{"id":922,"date":"2026-06-01T13:11:48","date_gmt":"2026-06-01T05:11:48","guid":{"rendered":"https:\/\/www.biowing.com.cn\/?post_type=product&p=922"},"modified":"2026-06-01T13:11:48","modified_gmt":"2026-06-01T05:11:48","slug":"szpcrjcszpcrdptbyxltdnakbsbyjc","status":"publish","type":"product","link":"https:\/\/www.biowing.com.cn\/index.php\/product\/szpcrjcszpcrdptbyxltdnakbsbyjc\/","title":{"rendered":"\u6570\u5b57PCR\u4f4e\u9891\u7a81\u53d8\u4e0e\u7ebf\u7c92\u4f53DNA\u62f7\u8d1d\u6570\u53d8\u5f02\u68c0\u6d4b"},"content":{"rendered":"

\u6570\u5b57PCR\u4f4e\u9891\u7a81\u53d8\u4e0e\u7ebf\u7c92\u4f53DNA\u62f7\u8d1d\u6570\u53d8\u5f02\u68c0\u6d4b<\/strong><\/p>\n

\u4e00\u3001\u6570\u5b57PCR\u4f4e\u9891\u7a81\u53d8\u68c0\u6d4b<\/strong><\/p>\n

\u3010\u9879\u76ee\u4ecb\u7ecd\u3011<\/strong><\/p>\n

\u4f4e\u9891\u7a81\u53d8\u662f\u6307\u7a81\u53d8\u7b49\u4f4d\u57fa\u56e0\u5728\u5927\u91cf\u91ce\u751f\u578b\u80cc\u666f\u4e2d\u6240\u5360\u6bd4\u4f8b\u8f83\u4f4e\u7684\u9057\u4f20\u53d8\u5f02\uff0c\u5e38\u89c1\u4e8e\u80bf\u7624\u6db2\u4f53\u6d3b\u68c0\u3001\u8010\u836f\u7a81\u53d8\u76d1\u6d4b\u3001FFPE\u5fae\u91cf\u6837\u672c\u3001\u7ec6\u80de\u7cfb\u6df7\u5408\u6837\u672c\u3001\u57fa\u56e0\u7f16\u8f91\u4f4e\u6bd4\u4f8b\u7a81\u53d8\u4ee5\u53ca\u6a21\u578b\u6784\u5efa\u8fc7\u7a0b\u4e2d\u7684\u4e9a\u514b\u9686\u7b5b\u67e5\u3002\u4f20\u7edfSanger\u6d4b\u5e8f\u548c\u5e38\u89c4qPCR\u5728\u4f4e\u7a81\u53d8\u4e30\u5ea6\u80cc\u666f\u4e0b\u5bb9\u6613\u53d7\u5230\u80cc\u666f\u566a\u58f0\u3001\u6269\u589e\u504f\u501a\u548c\u5b9a\u91cf\u6807\u51c6\u66f2\u7ebf\u7684\u5f71\u54cd\uff0c\u800c\u9ad8\u6df1\u5ea6\u6d4b\u5e8f\u867d\u53ef\u5b9e\u73b0\u591a\u4f4d\u70b9\u5206\u6790\uff0c\u4f46\u5728\u5355\u4e2a\u4f4d\u70b9\u5feb\u901f\u9a8c\u8bc1\u3001\u4f4e\u9891\u9633\u6027\u590d\u6838\u548c\u7edd\u5bf9\u5b9a\u91cf\u65b9\u9762\u4ecd\u9700\u7ed3\u5408\u66f4\u76f4\u63a5\u7684\u68c0\u6d4b\u624b\u6bb5\u3002<\/p>\n

