\u7684\u51c6\u5907<\/pre>\n<\/li>\n<\/ol>\n
    \u00a0 \u00a0 \u00a0 \u00a0\u00a0\uff081\uff09\u6839\u636e\u6240\u8981\u68c0\u6d4b\u6837\u54c1\u7684\u6570\u91cf\uff0c\u8ba1\u7b97\u6240\u9700\u53cd\u5e94\u5b54\u6570\uff0c\u6bcf\u4e2a\u6837\u672c\u505a3\u4e2a\u91cd\u590d\u5b54\u3002\r\n\r\n\u00a0 \u00a0 \u00a0 \u53cd\u5e94\u5b54\u6570=1\u4e2a\u9633\u6027PCR\u8d28\u63a7+1\u4e2a\u9634\u6027PCR\u8d28\u63a7+1\u4e2a\u524d\u5904\u7406\u9634\u6027\u8d28\u63a7+\u6837\u672c\u6570\u00d7 3\r\n\r\n\u00a0 \u00a0 \u00a0 \u00a0\uff082\uff09\u6839\u636e\u53cd\u5e94\u5b54\u6570\u8ba1\u7b97\u6240\u9700\u7684qPCR Mix \u603b\u91cf\uff08\u9700\u67091\u5b54\u7684\u52a0\u6837\u635f\u5931\u91cf\uff09\uff1a\r\n\r\n\u00a0 \u00a0 \u00a0 Mix =\uff08\u53cd\u5e94\u5b54\u6570+1\uff09\u00d710 \u03bcl\r\n\r\n\u00a0 \u00a0 \u00a0 \u00a0\uff083\uff09\u5404\u8bd5\u5242\u653e\u5ba4\u6e29\u878d\u5316\uff0c\u5e76\u6839\u636e\u4e0b\u8868\u6240\u793a\u51c6\u5907 qPCR Mix\uff1a\r\n\r\n\u88682\u00a0 qPCR Mix\u7684\u914d\u5236<\/strong>\r\n\r\n\r\n\r\n\u00a03. \u52a0\u6837<\/strong>\r\n\r\n\u00a0 \u00a0 \u00a0 \u00a0<\/strong>\uff081\uff09\u9707\u8361\u6df7\u5300 qPCR Mix\uff0c\u4f4e\u901f\u79bb\u5fc3\uff0c\u5c06\u7ba1\u76d6\u6b8b\u7559\u6db2\u4f53\u6536\u96c6\u81f3\u7ba1\u5e95\u3002\r\n\r\n\u00a0 \u00a0 \u00a0 \u00a0\uff082\uff09\u5411\u6bcf\u5b54\u53cd\u5e94\u7ba1\u4e2d\u5206\u88c5 10 \u03bcL qPCR Mix\u3002\r\n\r\n\u00a0 \u00a0 \u00a0 \u00a0\uff083\uff09\u5411\u5df2\u5206\u88c5\u8fc7 qPCR Mix \u7684\u53cd\u5e94\u7ba1\u4e2d\u52a0\u516515 ul\u77f3\u8721\u6cb9\u3002\r\n\r\n\u00a0 \u00a0 \u00a0 \u00a0\uff084\uff09\u5411\u53cd\u5e94\u7ba1\u4e2d\u52a0\u5165\u6837\u54c1\u540e\u77ed\u6682\u79bb\u5fc3\uff0c\u52a0\u6837\u793a\u4f8b\u5982\u4e0b\uff1a\r\n\r\n\u00a0 \u00a0 \u00a0 \u00a0\u8868 3 \u52a0\u6837\u793a\u4f8b<\/strong>\r\n\r\n\r\n\r\n\u3010\u6ce8\u3011\uff1a<\/strong>\u6b64\u65f6\u6bcf\u4e2a\u53cd\u5e94\u5b54\u7684\u4f53\u79ef\u4e3a30\u03bcL\u3002\r\n\r\n\u00a0 \u00a0 \u00a0 \u00a0 qPCR \u9634\u6027\u7684PCR\u6a21\u677f\u4e3a20 ul RNase Free Water\u3002\r\n\r\nPCR \u4eea\u8bbe\u7f6e\u4ee5 ABI 7500 \u4e3a\u4f8b\uff0c\u76f8\u5bf9\u5b9a\u91cf\u6a21\u5f0f\uff0c\u9009\u62e9TaqMan \u63a2\u9488\u6cd5\uff1a<\/strong>\r\n\r\n\r\n\r\n\u6ce8\uff1a1\u3001\u8be5\u7a0b\u5e8f\u4e3a\u4e24\u9636\u6bb5\u6269\u589e\u6cd5\uff0c\u5982\u8367\u5149\u5b9a\u91cfPCR\u4eea\u53ef\u4ee5\u4e24\u9636\u6bb5\u6536\u96c6\u8367\u5149\uff0c\u6309\u7167\u6b63\u5e38Ct\u503c\u5224\u65ad\u7ed3\u679c\uff1b\u5982\u679c\u53ea\u80fd\u6700\u540e\u4e00\u6b65\u6536\u96c6\u8367\u5149\uff0c\u9700\u8981\u5728\u539f\u6570\u636e\u7684\u57fa\u7840\u4e0a\u52a015\u4e2aCt\u503c\u3002<\/strong>\r\n\r\n\u00a0 \u00a0 2\u3001ROX\u8bbe\u7f6e\u662f\u4ee57500\u4e3a\u4f8b\uff0c\u4f46\u4e5f\u5b58\u5728\u4f8b\u5916\uff0c\u4f8b\u5982StepOne\u3002<\/strong>\r\n\r\n\u3010\u9608\u503c\u8bbe\u7f6e\u3011<\/strong>\r\n\r\n\u00a0\u00a0\u00a0\u4ee5ABI 7500\u00a0\u4e3a\u4f8b\uff0c\u6269\u589e\u66f2\u7ebf\u9009\u62e9\u00a0log\u00a0\u6cd5\uff0c\u5982\u679c\u4e0d\u80fd\u4e24\u9636\u6bb5\u6536\u96c6\u8367\u5149\uff0c\u57fa\u7ebf\u8bbe\u7f6e1~2\u4e2a\u5faa\u73af\uff1b\u5982\u679c\u53ef\u4ee5\uff0c\u57fa\u7ebf\u8bbe\u7f6e3-15\u4e2a\u5faa\u73af\u3002\u5efa\u8bae\u7528\u00a0log\u00a0\u6cd5\u6269\u589e\u66f2\u7ebf\u4fdd\u8bc1\u7ed3\u679c\u7a33\u5b9a\u53ef\u9760\u3002\r\n\r\n\uff081\uff09 \u5185\u53c2(HEX\/VIC)\u9608\u503c\u8bbe\u5b9a\uff1a\u4ee5\u9633\u6027\u5bf9\u7167\u5b9a\u5185\u53c2\u6269\u589e\u66f2\u7ebf\u9608\u503c\uff0c\u5c06\u5185\u53c2\u7684Ct\u00a0\u503c\u5b9a\u4e3a\u00a028\u00b12\u3002\r\n\r\n\u00a0\u00a0<\/strong>\uff082\uff09\u652f\u539f\u4f53(FAM)\u9608\u503c\u8bbe\u5b9a\uff1a\u4ee5\u9633\u6027\u5bf9\u7167\u5b9a\u652f\u539f\u4f53\u6269\u589e\u66f2\u7ebf\u9608\u503c\uff0c\u5c06\u652f\u539f\u4f53(FAM)\u7684\u00a0Ct\u00a0\u503c\u5b9a\u4e3a\u00a028\u00b12 \u3002\r\n\r\n\u3010\u5b9e\u9a8c\u8d28\u91cf\u63a7\u5236<\/strong>\u3011\r\n\r\n\u00a0\u00a0\u5982\u679c\u9633\u6027\u5bf9\u7167\u7ba1\u7684\u652f\u539f\u4f53(FAM)Ct \u503c>30\uff0c\u4f46\u9633\u6027\u5bf9\u7167\u7ba1\u53ca\u524d\u5904\u7406\u9634\u6027\u5bf9\u7167\u7ba1\u7684\u5185\u53c2(HEX)Ct \u503c\u6b63\u5e38\uff0c\u8868\u660e\u9633\u6027\u652f\u539f\u4f53\u5bf9\u7167\u6a21\u677f\u6709\u964d\u89e3\u3002\r\n\r\n\u5982\u679c\u6837\u54c1\u524d\u5904\u7406\u9634\u6027\u7684\u5185\u53c2\uff08HEX\/VIC)Ct \u503c>30\uff0c\u9633\u6027\u5bf9\u7167\u7ba1\u7684\u5185\u53c2(HEX)Ct \u503c>30 \uff0c\u8868\u660e\u5185\u53c2DNA\u5b58\u5728\u964d\u89e3\u6216PCR\u4f53\u7cfb\u6269\u589e\u6548\u7387\u4e0b\u964d\u3002\r\n\r\n\u00a0 \u00a0 \u3010\u7ed3\u679c\u5224\u8bfb\u3011\r\n\r\n\u00a0\u00a0\u6839\u636e\u652f\u539f\u4f53(FAM)\u00a0Ct \u503c\u3001\u5185\u6807(VIC)\u00a0Ct \u503c\u5224\u5b9a\uff0c\u5177\u4f53\u6807\u51c6\u5982\u4e0b\uff1a\r\n\r\n\r\n\r\n\u8981\u6c42\u6bcf\u4e2a\u68c0\u6d4b\u6837\u54c1\u505a<\/strong>3<\/strong>\u91cd\u590d\uff0c\u5982\u679c<\/strong>3<\/strong>\u590d\u5b54\u4e2d\uff0c\u4e24\u91cd\u590d\u4e3a\u9633\u6027\uff0c\u5219\u5224\u8bfb\u4e3a\u9633\u6027\uff1b\u5efa\u8bae\u505a\u6837\u672c\u524d\u5904\u7406\u9634\u6027\uff0c\u53ef\u4ee5\u8d28\u63a7\u6574\u4e2a\u62bd\u63d0\u8fc7\u7a0b\u3002<\/strong>\r\n\r\n\u00a0<\/strong>\r\n\r\n\u6ce8\uff1a<\/strong>\u68c0\u6d4b\u7ed3\u679c\u5f02\u5e38\u65f6\uff1a<\/pre>\n
      \n
    1. \n
      \u6837\u54c1\u524d\u5904\u7406\u9634\u6027\uff1a\u5185\u53c2Ct\u503c\u8fc7\u5927\uff0c\u8868\u660e\u62bd\u63d0\u6548\u7387\u4f4e\uff1b\u652f\u539f\u4f53\u68c0\u6d4b\u901a\u9053Ct\u503c\u5c0f\u4e8e37.5\uff0c\u53ef\u80fd\u524d\u5904\u7406\u62bd\u63d0\u8fc7\u7a0b\u5b58\u5728\u6c61\u67d3\u3002<\/pre>\n<\/li>\n
    2. \n
      qPCR\u9634\u6027\uff1a\u652f\u539f\u4f53\u548c\u5185\u53c2\u68c0\u6d4b\u901a\u9053Ct\u503c\u5c0f\u4e8e37.5\uff0c\u64cd\u4f5c\u8fc7\u7a0b\u5b58\u5728\u6c61\u67d3\u3002<\/pre>\n<\/li>\n
    3. \n
      \u6837\u672c\u524d\u5904\u7406\u9634\u6027\u8d28\u63a7\u548cPCR\u9633\u6027\u8d28\u63a7\u5185\u53c2\u5dee1\u00b11\u4e2aCt\u503c\u5c5e\u4e8e\u6b63\u5e38\u73b0\u8c61\u3002<\/pre>\n<\/li>\n<\/ol>\n
      \u3010PCR\u8fc7\u7a0b\u6ce8\u610f\u4e8b\u9879\u3011\r\n\r\n\u7531\u4e8e\u00a0PCR \u53cd\u5e94\u975e\u5e38\u7075\u654f\uff0c\u5b9e\u9a8c\u64cd\u4f5c\u4e2d\u5e94\u6ce8\u610f\u4ee5\u4e0b\u4e8b\u9879\uff0c\u4ee5\u514d\u53d1\u751fDNA \u6c61\u67d3\uff1a\r\n\r\n\u00a0 \u00a01\uff0e\u8bd5\u5242\u76d2\u5f00\u542f\u540e\uff0c\u9633\u6027\u8d28\u63a7\u548c\u5185\u90e8\u8d28\u63a7\u4e0e\u8bd5\u5242Biowing\u00ae MyqPCR Reaction Mix1\u3001Biowing\u00aeMyPrimer&Probe Mix2\u3001RNase Free Water\u3001\u77f3\u8721\u6cb9\u5206\u5f00\u5b58\u653e\u3002\r\n\r\n\u00a0 \u00a0 2\uff0e\u6bcf\u4e2a\u7ec4\u5206\u5728\u4f7f\u7528\u524d\u90fd\u5e94\u5148\u4f4e\u901f\u79bb\u5fc3\u540e\u5c0f\u5fc3\u5f00\u542f\u3002\u4f7f\u7528\u65f6\u8bf7\u4e0a\u4e0b\u8f7b\u67d4\u98a0\u5012\u5341\u6b21\u6df7\u5300\uff0c\u907f\u514d\u8d77\u6ce1\uff0c\u5e76\u4f4e\u901f\u77ed\u6682\u79bb\u5fc3\u540e\u4f7f\u7528\u3002\r\n\r\n\u00a0 \u00a03\uff0e\u8981\u6c42\u6838\u9178\u62bd\u63d0\u548cqPCR\u8fc7\u7a0b\u90fd\u7b26\u5408\u65e0\u83cc\u64cd\u4f5c\u89c4\u8303\u3002\r\n\r\n\u00a0 \u00a04\uff0e\u5efa\u8bae\u81ea\u5907\u8f6c\u8fd0\u76d2\uff0c\u7528\u4e8e\u5c06\u914d\u5236\u5206\u88c5\u597d\u7684Mix\u4ece\u914d\u7f6eMix\u8d85\u51c0\u5de5\u4f5c\u53f0\u8f6c\u8fd0\u5230\u52a0\u6837\u8d85\u51c0\u5de5\u4f5c\u53f0\u3002\r\n\r\n\u00a0 \u00a05\uff0e\u5efa\u8bae\u5728 qPCR \u53cd\u5e94\u677f\u4e0a\u9633\u6027\u5bf9\u7167\u7684\u6392\u5e03\u5e94\u8fdc\u79bb\u5f85\u6d4b\u6837\u672c\u548c\u9634\u6027\u5bf9\u7167\u3002\r\n\r\n\u00a0 \u00a06\uff0e\u5efa\u8bae qPCR \u914d\u7f6e\u4e0e\u5206\u88c5\u5728\u4e00\u4e2a\u65e0\u83cc\u64cd\u4f5c\u53f0\uff0c\u6837\u672c\u7684\u52a0\u6837\u5728\u53e6\u5916\u4e00\u4e2a\u65e0\u83cc\u64cd\u4f5c\u53f0\u3002\r\n\r\n\u00a0 \u00a07\uff0e\u5efa\u8bae\u4f7f\u7528\u4e0d\u540c\u7684\u79fb\u6db2\u5668\u6dfb\u52a0\u9633\u6027\u5bf9\u7167\u4e0e\u5f85\u6d4b\u6837\u672c\u3001\u9634\u6027\u5bf9\u7167\uff0c\u5e76\u4f7f\u7528\u5e26\u6ee4\u82af\u7684\u67aa\u5934\u8fdb\u884c\u52a0\u6837\u3002\r\n\r\n\u00a0 \u00a08\uff0e\u5e94\u6309\u7167\u5148\u52a0\u9634\u6027\uff0c\u518d\u52a0\u6837\u672c\uff0c\u6700\u540e\u52a0\u9633\u6027\u7684\u987a\u5e8f\u52a0\u6837\u3002\u4e14\u9633\u6027\u7528\u4e13\u95e8\u7684\u52a0\u6837\u5668\u3002\u5efa\u8bae\u9633\u6027\u5728\u4e13\u95e8\u7684\u65e0\u83cc\u64cd\u4f5c\u53f0\u52a0\u6837\u3002\r\n\r\n\u00a0 \u00a09\uff0eqPCR \u53cd\u5e94\u677f\u5c01\u819c\u6216\u76d6\u76d6\u5b50\u65f6\u987b\u53cd\u590d\u538b\u7d27\u3002\r\n\r\n\u00a0 10\uff0e\u4e0a\u673a\u6269\u589e\u524d\uff0c\u53cd\u5e94\u7ba1\u77ed\u65f6\u4f4e\u901f\u79bb\u5fc3\uff0c\u5c06\u7ba1\u58c1\u6db2\u4f53\u6536\u96c6\u81f3\u7ba1\u5e95\r\n\r\n\u00a0 11\uff0e\u76d6\u4e0a\u53cd\u5e94\u7ba1\u76d6\u5b50\u6216\u8005\u8d34\u4e0a\u5149\u5b66\u819c\u540e\uff0c\u4e3a\u907f\u514d\u5f71\u54cd\u8367\u5149\u4fe1\u53f7\u8bfb\u53d6\uff0c\u8bf7\u6ce8\u610f\u4e0d\u8981\u5728\u7ba1\u76d6\u6216\u8005\u819c\u4e0a\u505a\u6807\u8bb0\uff0c\u6216\u8005\u7528\u522e\u677f\u53cd\u590d\u6469\u64e6\u3002\r\n\r\n\u00a0 12\uff0e\u672c\u4ea7\u54c1\u4ec5\u4f5c\u79d1\u7814\u7528\u9014\u3002<\/pre>\n","protected":false},"excerpt":{"rendered":"
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