\u672c\u9879\u76ee\u91c7\u7528\u6570\u5b57PCR\u6280\u672f\u5bf9\u5df2\u77e5SNV\u6216\u5c0f\u7247\u6bb5\u63d2\u5165\u7f3a\u5931\u7a81\u53d8\u8fdb\u884c\u5b9a\u91cf\u68c0\u6d4b\u3002\u68c0\u6d4b\u4f53\u7cfb\u5c06\u53cd\u5e94\u6db2\u5206\u914d\u81f3\u5927\u91cf\u72ec\u7acb\u5fae\u53cd\u5e94\u5355\u5143\u4e2d\uff0c\u4f7f\u7a81\u53d8\u6a21\u677f\u548c\u91ce\u751f\u578b\u6a21\u677f\u5728\u5206\u533a\u5185\u72ec\u7acb\u6269\u589e\uff0c\u5e76\u901a\u8fc7\u7a81\u53d8\u578b\u63a2\u9488\u4e0e\u91ce\u751f\u578b\u63a2\u9488\u7684\u53cc\u901a\u9053\u8367\u5149\u4fe1\u53f7\u5b8c\u6210\u5206\u7fa4\u5224\u8bfb\u3002\u7cfb\u7edf\u6839\u636e\u9633\u6027\u5206\u533a\u6570\u8fdb\u884cPoisson\u6821\u6b63\uff0c\u76f4\u63a5\u8f93\u51fa\u7a81\u53d8\u578b\u62f7\u8d1d\u6570\u3001\u91ce\u751f\u578b\u62f7\u8d1d\u6570\u548c\u7a81\u53d8\u7b49\u4f4d\u57fa\u56e0\u9891\u7387\uff08variant allele frequency, VAF\uff09\u3002\u4e0e\u76f8\u5bf9\u5b9a\u91cf\u65b9\u6cd5\u76f8\u6bd4\uff0c\u6570\u5b57PCR\u4e0d\u4f9d\u8d56\u6807\u51c6\u66f2\u7ebf\uff0c\u9002\u5408\u4f4e\u4e30\u5ea6\u7a81\u53d8\u7684\u7edd\u5bf9\u5b9a\u91cf\u3001\u52a8\u6001\u76d1\u6d4b\u548c\u9634\u9633\u6027\u8fb9\u754c\u6837\u672c\u590d\u6838\u3002<\/p>\n

\u4f4e\u9891\u7a81\u53d8\u68c0\u6d4b\u7684\u5173\u952e\u4e0d\u53ea\u662f\u201c\u80fd\u5426\u68c0\u51fa\u201d\uff0c\u8fd8\u5305\u62ec\u9634\u6027\u80cc\u666f\u4e0b\u5047\u9633\u6027\u6ef4\u5ea6\u3001\u6700\u4f4e\u7a7a\u767d\u9650\uff08LoB\uff09\u3001\u6700\u4f4e\u68c0\u51fa\u9650\uff08LoD\uff09\u3001\u6700\u4f4e\u5b9a\u91cf\u9650\u3001\u8f93\u5165DNA\u91cf\u4ee5\u53ca\u7a81\u53d8\u9633\u6027\u5206\u533a\u7684\u91cd\u590d\u6027\u3002\u56e0\u6b64\uff0c\u672c\u9879\u76ee\u5728\u68c0\u6d4b\u524d\u53ef\u6839\u636e\u76ee\u6807\u4f4d\u70b9\u5efa\u7acb\u91ce\u751f\u578b\u9634\u6027\u80cc\u666f\u3001\u9633\u6027\u68af\u5ea6\u6807\u51c6\u54c1\u548c\u7a7a\u767d\u5bf9\u7167\uff0c\u5e76\u7ed3\u5408\u5206\u533a\u6570\u3001\u8367\u5149\u5206\u7fa4\u8d28\u91cf\u548c\u91cd\u590d\u5b54\u4e00\u81f4\u6027\u8fdb\u884c\u7efc\u5408\u5224\u5b9a\uff0c\u907f\u514d\u5355\u7eaf\u4f9d\u636e\u5e73\u53f0\u7406\u8bba\u7075\u654f\u5ea6\u8fdb\u884c\u8fc7\u5ea6\u89e3\u91ca\u3002<\/p>\n

<\/p>\n

\u56fe1 \u4f4e\u9891\u7a81\u53d8\u6570\u5b57PCR\u68c0\u6d4b\u539f\u7406\u793a\u610f\u56fe<\/p>\n

\u3010\u68c0\u6d4b\u5bf9\u8c61\u3011<\/strong><\/p>\n

\u2022 \u5df2\u77e5\u4f4e\u9891SNV\u6216\u5c0f\u7247\u6bb5\u63d2\u5165\u7f3a\u5931\u7a81\u53d8\uff0c\u5982EGFR\u3001KRAS\u3001BRAF\u3001TP53\u3001PIK3CA\u7b49\u70ed\u70b9\u4f4d\u70b9\uff0c\u4e5f\u53ef\u6839\u636e\u5ba2\u6237\u63d0\u4f9b\u7684\u57fa\u56e0\u5750\u6807\u8fdb\u884c\u5b9a\u5236\u5f00\u53d1\u3002<\/p>\n

\u2022 \u8840\u6d46\/\u8840\u6e05\u6765\u6e90cfDNA\u3001FFPE\u7ec4\u7ec7DNA\u3001\u65b0\u9c9c\u6216\u51bb\u5b58\u7ec4\u7ec7DNA\u3001\u57f9\u517b\u7ec6\u80de\u57fa\u56e0\u7ec4DNA\u3001\u7ec6\u80de\u7cfb\u6df7\u5408\u6837\u672c\u53ca\u57fa\u56e0\u7f16\u8f91\u6837\u672c\u3002<\/p>\n

\u2022 \u9002\u7528\u4e8e\u4eba\u6e90\u6837\u672c\u53ca\u5e38\u89c1\u6a21\u5f0f\u52a8\u7269\u6837\u672c\uff0c\u9700\u660e\u786e\u7269\u79cd\u3001\u53c2\u8003\u57fa\u56e0\u7ec4\u7248\u672c\u3001\u76ee\u6807\u5750\u6807\u3001\u53c2\u8003\u78b1\u57fa\u548c\u7a81\u53d8\u78b1\u57fa\u3002<\/p>\n

\u3010\u9002\u7528\u8303\u56f4\u3011<\/strong><\/p>\n\n\n\n\n\n\n\n
\u5e94\u7528\u65b9\u5411<\/strong><\/td>\n\u8bf4\u660e<\/strong><\/td>\n<\/tr>\n
\u4f4eVAF\u7a81\u53d8\u5b9a\u91cf<\/td>\n\u5bf9\u5df2\u77e5\u7a81\u53d8\u4f4d\u70b9\u8fdb\u884c\u7edd\u5bf9\u62f7\u8d1d\u6570\u548cVAF\u8ba1\u7b97\uff0c\u9002\u5408\u4f4e\u4e30\u5ea6\u9633\u6027\u6837\u672c\u590d\u6838\u3002<\/td>\n<\/tr>\n
\u6db2\u4f53\u6d3b\u68c0\u4e0e\u8010\u836f\u76d1\u6d4b\u7814\u7a76<\/td>\n\u7528\u4e8e\u8840\u6d46cfDNA\u4e2dEGFR T790M\u3001KRAS\u7b49\u8010\u836f\u6216\u9a71\u52a8\u7a81\u53d8\u7684\u7814\u7a76\u6027\u68c0\u6d4b\u3002<\/td>\n<\/tr>\n
NGS\/qPCR\u7ed3\u679c\u9a8c\u8bc1<\/td>\n\u5bf9\u6d4b\u5e8f\u53d1\u73b0\u7684\u4f4e\u9891\u5019\u9009\u7a81\u53d8\u8fdb\u884c\u5355\u4e2a\u4f4d\u70b9\u5feb\u901f\u9a8c\u8bc1\uff0c\u8f85\u52a9\u6392\u9664\u6d4b\u5e8f\u566a\u58f0\u3002<\/td>\n<\/tr>\n
\u7ec6\u80de\u6a21\u578b\/\u6df7\u6837\u8bc4\u4f30<\/td>\n\u7528\u4e8e\u7ec6\u80de\u7cfb\u4f4e\u6bd4\u4f8b\u7a81\u53d8\u3001\u4e9a\u514b\u9686\u7b5b\u67e5\u3001\u6df7\u6837\u6c61\u67d3\u6216\u57fa\u56e0\u7f16\u8f91\u7a81\u53d8\u6bd4\u4f8b\u8bc4\u4f30\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u3010\u5408\u4f5c\u65b9\u5f0f\u4e0e\u6837\u672c\u8981\u6c42\u3011<\/strong><\/p>\n

\u2022 \u5ba2\u6237\u63d0\u4f9b\u76ee\u6807\u7a81\u53d8\u4f4d\u70b9\u4fe1\u606f\uff0c\u5305\u62ec\u57fa\u56e0\u540d\u79f0\u3001\u53c2\u8003\u57fa\u56e0\u7ec4\u7248\u672c\u3001\u67d3\u8272\u4f53\u5750\u6807\u3001\u53c2\u8003\/\u7a81\u53d8\u78b1\u57fa\u6216\u7a81\u53d8\u5e8f\u5217\uff0c\u6700\u597d\u540c\u65f6\u63d0\u4f9b\u4e0a\u4e0b\u6e38\u7ea6200 bp\u5e8f\u5217\u3002<\/p>\n

\u2022 \u6837\u672c\u53ef\u63d0\u4f9b\u63d0\u53d6\u597d\u7684DNA\u6216\u539f\u59cb\u6837\u672c\u3002\u57fa\u56e0\u7ec4DNA\u5efa\u8bae\u603b\u91cf\u226550 ng\uff1bcfDNA\u548cFFPE DNA\u53ef\u6839\u636e\u6837\u672c\u91cf\u5c3d\u91cf\u63d0\u4f9b\uff0c\u4f4e\u8f93\u5165\u6837\u672c\u9700\u589e\u52a0\u91cd\u590d\u5b54\u5e76\u964d\u4f4e\u5b9a\u91cf\u627f\u8bfa\u3002<\/p>\n

\u2022 \u5efa\u8bae\u540c\u6b65\u63d0\u4f9b\u91ce\u751f\u578b\u9634\u6027\u6837\u672c\u3001\u7a81\u53d8\u9633\u6027\u6807\u51c6\u54c1\u6216\u5df2\u77e5\u9633\u6027\u6837\u672c\uff0c\u7528\u4e8e\u9608\u503c\u8bbe\u5b9a\u3001\u80cc\u666f\u8bc4\u4f30\u548cLoD\u9a8c\u8bc1\u3002<\/p>\n

\u2022 DNA\u6837\u672c\u5efa\u8bae4 \u00b0C\u77ed\u9014\u8fd0\u8f93\u6216\u221220 \u00b0C\u4fdd\u5b58\u8fd0\u8f93\uff1b\u8840\u6d46\u548c\u7ec4\u7ec7\u6837\u672c\u6309\u5e38\u89c4\u6838\u9178\u68c0\u6d4b\u6837\u672c\u8fd0\u8f93\u8981\u6c42\u6267\u884c\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002<\/p>\n

\u3010\u7ed3\u679c\u8f93\u51fa\u3011<\/strong><\/p>\n

\u62a5\u544a\u53ef\u8f93\u51fa\u7a81\u53d8\u578b\u62f7\u8d1d\u6570\u3001\u91ce\u751f\u578b\u62f7\u8d1d\u6570\u3001\u603b\u76ee\u6807\u62f7\u8d1d\u6570\u3001VAF\u3001\u9633\u6027\/\u9634\u6027\u5224\u8bfb\u3001\u91cd\u590d\u5b54CV\u3001\u4e8c\u7ef4\u8367\u5149\u5206\u7fa4\u56fe\u53ca\u8d28\u63a7\u8bf4\u660e\u3002\u82e5\u7528\u4e8e\u6b63\u5f0f\u65b9\u6cd5\u5b66\u9a8c\u8bc1\uff0c\u53ef\u8fdb\u4e00\u6b65\u7ed9\u51fa\u7ebf\u6027\u8303\u56f4\u3001LoB\u3001LoD\u3001\u91cd\u590d\u6027\u548c\u6279\u95f4\u4e00\u81f4\u6027\u7ed3\u679c\u3002<\/p>\n

\u3010\u7ed3\u679c\u5c55\u793a\u5f62\u5f0f\u793a\u4f8b\u3011<\/strong><\/p>\n\n\n\n\n\n\n
\u6837\u672c\u7c7b\u578b<\/strong><\/td>\n\u8f93\u51fa\u6307\u6807<\/strong><\/td>\n\u7ed3\u679c\u89e3\u91ca<\/strong><\/td>\n<\/tr>\n
\u91ce\u751f\u578b\u9634\u6027\u80cc\u666f<\/td>\n\u7a81\u53d8\u901a\u9053\u80cc\u666f\u9633\u6027\u5206\u533a\u6570\u3001WT\u62f7\u8d1d\u6570<\/td>\n\u7528\u4e8e\u786e\u5b9aLoB\u548c\u9608\u503c\uff0c\u8bc4\u4f30\u5047\u9633\u6027\u80cc\u666f\u3002<\/td>\n<\/tr>\n
\u4f4e\u9891\u9633\u6027\u6807\u51c6\u54c1<\/td>\nMut\u62f7\u8d1d\u6570\u3001WT\u62f7\u8d1d\u6570\u3001\u7406\u8bbaVAF\u4e0e\u5b9e\u6d4bVAF<\/td>\n\u7528\u4e8e\u9a8c\u8bc1\u4e0d\u540c\u7a81\u53d8\u4e30\u5ea6\u4e0b\u7684\u68c0\u51fa\u80fd\u529b\u548c\u5b9a\u91cf\u7ebf\u6027\u3002<\/td>\n<\/tr>\n
\u5f85\u6d4b<\/td>\nMut\u62f7\u8d1d\u6570\u3001WT\u62f7\u8d1d\u6570\u3001VAF\u3001\u7f6e\u4fe1\u533a\u95f4<\/td>\n\u7ed3\u5408LoB\/LoD\u3001\u91cd\u590d\u5b54\u4e00\u81f4\u6027\u548c\u5206\u7fa4\u8d28\u91cf\u7ed9\u51fa\u5224\u8bfb\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4e8c\u3001\u6570\u5b57PCR\u7ebf\u7c92\u4f53DNA\u62f7\u8d1d\u6570\u53d8\u5f02\u68c0\u6d4b<\/strong><\/p>\n

\u3010\u9879\u76ee\u4ecb\u7ecd\u3011<\/strong><\/p>\n

\u7ebf\u7c92\u4f53DNA\u62f7\u8d1d\u6570\uff08mitochondrial DNA copy number, mtDNA-CN\uff09\u53cd\u6620\u7ec6\u80de\u5185\u7ebf\u7c92\u4f53\u57fa\u56e0\u7ec4\u7684\u76f8\u5bf9\u542b\u91cf\uff0c\u662f\u8bc4\u4ef7\u7ebf\u7c92\u4f53\u751f\u7269\u53d1\u751f\u3001\u80fd\u91cf\u4ee3\u8c22\u72b6\u6001\u3001\u6c27\u5316\u5e94\u6fc0\u53cd\u5e94\u3001\u836f\u7269\u6bd2\u6027\u548c\u75be\u75c5\u76f8\u5173\u7ebf\u7c92\u4f53\u529f\u80fd\u6539\u53d8\u7684\u91cd\u8981\u6307\u6807\u3002\u4e0d\u540c\u7ec4\u7ec7\u3001\u7ec6\u80de\u7c7b\u578b\u3001\u5904\u7406\u6761\u4ef6\u548c\u75be\u75c5\u72b6\u6001\u4e0b\uff0cmtDNA\u62f7\u8d1d\u6570\u53ef\u80fd\u51fa\u73b0\u5347\u9ad8\u6216\u964d\u4f4e\uff0c\u56e0\u6b64\u5e38\u88ab\u7528\u4e8e\u7ec6\u80de\u529f\u80fd\u7814\u7a76\u3001\u80bf\u7624\u4e0e\u4ee3\u8c22\u75be\u75c5\u7814\u7a76\u3001\u8870\u8001\u76f8\u5173\u7814\u7a76\u4ee5\u53ca\u7ec6\u80de\u6a21\u578b\u8d28\u91cf\u8bc4\u4ef7\u3002<\/p>\n

\u4f20\u7edfqPCR\u901a\u5e38\u901a\u8fc7mtDNA\u9776\u70b9\u4e0e\u6838\u57fa\u56e0\u5185\u53c2\u7684\u0394Ct\u6216\u76f8\u5bf9\u6bd4\u503c\u63a8\u7b97mtDNA\u542b\u91cf\uff0c\u4f46\u5176\u7ed3\u679c\u5bb9\u6613\u53d7\u5230\u6269\u589e\u6548\u7387\u3001\u6807\u51c6\u66f2\u7ebf\u3001Ct\u9608\u503c\u548c\u6279\u95f4\u5dee\u5f02\u5f71\u54cd\u3002\u6570\u5b57PCR\u901a\u8fc7\u5927\u91cf\u5206\u533a\u7ec8\u70b9\u6269\u589e\u5b9e\u73b0\u7edd\u5bf9\u5b9a\u91cf\uff0c\u53ef\u540c\u65f6\u68c0\u6d4bmtDNA\u9776\u70b9\u548c\u6838\u57fa\u56e0\u5355\u62f7\u8d1d\u5185\u53c2\uff0c\u5e76\u76f4\u63a5\u6839\u636e\u9633\u6027\u5206\u533a\u6570\u8ba1\u7b97\u4e24\u7c7b\u9776\u6807\u7684\u62f7\u8d1d\u6570\u3002\u5bf9\u4e8e\u4e8c\u500d\u4f53\u7ec6\u80de\u6837\u672c\uff0c\u53ef\u6309\u201cmtDNA\u62f7\u8d1d\u6570\/\u7ec6\u80de = 2 \u00d7 mtDNA\u9776\u70b9\u62f7\u8d1d\u6570 \/ \u6838\u5355\u62f7\u8d1d\u57fa\u56e0\u62f7\u8d1d\u6570\u201d\u8ba1\u7b97\uff0c\u4e5f\u53ef\u8f93\u51famtDNA\/nDNA\u76f8\u5bf9\u6bd4\u503c\u7528\u4e8e\u4e0d\u540c\u5904\u7406\u7ec4\u6216\u4e0d\u540c\u6837\u672c\u95f4\u6bd4\u8f83\u3002<\/p>\n

\u672c\u9879\u76ee\u53ef\u6839\u636e\u7269\u79cd\u548c\u6837\u672c\u7c7b\u578b\u8bbe\u8ba1mtDNA\u9776\u70b9\u4e0e\u6838\u57fa\u56e0\u5185\u53c2\u68c0\u6d4b\u4f53\u7cfb\u3002\u9776\u70b9\u9009\u62e9\u65f6\u4f18\u5148\u8003\u8651ND1\u3001ND4\u3001CYTB\u7b49\u7ebf\u7c92\u4f53\u7f16\u7801\u533a\uff0c\u5e76\u5bf9\u6838\u57fa\u56e0\u7ec4\u4e2d\u7684\u7ebf\u7c92\u4f53\u540c\u6e90\u7247\u6bb5\uff08NUMTs\uff09\u8fdb\u884c\u5e8f\u5217\u6392\u67e5\u3002\u7531\u4e8eD-loop\u533a\u57df\u53ef\u80fd\u53d7\u5230\u590d\u5236\u4e2d\u95f4\u4f53\u30017S DNA\u6216\u533a\u57df\u7279\u5f02\u6027\u6269\u589e\u504f\u5dee\u5f71\u54cd\uff0c\u4e0d\u5efa\u8bae\u5c06\u5176\u4f5c\u4e3a\u552f\u4e00\u7684mtDNA\u62f7\u8d1d\u6570\u5b9a\u91cf\u9776\u70b9\u3002\u5bf9\u4e8e\u8840\u6db2\u3001\u7ec4\u7ec7\u6216\u7ec6\u80de\u6837\u672c\uff0c\u9700\u4fdd\u6301\u6837\u672c\u5904\u7406\u548cDNA\u63d0\u53d6\u6d41\u7a0b\u4e00\u81f4\uff0c\u4ee5\u51cf\u5c11\u4e0d\u540c\u7c7b\u578bDNA\u56de\u6536\u6548\u7387\u5dee\u5f02\u5e26\u6765\u7684\u7cfb\u7edf\u504f\u501a\u3002<\/p>\n

<\/p>\n

\u56fe2 \u7ebf\u7c92\u4f53DNA\u62f7\u8d1d\u6570\u53d8\u5f02\u6570\u5b57PCR\u68c0\u6d4b\u539f\u7406\u793a\u610f\u56fe<\/p>\n

\u3010\u68c0\u6d4b\u5bf9\u8c61\u3011<\/strong><\/p>\n

\u2022 \u57f9\u517b\u7ec6\u80de\u3001\u539f\u4ee3\u7ec6\u80de\u3001\u5e72\u7ec6\u80de\u3001\u7c7b\u5668\u5b98\u3001\u7ec4\u7ec7\u6837\u672c\u3001\u8840\u6db2\u7ec6\u80de\u53ca\u63d0\u53d6\u540e\u7684\u57fa\u56e0\u7ec4DNA\u3002<\/p>\n

\u2022 \u53ef\u7528\u4e8e\u4eba\u3001\u5c0f\u9f20\u3001\u5927\u9f20\u53ca\u5176\u4ed6\u5df2\u77e5\u7ebf\u7c92\u4f53\u57fa\u56e0\u7ec4\u5e8f\u5217\u7684\u6837\u672c\u3002<\/p>\n

\u2022 \u9002\u7528\u4e8e\u836f\u7269\u5904\u7406\u3001\u6c27\u5316\u5e94\u6fc0\u3001\u4ee3\u8c22\u8c03\u63a7\u3001\u75be\u75c5\u6a21\u578b\u3001\u7ec6\u80de\u8870\u8001\u548c\u7ebf\u7c92\u4f53\u529f\u80fd\u76f8\u5173\u7814\u7a76\u6837\u672c\u3002<\/p>\n

\u3010\u9002\u7528\u8303\u56f4\u3011<\/strong><\/p>\n\n\n\n\n\n\n\n
\u5e94\u7528\u65b9\u5411<\/strong><\/td>\n\u8bf4\u660e<\/strong><\/td>\n<\/tr>\n
mtDNA\u7edd\u5bf9\u62f7\u8d1d\u6570\u68c0\u6d4b<\/td>\n\u8f93\u51fa\u6bcf\u7ec6\u80demtDNA\u62f7\u8d1d\u6570\u6216\u5355\u4f4dDNA\u4e2d\u7684mtDNA\/nDNA\u6bd4\u503c\u3002<\/td>\n<\/tr>\n
\u529f\u80fd\u8bc4\u4ef7<\/td>\n\u6bd4\u8f83\u836f\u7269\u3001\u5e94\u6fc0\u3001\u57f9\u517b\u6761\u4ef6\u6216\u57fa\u56e0\u5e72\u9884\u524d\u540emtDNA\u542b\u91cf\u53d8\u5316\u3002<\/td>\n<\/tr>\n
\u75be\u75c5\u548c\u6a21\u578b\u6837\u672c\u6bd4\u8f83<\/td>\n\u7528\u4e8e\u80bf\u7624\u3001\u4ee3\u8c22\u3001\u795e\u7ecf\u9000\u884c\u6027\u3001\u8870\u8001\u7b49\u7814\u7a76\u4e2d\u7684\u7ec4\u95f4\u5dee\u5f02\u5206\u6790\u3002<\/td>\n<\/tr>\n
\u7ec6\u80de\u8d28\u91cf\u63a7\u5236<\/td>\n\u8bc4\u4ef7\u957f\u671f\u4f20\u4ee3\u3001\u51bb\u878d\u3001\u8bf1\u5bfc\u5206\u5316\u6216\u7ec6\u80de\u6a21\u578b\u6784\u5efa\u8fc7\u7a0b\u4e2d\u7684\u7ebf\u7c92\u4f53\u62f7\u8d1d\u6570\u53d8\u5316\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u3010\u5408\u4f5c\u65b9\u5f0f\u4e0e\u6837\u672c\u8981\u6c42\u3011<\/strong><\/p>\n

\u2022 \u5ba2\u6237\u63d0\u4f9b\u6837\u672c\u7c7b\u578b\u3001\u7269\u79cd\u3001\u5904\u7406\u5206\u7ec4\u548c\u9884\u671f\u6bd4\u8f83\u65b9\u5f0f\u3002\u82e5\u5df2\u6709\u76ee\u6807\u9776\u70b9\u6216\u5185\u53c2\u57fa\u56e0\u8981\u6c42\uff0c\u53ef\u4e00\u5e76\u63d0\u4f9b\u3002<\/p>\n

\u2022 \u7ec6\u80de\u6837\u672c\u5efa\u8bae\u22651\u00d710^5\u4e2a\u7ec6\u80de\uff1b\u7ec4\u7ec7\u6837\u672c\u5efa\u8bae\u226510 mg\uff1b\u63d0\u53d6\u597d\u7684DNA\u5efa\u8bae\u603b\u91cf\u226550 ng\uff0c\u6d53\u5ea6\u548c\u7eaf\u5ea6\u6ee1\u8db3\u5e38\u89c4PCR\u8981\u6c42\u3002<\/p>\n

\u2022 \u540c\u4e00\u9879\u76ee\u4e2d\u5e94\u5c3d\u91cf\u7edf\u4e00\u91c7\u6837\u3001\u88c2\u89e3\u3001DNA\u63d0\u53d6\u548c\u5b9a\u91cf\u6d41\u7a0b\uff0c\u907f\u514d\u56e0\u7ebf\u7c92\u4f53DNA\u4e0e\u6838DNA\u56de\u6536\u6548\u7387\u4e0d\u540c\u9020\u6210\u5047\u6027\u5dee\u5f02\u3002<\/p>\n

\u2022 DNA\u6837\u672c\u5efa\u8bae4 \u00b0C\u77ed\u9014\u8fd0\u8f93\u6216\u221220 \u00b0C\u4fdd\u5b58\u8fd0\u8f93\uff1b\u7ec6\u80de\u6216\u7ec4\u7ec7\u6837\u672c\u53ef\u91c7\u7528\u5e72\u51b0\u8fd0\u8f93\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002<\/p>\n

\u3010\u7ed3\u679c\u8f93\u51fa\u3011<\/strong><\/p>\n

\u62a5\u544a\u53ef\u8f93\u51famtDNA\u9776\u70b9\u62f7\u8d1d\u6570\u3001\u6838\u57fa\u56e0\u5185\u53c2\u62f7\u8d1d\u6570\u3001mtDNA\/nDNA\u6bd4\u503c\u3001mtDNA\u62f7\u8d1d\u6570\/\u7ec6\u80de\u3001\u91cd\u590d\u5b54CV\u3001\u7f6e\u4fe1\u533a\u95f4\u3001\u7ec4\u95f4\u76f8\u5bf9\u53d8\u5316\u500d\u6570\u53ca\u8d28\u63a7\u8bf4\u660e\u3002\u82e5\u7528\u4e8e\u65b9\u6cd5\u5b66\u9a8c\u8bc1\uff0c\u53ef\u8fdb\u4e00\u6b65\u63d0\u4f9b\u9776\u70b9\u7279\u5f02\u6027\u3001\u6269\u589e\u6548\u7387\u4e00\u81f4\u6027\u3001\u91cd\u590d\u6027\u3001\u7ebf\u6027\u8303\u56f4\u548c\u4e0d\u540c\u8f93\u5165\u91cf\u4e0b\u7684\u7a33\u5b9a\u6027\u8bc4\u4f30\u3002<\/p>\n

\u3010\u7ed3\u679c\u5c55\u793a\u5f62\u5f0f\u793a\u4f8b\u3011<\/strong><\/p>\n\n\n\n\n\n\n
\u6837\u672c\u7c7b\u578b<\/strong><\/td>\n\u8f93\u51fa\u6307\u6807<\/strong><\/td>\n\u7ed3\u679c\u89e3\u91ca<\/strong><\/td>\n<\/tr>\n
\u6b63\u5e38\u5bf9\u7167\u7ec4<\/td>\nmtDNA\/nDNA\u6bd4\u503c\u3001mtDNA copies\/cell<\/td>\n\u5efa\u7acb\u672c\u6279\u6b21\u6837\u672c\u7684\u53c2\u8003\u8303\u56f4\u3002<\/td>\n<\/tr>\n
\u5904\u7406\u7ec4\u6216\u75be\u75c5\u7ec4<\/td>\n\u76f8\u5bf9\u5bf9\u7167\u7ec4\u7684\u5347\u9ad8\/\u964d\u4f4e\u500d\u6570<\/td>\n\u5224\u65ad\u7ebf\u7c92\u4f53\u62f7\u8d1d\u6570\u662f\u5426\u53d1\u751f\u53d8\u5316\u3002<\/td>\n<\/tr>\n
\u91cd\u590d\u6837\u672c\/\u590d\u5b54<\/td>\nCV\u3001\u7f6e\u4fe1\u533a\u95f4\u3001\u5206\u7fa4\u56fe<\/td>\n\u8bc4\u4ef7\u6570\u636e\u7a33\u5b9a\u6027\u548c\u5206\u533a\u5224\u8bfb\u8d28\u91cf\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

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